Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species

El-Bondkly, Ahmed Mohamed Ahmed; El-Gendy, Mervat Morsy Abbas Ahmed; Bassyouni, Rasha H.

doi:10.1007/s10482-012-9772-5

Cited by 42 publications

(28 citation statements)

References 38 publications

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“…Besides antibacterial activity, antifungal activity has also been described for prodigiosin against dermatophytic fungi that are difficult to treat. According to El-Bondkly et al (2012), prodigiosin inhibits the spores of dermatophytic fungi, such as Microsporum cookie (MIC = 2.3 lg/ mL), M. ajelloi (MIC = 2.3 lg/mL) and Trichophyton longfeuseus (MIC = 8.1 lg/mL).…”

Section: Discussionmentioning

confidence: 99%

“…A growing number of studies have investigated prodigiosin in recent years and have demonstrated antimicrobial, antimalarial and antitumor properties as well as the induction of apoptosis in T and B lymphocytes. These properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries (El-Bondkly et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

Lapenda

Silva

Vicalvi

et al. 2014

World J Microbiol Biotechnol

133

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Prodigiosin is an alkaloid and natural red pigment produced by Serratia marcescens. Prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in T and B lymphocytes. These properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. The aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. The red pigments produced by S. marcescens exhibited absorption at 534 nm, Rf of 0.59 and molecular weight of 323 m/z. Antimicrobial activity was tested against oxacillin-resistant Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Acinetobacter sp. and oxacillin-resistant S. aureus. The standard antibiotics employed were ampicillin, chloramphenicol, gentamicin and oxacillin. The disc-diffusion tests demonstrated significant inhibition zones for S. aureus (35 ± 0.6), E. faecalis (22 ± 1.0) and S. pyogenes (14 ± 0.6). However, prodigiosin showed resistance to E. coli, P. aeruginosa and acinetobacter, where no significant formation of inhibitory halos were observed. We determined the inhibitory minimum concentrations and bactericidal for 20 strains of oxacillin-resistant S. aureus (ORSA). The pattern was the antibiotic oxacillin. The minimum inhibitory concentrations observed ranged from 1, 2 and 4.0 μg/mL, respectively, while the minimum bactericidal concentrations ranged from 2, 4, 8 and 16 μg/mL. The S. marcescens prodigiosin produced by showed bactericidal and bacteriostatic effect showing promising antimicrobial activity and suggesting future studies regarding its applicability in antibiotics therapies directed ORSA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

Lapenda

Silva

Vicalvi

et al. 2014

World J Microbiol Biotechnol

133

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“…For amplification of the nearly complete 16S rRNA gene the eubacterial primers 27f and 1492r were used [23]. The conditions for this PCR were applied according to El-Bondkly et al [24]. PCR products were checked for correct length on a 1% Tris-borate-EDTA (TBE) agarose gel (1% agarose, 8.9 mM Tris, 8.9 mM borate, 0.2 mM EDTA), stained with ethidium bromide and visualized under UV illumination.…”

Section: Genomic Dna Preparation and 16s Rdna Sequencingmentioning

confidence: 99%

“…ESRAA-301097. Manfio et al [34] and El-Bondkly et al [24] reported that the description of Streptomyces species must be based on a combination of genotypic and phenotypic data and if sufficient evidence is provided that an unknown is clearly different in both genotypic and phenotypic features, novel species can be described.…”

Section: Molecular Identification Of Hyper-xylanase Producing Strain mentioning

confidence: 99%

Optimization of Solid State Fermentation and Leaching Process Parameters for Improvement Xylanase Production by Endophytic Streptomyces sp. ESRAA-301097

El-Gendy¹

2014

J Microb Biochem Technol

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In the course of our searching program on the microbial endophytes of medical plants (Cympobogon proximus, Anethum graveolens, Artemisia judaica and Corchorus olitorius), the endophytic strain Streptomyces sp. ESRAA-301097 derived from Cympobogon proximus proved to be the hyper xylanase producer. Screening of various locally available agro-industrial residues as substrate support for xylanase production under SSF exhibited a mixture of wheat bran (WB); sugarcane bagasse (SCB) with corncob (CC) at a ratio of 0.5:1:1 as the efficient inducer for the induction of ESRAA-301097 xylanase production as it gave the highest enzyme productivity (2364 Ugds -1 ) at the 4th day of fermentation when compared to individual WB, SCB or CC (1167, 1241 or 1404 Ugds -1 ) after 3, 4 and 4 days of incubation. Xylanase production was enhanced to 3819 Ugds -1 after optimizing the physical process parameters including temperature 30-40°C, pH 7.0, an inoculum level of 10 7 spore gds -1 , 80-85 % initial moisture content and substrate particle size of 800 µm. An overall 23.96 % increase in enzyme production was attained with a mixture of soybean and corn steep solid as a nitrogen source but no enhancement was obtained with any of carbon or metal supplementation. Whereas xylanase yield was elucidated to 5709.2 Ugds -1 by adding Tween 20, SDS repressed its production to 750.29 Ugds -1 . The optimized leaching parameters for effective extraction of xylanase (6312.45 Ugds -1 ) from the fermented solid mixture were found to be citrate buffer (0.1 M, pH 4.0) containing 0.2% Tween 80 as leaching agent, extractant volume 1:8 -1:10 (w/v), soaking time 120 min, leaching pH 4 and leaching temperature 50°C under agitation at 150 rpm. The overall level of 44.61-fold purification of Streptomyces sp. ESRAA-301097 and xylanase recovery 32.52% were achieved with specific activity of 493.48 Umg -1 . The purified enzyme showed a single protein band on SDS-PAGE indicating the monomeric nature of the enzyme with molecular weight ~31.5 kDa. Furthermore, whereas the inhibitors of cysteine protease (1, 10-phenanthroline and Dithiothreitol), metaloprotease (EDTA and EGTA) and thioprotease (iodoacetamide and p-chloromercuribenzoate) had no to minor effects on xylanase activity, the serine protease inhibitor (PMSF) markedly decreased it.

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“…Prodigiosin-like pigments (PLPs) compounds isolated from endophytic marine Actinomycetes recovered from the Egyptian marine sponge Latrunculia corticata exhibited significant cytotoxic activities against three human cancer cell lines: colon cancer cell line (HCT-116), liver cancer cell line (HEPG-2) and breast cancer cell line (MCF-7) 25 .…”

Section: Fatty Acidsmentioning

confidence: 99%

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Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species

Cited by 42 publications

References 38 publications

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

Optimization of Solid State Fermentation and Leaching Process Parameters for Improvement Xylanase Production by Endophytic Streptomyces sp. ESRAA-301097

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