Overproduction of Hydroxymethylpyrimidine by a Thiamine Regulatory Mutant of Escherichia coli

Iwashima, Akio; Takahashi, Kisaburo; Nose, Yoshitsugu

doi:10.5925/jnsv1954.17.43

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…During the course of this work it was observed that strains carrying an insertion in thiM excreted a compound able to satisfy the requirement of HMP-requiring strains. It had been previously observed by others that certain mutants that are impaired for thiamine synthesis accumulate the HMP moiety and excrete it into the growth medium (18,29). To determine if the biologically active compound was HMP, it was purified from 2 liters of DM176 (thiM938::MudJ) as described in Materials and Methods.…”

Section: Resultsmentioning

confidence: 99%

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Identification and characterization of an operon in Salmonella typhimurium involved in thiamine biosynthesis

Petersen

Downs

1997

J Bacteriol

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Thiamine pyrophosphate (TPP) is synthesized de novo in Salmonella typhimurium and is a required cofactor for many enzymes in the cell. Five kinase activities have been implicated in TPP synthesis, which involves joining a 4-methyl-5-(␤-hydroxyethyl)thiazole (THZ) moiety and a 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety. We report here identification of a 2-gene operon involved in thiamine biosynthesis and present evidence that the genes in this operon, thiMD, encode two previously identified kinases, THZ kinase and HMP phosphate (HMP-P) kinase, respectively. We further show that this operon belongs to the growing class of genes involved in TPP synthesis that are transcriptionally regulated by TPP. Our data are consistent with ThiM being a salvage enzyme and ThiD being a biosynthetic enzyme involved in TPP synthesis, as previously suggested.

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Section: Resultsmentioning

confidence: 99%

“…HMP was purified by modifying a previously reported procedure (18). Briefly, 1 ml of an overnight culture of DM176 (thiM938::MudJ) was used to inoculate 2 liters of minimal gluconate medium and left stagnant to grow for 2 days at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of an operon in Salmonella typhimurium involved in thiamine biosynthesis

Petersen

Downs

1997

J Bacteriol

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“…Thiamine is produced by yeasts (3), molds (6), fungi (9), and bacteria (2,10,16). The biosynthetic pathway of the pyrimidine moiety of thiamine (B1 pyrimidine) has not been fully elucidated.…”

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confidence: 99%