Overproduction of Laccase by the White-Rot Fungus Pleurotus ostreatus Using Apple Pomace as Inducer

Abstract

Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among various conditions, the best concentration of apple pomace and cultivation time for the production of laccase by P. ostreatus was 2.5% and 9 days, respectively. Reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1, pox3, pox4, poxc, poxa3, and poxa1b, revealed a clear effect of apple pomace on transcription induction. Our findings reveal that the use of apple pomace can be a model for the valuable addition of similar wastes and for the development of a solid-state fermenter and commercial production of oyster mushroom P. ostreatus.

Fungal Laccase Production from Lignocellulosic Agricultural Wastes by Solid-State Fermentation: A Review

“…It was indicated that food waste was suitable for laccase production, and the maximum laccase activity reached 54,000 U/L in a 15 L bioreactor after 8 days [89]. Laccase produced by Pleurotus ostreatus was significantly increased by the addition of apple pomace, where the maximal laccase production was increased to 114.64 U/mL with 2.5% (w/v) apple pomace compared with 76.81 U/mL of laccase production without apple pomace addition [93]. In fact, the mass proportion of lignocellulosic materials in SF medium was much lower than that in SSF medium, and their dominant role in SF is as an inducer.…”

Characterization of the Solid-State and Liquid Fermentation for the Production of Laccases of Pleurotus ostreatus

“…This activity was approximately 2.8 times (30.24 U/ml) and 0.9 times (60.49 U/ml) higher than that of P. ostreatus with 5% (w/v) and without apple pomace, respectively. These results suggest that P. ostreatus might use the nutrient content of apple pomace (rich in carbohydrates, dietary fiber, and minerals) without laccase activity in the initial stages of cultivation (approximately 4 days) [93].…”

Evaluation of nutraceuticals including vitamin B12and xenobiotic degradation capacity ofPleurotusspecies

“…Surprisingly, we also assayed laccase concentration in liquid mycelia growth medium and it was in accordance of many previous studies (Xie et al, 2016; Inacio et al, 2015). The laccase production could be improved by supplementing in liquid growth medium (Park et al, 2014).…”