Overproduction of single cell oil from xylose rich sugarcane bagasse hydrolysate by an engineered oleaginous yeast Rhodotorula mucilaginosa IIPL32

Bandhu, Sheetal; Bansal, Neha; Dasgupta, Diptarka; Junghare, Vivek; Sidana, Arushdeep; Kalyan, Gazal; Hazra, Saugata; Ghosh, Debashish

doi:10.1016/j.fuel.2019.115653

Cited by 18 publications

(6 citation statements)

References 57 publications

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“…Furthermore, oleaginous yeasts exhibit different mechanisms for the conversion of glucose and acetate during lipogenesis. In the case of glucose as a substrate, depleted elements from the medium such as nitrogen (Bandhu et al 2019), phosphate (Wang et al 2018a), or sulfur (Wu et al 2011) usually trigger lipogenesis. Silverman et al (2016) summarized the biosynthesis of fatty acids in oleaginous yeasts.…”

Section: Lipid Biogenesis In Oleaginous Yeastsmentioning

confidence: 99%

Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production

Sanya

Onésime

Passoth

et al. 2021

Appl Microbiol Biotechnol

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Strains of the yeast genus Blastobotrys (subphylum Saccharomycotina) represent a valuable biotechnological resource for basic biochemistry research, single-cell protein, and heterologous protein production processes. Species of this genus are dimorphic, non-pathogenic, thermotolerant, and can assimilate a variety of hydrophilic and hydrophobic substrates. These can constitute a single-cell oil platform in an emerging bio-based economy as oleaginous traits have been discovered recently. However, the regulatory network of lipogenesis in these yeasts is poorly understood. To keep pace with the growing market demands for lipidderived products, it is critical to understand the lipid biosynthesis in these unconventional yeasts to pinpoint what governs the preferential channelling of carbon flux into lipids instead of the competing pathways. This review summarizes information relevant to the regulation of lipid metabolic pathways and prospects of metabolic engineering in Blastobotrys yeasts for their application in food, feed, and beyond, particularly for fatty acid-based fuels and oleochemicals. Key points• The production of biolipids by heterotrophic yeasts is reviewed. • Summary of information concerning lipid metabolism regulation is highlighted.• Special focus on the importance of diacylglycerol acyltransferases encoding genes in improving lipid production is made.

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Section: Lipid Biogenesis In Oleaginous Yeastsmentioning

confidence: 99%

Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production

Sanya

Onésime

Passoth

et al. 2021

Appl Microbiol Biotechnol

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“…It has wide biotechnological applications. Depending on the strain, R. mucilaginosa exhibits the ability to biosynthesize exopolysaccharides [ 20 ], patulin-degrading aldolases [ 24 ], carotenoids [ 25 ], proteases [ 26 ], and Single Cell Oil (SCO) [ 27 ], and can be used as a biological agent in biocontrol [ 28 ]. The yeast R. mucilaginosa is also characterized by detoxification mechanisms that can neutralize the toxic effects of various elements (including selenium) present in the culture medium by forming selenium nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Diversity of Red Yeasts in Various Regions and Environments of Poland and Biotechnological Potential of the Isolated Strains

Kot,

Sęk,

Kieliszek

et al. 2023

Appl Biochem Biotechnol

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Due to the growing demand for natural carotenoids, researchers have been searching for strains that are capable of efficient synthesis of these compounds. This study tested 114 red yeast strains collected from various natural environments and food specimens in Poland. The strains were isolated by their ability to produce red or yellow pigments in rich nutrient media. According to potential industrial significance of the carotenoids, both their total production and share of individual carotenoids (β-carotene, γ-carotene, torulene, and torularhodin) were analyzed. The total content of carotenoid pigments in the yeast dry matter ranged from 13.88 to 406.50 µg/g, and the percentages of individual carotenoids highly varied among the strains. Most of the yeast isolates synthesized torulene at the highest amount. Among the studied strains, isolates with a total carotenoid content in biomass greater than 200 µg/g and those containing more than 60% torularhodin were selected for identification (48 strains). The identified strains belonged to six genera: Rhodotorula, Sporidiobolus, Sporobolomyces, Buckleyzyma, Cystofilobasidium, and Erythrobasidium. The largest number of isolates belonged to Rhodotorula babjevae (18), Rhodotorula mucilaginosa (7), Sporidiobolus pararoseus (4), and Rhodotorula glutinis (4).

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“…Other physical parameters also affect the accumulation of lipids, such as light intensity, temperature, pH, dissolved oxygen and culture agitation [113][114][115]. Metabolic regulation in oleaginous microorganisms is used as a biological tool to develop metabolic engineering strains to increase lipid production and develop robust strains that have other desired characteristics, such as survival under adverse conditions, extension of the substrate spectrum, and favoring downstream processing to lipid recovery [77,110,[116][117][118].…”

Section: Strain Developmentmentioning

confidence: 99%

Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects

Silva

Martins

Moreira

et al. 2023

Foods

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The use of lignocellulosic biomass as a raw material for the production of lipids has gained increasing attention, especially in recent years when the use of food in the production of biofuels has become a current technology. Thus, the competition for raw materials for both uses has brought the need to create technological alternatives to reduce this competition that could generate a reduction in the volume of food offered and a consequent commercial increase in the value of food. Furthermore, the use of microbial oils has been studied in many industrial branches, from the generation of renewable energy to the obtainment of several value-added products in the pharmaceutical and food industries. Thus, this review provides an overview of the feasibility and challenges observed in the production of microbial lipids through the use of lignocellulosic biomass in a biorefinery. Topics covered include biorefining technology, the microbial oil market, oily microorganisms, mechanisms involved in lipid-producing microbial metabolism, strain development, processes, lignocellulosic lipids, technical drawbacks, and lipid recovery.

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Overproduction of single cell oil from xylose rich sugarcane bagasse hydrolysate by an engineered oleaginous yeast Rhodotorula mucilaginosa IIPL32

Cited by 18 publications

References 57 publications

Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production

Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production

Diversity of Red Yeasts in Various Regions and Environments of Poland and Biotechnological Potential of the Isolated Strains

Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects

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