Overripening of ovulated eggs in goldfish

Formacion, M. J.; Hori, Reiji; Lam, T.J.

doi:10.1016/0044-8486(93)90258-z

Cited by 31 publications

(31 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Progesterone Microscopic examination of ovulated eggs from fish immersed for 12h postovulation in different doses of P revealed that the eggs were mainly in an early stage of overripening similar to those observed at around 6h postovulation by Formacion et al (1993), while the control eggs did not look different from those reported for 12h postovulation. The effect of the P treatment on fertilization and hatching rates of eggs is presented in Fig.…”

Section: In Vivo Experimentsmentioning

confidence: 87%

“…The selection and care of gravid goldfish, as well as the induction of ovulation, have been described previously (Formacion et al 1993). Gravid female goldfish, Carassius auratus, of the fringetail variety were induced to ovulate by an injection of human chorionic gonadotropin (HCG) IU g bwt-1).…”

Section: Experimental Animals and Induction Of Ovulationmentioning

confidence: 99%

“…Percentage fertilization and percentage of fertilized eggs that hatched were determined. A small sample of the eggs was also observed under a binocular microscope to determine the stage of overripening (as described in Formacion et al 1993).…”

Section: In Vivo Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Formacion

Venkatesh

Tan

et al. 1995

Fish Physiol Biochem

View full text Add to dashboard Cite

Changes in steroid hormone levels in the serum and ovarian fluid during overripening were studied in goldfish. Ovulated eggs retained in the ovarian cavity became overripe at around 12h after ovulation based on loss of fertilizability, with advanced degeneration by 24h. Blood and ovarian fluid were taken at 0, 3, 6, 12, 18 and 24h after ovulation. Both serum and ovarian fluid progesterone (P) showed a highly significant decline by 18h with a further decline by 24h; P levels were higher in the ovarian fluid. Serum 17α,20β-dihydroxy-4-pregnen-3-one(17,20β-P) levels showed a progressive and more rapid decline, decreasing significantly by 12h with further decreases by 18h and 24h. Serum testosterone (T) levels increased significantly at 3h and remained high till 18h, thereafter they declined to the 0h level. No significant changes in estradiol-17β (E2) levels were observed in the serum, except for a significant difference between 6 and 24h. There were no significant changes in ovarian fluid E2, T or 17,20β-P levels.The postovulatory follicles (POFs) showed degenerative changes which corresponded to the decline in P and 17,20β-P. The hypothesis that overripening may be associated with declining levels of P or 17,20β-P was tested in vivo by immersing just ovulated females (0h) in P or 17,20β-P solutions for 12h, and in vitro by immersing just-ovulated eggs (0h) in ovarian fluid with an anti-serum against P (Anti-P). In the former, P at 0.05 ppm significantly enhanced the fertilization rate of ovulated eggs stripped from the females at 12h while 17,20β-P did not produce a significant effect at the tested concentrations (0.025 and 0.05 ppm) on the fertilization rate. In the latter, anti-P significantly lowered the fertilization rate of 0h ovulated eggs after 6h incubation. The evidence suggests a role of P in the maintenance of viability of ovulated eggs.

show abstract

Section: In Vivo Experimentsmentioning

confidence: 87%

Section: Experimental Animals and Induction Of Ovulationmentioning

confidence: 99%

See 1 more Smart Citation

Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Formacion

Venkatesh

Tan

et al. 1995

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…For each measured parameter, means sharing a common alphabetical symbol do not significantly differ. Rizzo et al, 2003Rasines et al, 2012Espinach et al, 1984Formacion et al, 1993Samarin et al, 2011aSakai et al 1975Bry, 1981;Springate et al, 1984;Lahnsteiner, 2000;Azuma et al 2003;Bonnet et al, 2003;Aegerter and Jalabert, 2004;Samarin et al, 2011 Caspian brown trout (Salmo trutta caspius) 30-40 days Bahrekazemi et al, 2010 different fish species (Table 3). Increasing eyed-egg mortality and malformation rates can be interpreted as post-ovulatory oocyte ageing is accompanied by biochemical changes inside the eggs (Craik and Harvey, 1984;Lahnsteiner, 2000) as well as by the leakage of a number of oocyte components, such as protein fragments, into the ovarian fluid (Rime et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Through over-ripening, major morphological, physiological, biochemical, histological, cellular and molecular changes occur inside the eggs and ovarian fluid that negatively affect the egg fertilizing ability and larval developmental stages (e.g., Nomura et al, 1974;Craik and Harvey, 1984;Formacion et al, 1993;Lahnsteiner, 2000;Aegerter et al, 2005). The time period during which the eggs remain viable after ovulation differs from species to species and is largely dependent on temperature (e.g., Piper et al, 1982;Espinach et al, 1984;Harvey and Kelly, 1984;Formacion et al, 1993;Legendre et al, 2000;Rizzo et al, 2003;Samarin et al, 2008;Bahrekazemi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Samarin¹,

Blecha²,

Bytyutskyy³

et al. 2015

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The effect of post-ovulatory oocyte ageing on egg quality was assessed in pikeperch Sander lucioperca to identify the reliable and the best post-ovulatory stripping time. Ovulated eggs were retained in the ovarian cavity for 0-3, 3-9, 6-12 and 12-18 hours post-ovulation (HPO) prior to fertilization. Fertilization, hatching and embryo mortality rates as well as the occurrence of larval malformations and ploidy anomalies were considered as indices for egg quality. The results indicated that the fertilization and hatching rates remained nearly constant, at approximately 80%, for the eggs retained in the ovarian cavity between 0-18 hours after ovulation. Post-ovulatory oocyte ageing did not affect the embryo mortality or the occurrence of larval malformations at least during the 12-hour experimental period. However, the incidence of triploidization in the larvae increased over time from 1.6% at 0-3 HPO to 5% in more aged oocytes at 6-18 HPO. Egg quality retention for at least 12 hours after ovulation appears to be helpful for pikeperch breeding programs by providing synchronous artificial egg insemination and thereby easing hatchery management.

show abstract