Leaching of dissolved organic matter (DOM) from pastoral soils is increasingly seen as an important but poorly understood process. This paper examined the relationship between soil chemical properties, microbial activity and the losses of dissolved organic carbon (DOC) and nitrogen (DON) through leaching from six pasture soils. These soils differed in carbon (C) (4.6-14.9%) and nitrogen (N) (0.4-1.4%) contents and in the amount of organic C and N that had accumulated or been lost in the preceding 20+ years (i.e. −5131 to +1624 kg C ha −1 year −1 and −263 to +220 kg N ha −1 year −1 , respectively). The paper also examined whether between-soil-type differences in DOC and DON leaching was a major explanatory factor in the observed range of soil organic matter (SOM) changes in these soils. Between 280 and 1690 kg C ha −1 year −1 and 28-117 kg N ha −1 year −1 leached as DOC and DON, respectively, from the six soils in a lysimeter study, with losses being greater from two poorly drained gley soils. Losses of C and N of this magnitude, while at the upper end relative to published data, could not fully explain the losses at Rawerawe, Bruntwood and Lepperton sites reported by Schipper et al. (2007). The study highlights the leaching of DOM as a significant pathway of loss of C and N in pasture soils that is often ignored or given little attention in predictive models and nutrient budgeting. Leaching losses of DOC and DON alone, or in combination with slightly increased respiration losses of SOM given a 0.2 • C increase in the mean annual soil temperature, do not fully explain long-term changes in the SOM observed at these sites. When soils examined in the present study were separated on the basis of drainage class, the losses of DOC by leaching were correlated with both total and hot-water extractable C (HWC), the latter being a measure of the labile SOM fraction. Basal microbial CO 2 respiration rates, which varied between 1 and 3.5 μg CO 2 -C g −1 soil hour −1 in surface soils (0-75-mm depth), was also linked to HWC and the quantities of C lost as DOC. Adoption of the HWC method as an approach that could be used as a proxy for the direct measurement of the soil organic C lost by leaching as DOC or respired needs to be examined further with a greater number of soils. In comparison, a poor relationship was found between the hot-water extractable N (HWN) and loss of DON by leaching, despite HWN previously being shown to be a measure of the mineralizable pool of N in soils, possibly reflecting the greater competition for N than C in these soils.