Oversulfated chondroitin sulfate interaction with heparin-binding proteins: New insights into adverse reactions from contaminated heparins

Li, Boyangzi; Suwan, Jiraporn; Martin, Jeffrey G.; Zhang, Fuming; Zhang, Zhenqing; Hoppensteadt, Debra; Clark, Marin K.; Fareed, Jawed; Linhardt, Robert J.

doi:10.1016/j.bcp.2009.04.012

Cited by 70 publications

(69 citation statements)

References 21 publications

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“…The interactions of complement proteins with OSCS and CS-A were compared by SPR (Li et al (2009)). The OSCS and CS-A were immobilized on a SPR chip.…”

Section: The Role Of Chondroitin Sulfate and Its Derivatives In Complmentioning

confidence: 99%

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Ijaz

et al. 2015

Carbohydrate Polymers

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“…The interactions of complement proteins with OSCS and CS-A were compared by SPR (Li et al (2009)). The OSCS and CS-A were immobilized on a SPR chip.…”

Section: The Role Of Chondroitin Sulfate and Its Derivatives In Complmentioning

confidence: 99%

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Ijaz

et al. 2015

Carbohydrate Polymers

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“…Until then, contaminants like OSCS could not be identified using conventional screening tests described in various pharmacopoeias. Therefore, new methods such as NMR spectroscopy [28,[51][52][53][54], capillary electrophoresis [55][56][57], strong-anion-exchange high-performance liquid chromatography [58], infrared (IR), near-infrared reflectance (NIR), and Raman spectroscopy [59][60][61], one-dimensional cellulose acetate plate electrophoresis and polyacrylamide gel electrophoresis [62,63], potentiometric polyanion sensors [64], and anticoagulant time assays [65][66][67] were developed and partly included in the international pharmacopoeias for identification and quantification purposes of potential contaminants in heparin.…”

Section: Application To Unfractionated Heparinmentioning

confidence: 99%

Quantitative NMR spectroscopy of biologically active substances and excipients

Beyer

Diehl²,

Holzgrabe

2010

Bioanal Rev

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Biologically active ingredients and excipients are the essentials of a drug formulation, such as a tablet, dragee, solution, etc. Quality control of such substances thus plays a pivotal role in the production process of pharmaceutical drugs. Since these agents often exhibit complex structures, consist of multiple components, or lack of a chromophore, traditional means of characterization are often not feasible. Furthermore, substances of small molecular weight or strong polar character generally exhibit poor chromatographic properties, thus, conventional procedures such as high-performance liquid chromatography are often not applicable. Instead, quantitative nuclear magnetic resonance (qNMR) spectroscopy has emerged as an alternative or orthogonal method in drug analysis. In this review, we elaborate on the application of qNMR to three important classes of biological substances, namely polysaccharides, amino acids, and lipids, and demonstrate the benefits of this modern tool in contrast to traditional techniques.

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“…Patients treated with OSCS-contaminated Hep experienced angioedema, hypertension, swelling of the larynx, and in some cases, death [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…1). Hep has been used clinically as an anticoagulant and antithrombotic agent for many years [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Novel and highly sensitive mixed‐polymeric electrokinetic chromatography system for determination of contaminants and impurities of heparin samples

et al. 2010

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A mixed-polymeric electrokinetic chromatography system has been developed for the simultaneous determination of a contaminant like oversulfated condroitin sulfate (OSCS) and impurities expressed as dermatan (Der) in heparin (Hep) samples. The EKC system consisted of 0.5% w/v polymeric β-CD, 0.4% w/v tetronic(®) 1107 and 400 mM tris-phosphate buffer at pH 3.5. The optimized electrophoretic conditions included the use of an uncoated-silica capillary of 50 cm of total length and 75 μm id, an applied voltage of -7 kV, a temperature of 30°C and 200 nm UV-detection. The highly sensitive method developed showed low values of LOD, 0.07% w/w (0.07 μg/mL) (OSCS) and 0.1% w/w (0.1 μg/mL) (Der), and values of LOQ 0.2% w/w (0.2 μg/mL) (OSCS) and 0.3% w/w (0.3 μg/mL) (Der) with a concentration level of Hep sample as low as 0.1 mg/mL. Additional parameters of validation such as specificity, linearity, accuracy, and robustness were evaluated according to international guidelines. Owing to its simplicity, high sensitivity, and reliability, the proposed method can be an advantageous alternative to the traditional methodologies for the analysis of Hep in raw material and specially in finished products because of the low amounts of Hep sample required.

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Oversulfated chondroitin sulfate interaction with heparin-binding proteins: New insights into adverse reactions from contaminated heparins

Cited by 70 publications

References 21 publications

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Quantitative NMR spectroscopy of biologically active substances and excipients

Novel and highly sensitive mixed‐polymeric electrokinetic chromatography system for determination of contaminants and impurities of heparin samples

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