Overview and detectability of the genetic modifications in ornamental plants

Boutigny, Anne-Laure; Dohin, Nicolas; Pornin, David; Rolland, Mathieu

doi:10.1038/s41438-019-0232-5

Cited by 58 publications

(41 citation statements)

References 167 publications

(101 reference statements)

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“…As shown by the example of the GM petunia, there is a risk of misuse of genetically modified biological material developed for research purposes. Furthermore, the risk of finding such material on the market is increased when the transgene provides a trait of commercial interest 28 .…”

Section: Resultsmentioning

confidence: 99%

Targeted MinION sequencing of transgenes

Boutigny¹,

Fioriti²,

Rolland³

2020

Sci Rep

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The presence of genetically modified organisms (GMO) is commonly assessed using real-time PCR methods targeting the most common transgenic elements found in GMOs. Once the presence of GM material has been established using these screening methods, GMOs are further identified using a battery of real-time PCR methods, each being specific of one GM event and usually targeting the junction of the plant genome and of the transgenic DNA insert. If, using these specific methods, no GMO could be identified, the presence of an unauthorized GMO is suspected. In this context, the aim of this work was to develop a fast and simple method to obtain the sequence of the transgene and of its junction with plant DNA, with the presence of a screening sequence as only prior knowledge. An unauthorized GM petunia, recently found on the French market, was used as template during the development of this new molecular tool. The innovative proposed protocol is based on the circularization of fragmented DNA followed by the amplification of the transgene and of its flanking regions using long-range inverse PCR. Sequencing was performed using the Oxford Nanopore MinION technology and a bioinformatic pipeline was developed.

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Section: Resultsmentioning

confidence: 99%

Targeted MinION sequencing of transgenes

Boutigny¹,

Fioriti²,

Rolland³

2020

Sci Rep

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“…Delayed petal senescence was also observed in mutant Japanese morning glory (Ipomoea nil "Violet") plants generated by the CRISPR-Cas9 system that targeted the EPHEMERAL1 (EPH1) gene, encoding the master regulator of petal senescence as an NAC transcription [53]. Flower color is one of the most important commercial traits in ornamental plants [21], and it is derived mainly from flavonoids, carotenoids, and betalains [54]. Currently, flower color modification has been widely applied using genome-editing tools in many plant species.…”

Section: Current Status Of Genome Editing In Ornamental Plantsmentioning

confidence: 99%

“…Since the first report of genetic transformation in ornamental plants in 1987 [20], genetic transformation has been recognized as an important technique for generating new cultivars. Despite at least 50 ornamental plants being transformed [21], very few genetically modified (GM) ornamental plants have been approved and commercialized, and only in a few countries. For instance, both a violet carnation, "Moondust™", and a blue rose, "Applause™", which are GM to accumulate delphinidin-based anthocyanins, were generated and commercialized by the Australian biotechnology company Florigene and the Japanese company SUNTORY, respectively, in the global flower market.…”

Section: Introduction Of Genome-editing Technologiesmentioning

confidence: 99%

Progress and Challenges in the Improvement of Ornamental Plants by Genome Editing

Ahn

Ramya

et al. 2020

Plants

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Biotechnological approaches have been used to modify the floral color, size, and fragrance of ornamental plants, as well as to increase disease resistance and vase life. Together with the advancement of whole genome sequencing technologies, new plant breeding techniques have rapidly emerged in recent years. Compared to the early versions of gene editing tools, such as meganucleases (MNs), zinc fingers (ZFNs), and transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeat (CRISPR) is capable of altering a genome more efficiently and with higher accuracy. Most recently, new CRISPR systems, including base editors and prime editors, confer reduced off-target activity with improved DNA specificity and an expanded targeting scope. However, there are still controversial issues worldwide for the recognition of genome-edited plants, including whether genome-edited plants are genetically modified organisms and require a safety evaluation process. In the current review, we briefly summarize the current progress in gene editing systems and also introduce successful/representative cases of the CRISPR system application for the improvement of ornamental plants with desirable traits. Furthermore, potential challenges and future prospects in the use of genome-editing tools for ornamental plants are also discussed.

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“…Поэтому в последнее время активно развиваются методы генной инженерии, которые позволяют в относительно короткие сроки придать растению новые признаки, которые не могут быть получены при помощи селекции. В настоящее время трансформировано уже более 50 родов декоративных растений (Shibata, 2008;Boutigny et al, 2020). Чаще всего культурные растения трансформируют путём прямой регенерации побегов или соматического эмбриогенеза с использованием агробактериальной трансформации или биобаллистики (Brand, 2006).…”

Section: Introductionunclassified

Genetic engineering as a way to obtain ornamental plants with a changed flower color

Санникова¹

2020

Biotehnologiâ i selekciâ rastenij

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An important trend in the field of floriculture is the creation of new varieties of ornamental plants, among which varieties with unusual color are most in demand. To this end, traditional breeding and selection programs have been successfully applied for many years. However, currently genetic engineering is able to offer an alternative way to obtain new forms and varieties. Anthocyanins belonging to flavonoids, betalains and carotenoids are the main types of pigments that are synthesized in the plant and are responsible for the color of flower petals. The modification of pigment biosynthesis pathways using genetic engineering techniques can produce results that cannot be obtained by traditional breeding. This review presents the main advances in the application of genetic engineering techniques in floriculture using the example of flower color modification. There are several main areas of work with the genes of pigment biosynthesis. Among them, the strategy of suppressing gene expression is used most often. Expression of certain genes is suppressed to prevent pigment synthesis, or vice versa, to eliminate factors that hinder color development. The method of additional heterologous genes insertion to plants lacking them in the pathway of pigment biosynthesis is often used. Genomic editing, in particular by using the CRISPR/Cas system, is also used for color modification, but the application of this method to ornamental plants is a relatively recent innovation. Despite the rapid development of biotechnology, there are obstacles to the distribution of genetically modified plants on the world market. By addressing a number of problems, the production of transgenic ornamental plants may become economically more cost-effective and attractive than the development of new varieties exclusively through traditional breeding methods.

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Overview and detectability of the genetic modifications in ornamental plants

Cited by 58 publications

References 167 publications

Targeted MinION sequencing of transgenes

Targeted MinION sequencing of transgenes

Progress and Challenges in the Improvement of Ornamental Plants by Genome Editing

Genetic engineering as a way to obtain ornamental plants with a changed flower color

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