Overview of cicatricial modulators in glaucoma fistulizing surgery

Oliveira, Camille Moura de; Ferreira, Juliana de Lucena Martins

doi:10.1007/s10792-020-01454-w

Cited by 13 publications

(14 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lack of patient adherence and insufficient success with drop therapy necessitate sustained alternative forms of therapy [ 8 , 9 ]. Surgical interventions such as trabeculectomy are used as invasive forms of therapy [ 10 , 11 ]. In 1969, Molteno et al [ 12 ] introduced glaucoma drainage devices which have been increasingly used in glaucoma surgery ever since.…”

Section: Introductionmentioning

confidence: 99%

“…A complication of draining surgical techniques is a scarring reaction in Tenon’s space, which is characterized by the transformation of Tenon’s fibroblasts into myofibroblasts. Long-term results of these invasive therapies show that fibrotic processes and uncontrolled, excessive and persistent scarring lead to a decrease in aqueous humor outflow, which in turn results in an increase in IOP [ 10 , 11 , 15 , 16 ]. To prevent fibroblast-mediated fibrosis, cytostatic agents such as mitomycin C (MMC) can be used, but they are associated with side effects such as an avascular, infected or rupturing bleb [ 15 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Antibiotic Kitasamycin—A Potential Agent for Specific Fibrosis Preventing Therapy after Fistulating Glaucoma Surgery?

et al. 2023

View full text Add to dashboard Cite

One major complication after fistulating glaucoma surgeries are fibroblast-mediated scarring processes and their specific prevention is key in the development of novel pharmaceutical concepts. Within this study a possible antifibrotic potential of kitasamycin (KM) in a transforming growth factor (TGF)-β1-mediated fibroblast model was evaluated in vitro. Primary ocular fibroblasts were isolated, cultivated and a dose–response test including determination of the half maximal effective concentration (EC50) for KM was conducted. Transformation of fibroblasts into myofibroblasts was induced by TGF-β1and immunofluorescence (IF), and Western blot (WB) analyses were performed with fibroblasts and myofibroblasts. IF analyses were carried out using antibodies against α-smooth muscle actin (α-SMA) and fibronectin, and protein detection of intracellular and extracellular proteins was performed by WB. Using the dose–response test, the viability, cytotoxicity and EC50 of KM after 24 and 48 h were determined. Fibroblasts exposed to various KM concentrations showed no increase in α-SMA and extracellular matrix expression. In TGF-ß1-stimulated myofibroblasts, KM inhibited the expression of α-SMA and fibronectin in a concentration-dependent manner. These findings demonstrate that KM could impair the transformation of fibroblasts into myofibroblasts and the expression of proteins involved in fibrotic processes, representing a potential agent for specific fibrosis prevention in future therapeutic concepts.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Antibiotic Kitasamycin—A Potential Agent for Specific Fibrosis Preventing Therapy after Fistulating Glaucoma Surgery?

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Until now, mitomycin C (MMC) and 5‐fluorouracil (5‐FU) were worldwide anti‐fibrotic therapies to inhibit the postoperative scarring 3 . Nevertheless, given the obnoxious side effects including sclera ischemia, corneal epithelial defects, over‐filtration, and thinning of the overlying conjunctiva, 4 we need a safer and more effective therapeutic agent to inhibit scar formation after GFS.…”

Section: Introductionmentioning

confidence: 99%

Inhibited effect of an RGD peptide hydrogel on the expression of β1‐integrin, FAK, and Akt in Tenon's capsule fibroblasts

Chen

Liang

et al. 2021

J Biomed Mater Res

View full text Add to dashboard Cite

Tenon's capsule fibroblasts are the main cellular components of filtration tract scar that limit the success rate of glaucoma filtration surgery. Scar formation results from infiltration and proliferation of fibroblasts into damaged areas, meanwhile synthesis of extracellular matrix glycoproteins. Integrins are cell surface receptors for extracellular molecules that mediate cell adhesion, spreading, migration, and invasion. They bind their ligands often through recognition of short amino‐acid sequences—arginine‐glycine‐aspartic acid (RGD). Peptides that contain RGD sequence can compete with RGD containing insoluble matrix proteins for binding to the integrin receptor and thus prevent the downstream signaling pathway. Increasing evidence supports that β1‐integrin/focal adhesion kinase (FAK)/Akt signal pathway plays an important role in fibrogenesis and scar formation in different tissues. In consideration of advantages of peptide hydrogel, that is well biocompatibility, gel state, degradability, good drug loading, we designed and fabricated an RGD peptide hydrogel, and hypothesized that it could inhibit the expression of β1‐integrin, FAK, and Akt in Tenon's capsule fibroblasts. Rheology results showed that 1% wt Fmoc‐FFGGRGD peptide solution could self‐assemble into hydrogel. Western blot analysis revealed that there were statistical differences between control group and 1% wt group in β1‐integrin/β‐actin, FAK/β‐actin, Akt/β‐actin respectively (*p < .05). The relative mRNA expression of β1‐integrin, FAK, Akt in control group and 1% wt group were also statistically different respectively (*p < .05). We proved that 1% wt Fmoc‐FFGGRGD self‐assembly peptide hydrogel could inhibit the expression of β1‐integrin, FAK and Akt in Tenon's capsule fibroblasts. It is a promising way to solve scar formation of glaucoma filter channel.

show abstract

“…3,4 In addition, the long term success of surgical incisional procedures in patients with uncontrolled IOP associated with OCP may be compromised, and the risk of complications due to wound healing may be higher. 5,6 Considering that the evidence regarding MP-TLT and OCP is scarce and anecdotal, our purpose is to report first outcomes of MP-TLT in 12 OCP eyes with a minimum follow-up time of 2 years.…”

mentioning

confidence: 99%

“…Glaucoma management in patients with ocular cicatricial pemphigoid (OCP) is challenging, because topical IOP-lowering medications may be ineffective, insufficient, or even deleterious for their damaged ocular surface, specially associated with preserved anti-glaucomatous topical medications 3,4 . In addition, the long term success of surgical incisional procedures in patients with uncontrolled IOP associated with OCP may be compromised, and the risk of complications due to wound healing may be higher 5,6 . Considering that the evidence regarding MP-TLT and OCP is scarce and anecdotal, our purpose is to report first outcomes of MP-TLT in 12 OCP eyes with a minimum follow-up time of 2 years.…”

mentioning

confidence: 99%

MicroPulse Transscleral Laser Therapy in Patients With Glaucoma and Ocular Cicatricial Pemphigoid

Logioco

Delgado

Podesta

et al. 2023

Journal of Glaucoma

View full text Add to dashboard Cite

A non-comparative retrospective case-series study was performed on 6 patients (12 eyes) with a confirmed diagnosis of ocular cicatricial pemphigoid (OCP), treated with MicroPulse Transscleral Laser Therapy (MP-TLT), between January 2018 and 2020, with a minimum follow-up of 2 years, in a private ophthalmology practice with offices in Argentina and Colombia. Mean age was 75±6.3 years (66–83 yrs). The severity of ocular cicatricial pemphigoid remained stable 2 years after MP-TLT. Baseline mean IOP was 19.5±5.9 mmHg (13–30 mmHg) and, 24 months after treatment, IOP decreased to a mean of 13.9±1.9 mmHg (10–18 mmHg). The mean IOP reduction was 25.1% (P: 0.002). The mean number of anti-glaucomatous drugs at baseline was 3.1±0.7, decreasing to 0.6±0.7, 2 years after MP-TLT (P<0.001). MP-TLT may be considered as a non-incisional approach to glaucoma care for patients with ocular surface diseases, prospective long term studies are needed.

show abstract

Overview of cicatricial modulators in glaucoma fistulizing surgery

Cited by 13 publications

References 58 publications

The Antibiotic Kitasamycin—A Potential Agent for Specific Fibrosis Preventing Therapy after Fistulating Glaucoma Surgery?

The Antibiotic Kitasamycin—A Potential Agent for Specific Fibrosis Preventing Therapy after Fistulating Glaucoma Surgery?

Inhibited effect of an RGD peptide hydrogel on the expression of β1‐integrin, FAK, and Akt in Tenon's capsule fibroblasts

MicroPulse Transscleral Laser Therapy in Patients With Glaucoma and Ocular Cicatricial Pemphigoid

Contact Info

Product

Resources

About