Overview of citric acid production from <i>Aspergillus niger</i>

Show, Pau Loke; Oladele, Kehinde Opeyemi; Siew, Qi Yan; Zakry, Fitri Abdul Aziz; Lan, John Chi-Wei; Ling, Tau Chuan

doi:10.1080/21553769.2015.1033653

Cited by 219 publications

(176 citation statements)

References 78 publications

(115 reference statements)

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“…Despite treatment with chelating agents (such as sodium or potassium ferro-cyanide), molasses give low yields of citric acid [1,2]. Therefore, beet molasses requires treatment before use in the production of citric acid.…”

Section: Resultsmentioning

confidence: 99%

“…Citric acid is widely used in the food, beverages, chemical, pharmaceutical, cosmetic and other industries [1]. It is mainly produced from submerged fermentation processes.…”

Section: Introductionmentioning

confidence: 99%

“…The presence of trace metals in toxic concentrations can be a significant problem during the fermentation of substrates into products. Concentrations of trace metals in molasses may be controlled by the treatment with ferrocyanide ion and treated molasses has been extensively used for citric acid production by A. niger [1,6].…”

Section: Introductionmentioning

confidence: 99%

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Citric Acid Production from Nontreated Beet Molasses by a Novel Aspergillus niger Strain: Effects of pH, Sugar and Ingredients

Guc¹,

Erkmen²

2017

J Food Microbiol Saf Hyg

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Effects of factors on the citric acid production from non-treated beet molasses were studied in Aspergillus niger OE55. Maximum amount of citric acid (19.13 and 34.62 g/L) was achieved when the initial pH of fermentation medium was 6.0 from 200 g/L and 150 sugar respectively. Citric acid production and biomass formation continuously increased during fermentation period in the media initially containing 200 g/L sugar. Remaining sugar (from 3.20 to 6.03 g/L) was higher at the end of fermentation in the media initially containing 160 g/L sugar than 200 g/L sugar. Yield of citric acid after 4 days of fermentation were ranged from 0.16 to 0.28 g/g from 160 g/L sugar. The high phosphorus and nitrogen levels stimulated biomass formation and reduced citric acid production. The optimum incubation for maximal citric acid production varies both with the sugar concentration in non-treated molasses and fermentation conditions for the novel A. niger strain. pH of fermentation media cannot have reduced below 4.72 and 3.35 in the media containing 200 and 160 g/L sugar, respectively, during fermentation, therefore citric acid production was not increased over 19.96 and 34.58 g/L respectively. This would be due to the formation of nitrogenous and polysaccharide compounds from molasses. Strain development in the citric acid production from wild strains depends mainly on the fermentation conditions.

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Section: Resultsmentioning

confidence: 99%

“…Citric acid is widely used in the food, beverages, chemical, pharmaceutical, cosmetic and other industries [1]. It is mainly produced from submerged fermentation processes.…”

Section: Introductionmentioning

confidence: 99%

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Citric Acid Production from Nontreated Beet Molasses by a Novel Aspergillus niger Strain: Effects of pH, Sugar and Ingredients

Guc¹,

Erkmen²

2017

J Food Microbiol Saf Hyg

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“…Citric acid can be produced via artificial extraction, chemical synthesis or microbial fermentation [3]. Currently, the development of microbial fermentation for producing citric acid has attracted significant attention due to its high citric acid productivity, and microbial fermentation accounts for approximately 90% of world's citric acid production [4, 5].…”

Section: Introductionmentioning

confidence: 99%

Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger

Chen

et al. 2017

PLoS ONE

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The filamentous ascomycete Aspergillus niger is well known for its ability to accumulate citric acid for the hydrolysis of starchy materials. To improve citric acid productivity, heavy ion beam mutagenesis was utilized to produce mutant A.niger strains with enhanced production of citric acid in this work. It was demonstrated that a mutant HW2 with high concentration of citric acid was isolated after carbon ion irradiation with the energy of 80Mev/μ, which was obvious increase higher than the original strain from liquefied corn starch as a feedstock. More importantly, with the evidence from the expression profiles of key genes and enzyme activity involved in the starch hydrolysis process between original strain and various phenotype mutants, our results confirmed that different transcript levels of key genes involving in starch hydrolysis process between original strain and mutants could be a significant contributor to different citric acid concentration in A.niger, such as, amyR and glaA, which therefore opened a new avenue for constructing genetically engineered A.niger mutants for high-yield citric acid accumulation in the future. As such, this work demonstrated that heavy ion beam mutagenesis presented an efficient alternative strategy to be developed to generate various phenotype microbe species mutants for functional genes research.

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“…(Knuf and Nielsen, 2012;Show et al, 2015;Ward, 2012). (Knuf and Nielsen, 2012;Show et al, 2015;Ward, 2012).…”

mentioning

confidence: 99%

Comprehensive reconstruction and in silico analysis of Aspergillus niger genome‐scale metabolic network model that accounts for 1210 ORFs

Cao

Ouyang

et al. 2016

Biotech & Bioengineering

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Aspergillus niger is one of the most important cell factories for industrial enzymes and organic acids production. A comprehensive genome-scale metabolic network model (GSMM) with high quality is crucial for efficient strain improvement and process optimization. The lack of accurate reaction equations and gene-protein-reaction associations (GPRs) in the current best model of A. niger named GSMM iMA871, however, limits its application scope. To overcome these limitations, we updated the A. niger GSMM by combining the latest genome annotation and literature mining technology. Compared with iMA871, the number of reactions in iHL1210 was increased from 1,380 to 1,764, and the number of unique ORFs from 871 to 1,210. With the aid of our transcriptomics analysis, the existence of 63% ORFs and 68% reactions in iHL1210 can be verified when glucose was used as the only carbon source. Physiological data from chemostat cultivations, C-labeled and molecular experiments from the published literature were further used to check the performance of iHL1210. The average correlation coefficients between the predicted fluxes and estimated fluxes from C-labeling data were sufficiently high (above 0.89) and the prediction of cell growth on most of the reported carbon and nitrogen sources was consistent. Using the updated genome-scale model, we evaluated gene essentiality on synthetic and yeast extract medium, as well as the effects of NADPH supply on glucoamylase production in A. niger. In summary, the new A. niger GSMM iHL1210 contains significant improvements with respect to the metabolic coverage and prediction performance, which paves the way for systematic metabolic engineering of A. niger. Biotechnol. Bioeng. 2017;114: 685-695. © 2016 Wiley Periodicals, Inc.

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Overview of citric acid production from Aspergillus niger

Cited by 219 publications

References 78 publications

Citric Acid Production from Nontreated Beet Molasses by a Novel Aspergillus niger Strain: Effects of pH, Sugar and Ingredients

Citric Acid Production from Nontreated Beet Molasses by a Novel Aspergillus niger Strain: Effects of pH, Sugar and Ingredients

Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger

Comprehensive reconstruction and in silico analysis of Aspergillus niger genome‐scale metabolic network model that accounts for 1210 ORFs

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