Overview of Drug Discovery and Development

Ator, Mark A.; Mallamo, John P.; Williams, Michael

doi:10.1002/0471141755.ph0909s35

“…However, it should be noted that in the ex vivo experiment, chrysin was added to the medium in which lenses were incubated, thus the lenses were exposed directly to this flavonoid action. In the in vivo experiments, when substances are given orally or by intragastric tube, they may be metabolized or bound to plasma proteins and, in consequence, they do not interact directly with the organ [81,82]. Therefore, the discrepancies between our investigation and the ex vivo study are a result of experimental design and the complexity of the living organism.…”

Section: Discussionmentioning

confidence: 93%

Chrysin Reduces Oxidative Stress but Does Not Affect Polyol Pathway in the Lenses of Type 1 Diabetic Rats

Wojnar

¹

,

Zych

²

,

Borymski

³

et al. 2020

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Prolonged hyperglycemia is one of the main causes of reactive oxygen species and free radicals generation in diabetes which may affect various organs, including the eye. Oxidative damage to proteins and lipids in the eye lens could lead to cataract formation. To cope with oxidative stress, the endogenous antioxidative system may be supported by the supplementation of exogenous antioxidants. The aim of this study was to evaluate the effect of chrysin, a natural flavonoid, on oxidative stress and polyol pathway-related markers in the lenses of streptozotocin-induced type 1 male diabetic rats. Chrysin at doses of 50 and 100 mg/kg was administered by gavage for 28 days. This treatment resulted in a decrease in antioxidative enzymes activity and oxidative stress index. Moreover, chrysin administration elevated the reduced glutathione level in the lenses. A decrease in the markers linked to oxidative damage to proteins and lipids in the lenses was noted, especially after treatment with 50 mg/kg of chrysin. Neither of the chrysin doses affected glycemia-related markers in the serum or altered parameters related to the polyol pathway and advanced glycation end-products level in the lenses of diabetic rats. Upon obtaining results, it can be concluded that chrysin reveals antioxidative activity in the lenses but shows no antihyperglycemic or antiglycation properties.

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“…This final output from a laboratory notebook can then be included in a patent application or scientific manuscript. In industry, (e.g., pharmaceutical companies), interim internal reports also exist to support federal regulatory requirements for IND (Investigational New Drug; see Current Protocols article Ator, Mallamo, & Williams, 2006) filing. In performing quality control on such data or researching them for a patent application, it is unfortunately not always obvious from where cited IC 50 values are derived.…”

Section: Hard Copy Paper Notebooksmentioning

confidence: 99%

Laboratory Notebooks and Data Storage

Williams¹,

Bozyczko-Coyne²,

Dorsey³

et al. 2020

CP Essential Lab Tech

Self Cite

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The laboratory notebook, whether in hardcopy or electronic (ELN) format, represents a true, chronological record of a scientist's bench work, incorporating the primary source of all materials and information related to the design, execution, and outcomes (data) for a specific experiment. The notebook content thus represents a precise, legible record of what was done, why, and by whom. It also describes the outcome of the experiment and how this is related to the initial hypothesis on which performing the experiment was based. As an archive, a laboratory notebook allows others previously unassociated with the work to understand what was done and, if necessary, independently recreate the experiments. A laboratory notebook and its associated records (e.g., computer printouts or HPLC traces) also represent the vital record of the conception date of an invention and its reduction to practice, providing a timely and necessary legal record to support and defend patent applications.

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“…This final output from a laboratory notebook can then be included in a patent application or scientific manuscript. In industry, (e.g., pharmaceutical companies) interim internal reports also exist to support federal regulatory requirements for IND (Investigational New Drug; Ator, 2006) filing. In performing quality control on such data or researching them for a patent application, it is unfortunately not always obvious from where cited IC 50 values are derived.…”

Section: Data Replicationmentioning

confidence: 99%

“…Interest in ELNs has increased significantly in the past few years, with many commercial products being available from scientific software, instrumentation, and database-management companies. Major vendors include Waters, CambridgeSoft, Elsevier MDL, and Symyx Technologies (see Ator et al, 2006). Each company approached the ELN from a unique perspective, in the case of Waters, interfacing with their HPLC and Laboratory Informatics Systems; and CambridgeSoft, their ChemDraw software platform.…”

Section: A2mentioning

confidence: 99%

Laboratory Notebooks and Data Storage

Williams¹,

Bozyczko-Coyne²,

Dorsey³

et al. 2008

CP Essential Lab Tech

Self Cite

View full text Add to dashboard Cite

The laboratory notebook, whether in hardcopy or electronic (ELN) format, represents a true, chronological record of a scientist's bench work, incorporating the primary source of all materials and information related to the design, execution, and outcomes (data) for a specific experiment. The notebook content thus represents a precise, legible record of what was done, why, and by whom. It also describes the outcome of the experiment and how this is related to the initial hypothesis on which performing the experiment was based. As an archive, a laboratory notebook allows others previously unassociated with the work to understand what was done and, if necessary, independently recreate the experiments. A laboratory notebook and its associated records (e.g., computer printouts or HPLC traces) also represent the vital record of the conception date of an invention and its reduction to practice, providing a timely and necessary legal record to support and defend patent applications.

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Overview of Drug Discovery and Development

Cited by 11 publications

References 96 publications

Chrysin Reduces Oxidative Stress but Does Not Affect Polyol Pathway in the Lenses of Type 1 Diabetic Rats

Chrysin Reduces Oxidative Stress but Does Not Affect Polyol Pathway in the Lenses of Type 1 Diabetic Rats

Laboratory Notebooks and Data Storage

Laboratory Notebooks and Data Storage

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