Overview of Electrochemical DNA Biosensors: New Approaches to Detect the Expression of Life

Cagnin, Stefano; Caraballo, Marcelo; Guiducci, Carlotta; Martini, Paolo; Ross, Marty; SantaAna, Mark; Danley, David L.; West, Todd H.; Lanfranchi, Gerolamo

doi:10.3390/s90403122

Cited by 125 publications

(76 citation statements)

References 163 publications

(175 reference statements)

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“…Figure reproduced with permission of the publisher from ref. 27 Due to the favourable hybridisation properties of PNAs (higher thermal duplex stability and mismatch discrimination), DNA biosensors with PNA recognition layers have been developed. 28 PNA-derived biosensors are able to distinguish closely related sequences under less restrictive reaction conditions than for DNA.…”

Section: Metal-containing Pnas For Biosensing Purposesmentioning

confidence: 99%

Metal-containing peptide nucleic acid conjugates

2011

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Peptide Nucleic Acids (PNAs) are non-natural DNA/RNA analogues with favourable physicochemical properties and promising applications. Discovered nearly 20 years ago, PNAs have recently re-gained quite a lot of attention. In this Perspective article, we discuss the latest advances on the preparation and utilisation of PNA monomers and oligomers containing metal complexes. These metalconjugates have found applications in various research fields such as in the sequence-specific detection of nucleic acids, in the hydrolysis of nucleic acids and peptides, as radioactive probes or as modulators of PNA˙DNA hybrid stability, and last but not least as probes for molecular and cell biology.

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Section: Metal-containing Pnas For Biosensing Purposesmentioning

confidence: 99%

Metal-containing peptide nucleic acid conjugates

2011

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“…Amplified DNA targets can be analyzed for the presence of SNP by different direct and indirect hybridization strategies including intercalator dyes in allele-specific digital PCR (dPCR) [17], sequence-specific fluorogenic probes [18], electrochemical sensors [19][20][21][22][23][24] and diagnostic nano-materials [25]. Electrochemical sensing is a promising approach for SNP detection which takes advantage of synthetic oligonucleotide probes and the large thermodynamic changes in enthalpy and entropy.…”

Section: Enzyme-based Detection Of Snpmentioning

confidence: 99%

“…nano-particles and fluorescence spectroscopy [17][18][19][20][21][22][23][24][25]. Next several efficient colorimetric assays for SNP sensing in amplified DNA have been developed based on modified gold nano-particles (Au-nps; Figure 2) [26].…”

Section: Enzyme-based Detection Of Snpmentioning

confidence: 99%

Toward Non-Enzymatic Ultrasensitive Identification of Single Nucleotide Polymorphisms by Optical Methods

Astakhova¹

2014

Chemosensors

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Single nucleotide polymorphisms (SNPs) are single nucleotide variations which comprise the most wide spread source of genetic diversity in the genome. Currently, SNPs serve as markers for genetic predispositions, clinically evident disorders and diverse drug responses. Present SNP diagnostics are primarily based on enzymatic reactions in different formats including sequencing, polymerase-chain reaction (PCR) and microarrays. In these assays, the enzymes are applied to address the required sensitivity and specificity when detecting SNP. On the other hand, the development of enzyme-free, simple and robust SNP sensing methods is in a constant focus in research and industry as such assays allow rapid and reproducible SNP diagnostics without the need for expensive equipment and reagents. An ideal method for detection of SNP would entail mixing a DNA or RNA target with a probe to directly obtain a signal. Current assays are still not fulfilling these requirements, although remarkable progress has been achieved in recent years. In this review, current SNP sensing approaches are described with a main focus on recently introduced direct, enzyme-free and ultrasensitive SNP sensing by optical methods.

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“…DNA is one of the most important targets that have been studied during the last decades. Applications range from medical diagnosis and genome sequencing to food, pollution, and environmental analysis [6]. There are several methods to detect DNA: the most widely used is typically based on microarray technique, which consists on nucleic acid hybridization and optical readout [35].…”

Section: Targetsmentioning

confidence: 99%

Integrated biosensors for personalized medicine

Micheli

Boero

Baj-Rossi

et al. 2012

Proceedings of the 49th Annual Design Automation Conference

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Biosensors are heterogenous devices, incorporating biological structures combined with electronics, optical or other readout systems. They have been developed for detecting different biomolecules and/or pathogens and represent a key technology for advanced and pointof-care diagnostics as well as patient monitoring. In this paper we present a systematic classification of biosensors described in literature, particularly focusing on nanotechnology-based sensing. Then, we present our approach to develop electrochemical biosensors for measuring metabolites and anticancer drugs, based on a platform for multiple target detection. This platform is modular and achieves a clear separation between the chemical and the electrical components, thus easing design and manufacturing. It shows superior performance thanks to the excellent properties of electron transfer and selectivity showed by enzymes immobilized on carbon nanotubes.

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Overview of Electrochemical DNA Biosensors: New Approaches to Detect the Expression of Life

Cited by 125 publications

References 163 publications

Metal-containing peptide nucleic acid conjugates

Metal-containing peptide nucleic acid conjugates

Toward Non-Enzymatic Ultrasensitive Identification of Single Nucleotide Polymorphisms by Optical Methods

Integrated biosensors for personalized medicine

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