Overview of Electroporation and Electrofusion

Chang, Donald C.; Saunders, James A.; Chassy, Bruce M.; Sowers, Arthur E.

doi:10.1016/b978-0-08-091727-6.50004-6

Cited by 113 publications

(82 citation statements)

References 17 publications

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“…[1][2][3] Moreover, translocation processes might eventually prove useful in various technological applications, such as rapid DNA sequencing, 4,5 gene therapy, controlled drug delivery, etc. 6 In addition to its biological relevance, the translocation dynamics is also a challenging topic in polymer physics. Accordingly, the polymer translocation has attracted a considerable number of experimental, [7][8][9][10][11][12][13][14] theoretical, [15][16][17][18][19][20][21][22][23][24][25][26][27][28] and numerical studies.…”

Section: Introductionmentioning

confidence: 99%

Polymer translocation through a nanopore: A two-dimensional Monte Carlo study

Luo

Ala-Nissilä

Ying

2006

The Journal of Chemical Physics

119

144

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Polymer translocation through a nanopore induced by adsorption: Monte Carlo simulation of a coarse-grained model J. Chem. Phys. 121, 6042 (2004); 10.1063/1.1785776 This article is copyrighted as indicated in the article. Reuse of AIP content is subject to the terms at: http://scitation. We investigate the problem of polymer translocation through a nanopore in the absence of an external driving force. To this end, we use the two-dimensional fluctuating bond model with single-segment Monte Carlo moves. To overcome the entropic barrier without artificial restrictions, we consider a polymer which is initially placed in the middle of the pore and study the escape time required for the polymer to completely exit the pore on either end. We find numerically that scales with the chain length N as ϳ N 1+2 , where is the Flory exponent. This is the same scaling as predicted for the translocation time of a polymer which passes through the nanopore in one direction only. We examine the interplay between the pore length L and the radius of gyration R g . For L Ӷ R g , we numerically verify that asymptotically ϳ N 1+2 . For L ӷ R g , we find ϳ N. In addition, we numerically find the scaling function describing crossover between short and long pores. We also show that has a minimum as a function of L for longer chains when the radius of gyration along the pore direction R ʈ Ϸ L. Finally, we demonstrate that the stiffness of the polymer does not change the scaling behavior of translocation dynamics for single-segment dynamics.

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Section: Introductionmentioning

confidence: 99%

Polymer translocation through a nanopore: A two-dimensional Monte Carlo study

Luo

Ala-Nissilä

Ying

2006

The Journal of Chemical Physics

119

144

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“…Electroporation, also called electropermeabilization, is a widely used technique to introduce foreign materials into the cells across the plasmatic membrane by means of pulsed high electric fields [1]. The main distinctive property of the technique is the fact that electroporation is based only on physical phenomena preventing the use of chemical agents.…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, when adherent cells are electroporated a previous trypsinization process is necessary causing additional stress to the cells *This study was supported by the project VALTEC09-1-0061 from Generalitat de Catalunya, ACC1Ó. 1 that may affect both the efficiency and the invasiveness of the operation [4]. As explained in [1] there are several reasons to believe that in situ electroporation is more suitable for high efficacy transfection in adherent cells.…”

Section: Introductionmentioning

confidence: 99%

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Automatic system for electroporation of adherent cells growing in standard multi-well plates

García-Sánchez

Guitart

Rosell

et al. 2012

2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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Abstract-In this study an automatic system is presented to perform electroporation, also known as electropermeabilization, on adherent cells. It is an intention of this system to apply electric field pulses directly to cells growing in standard multiwell plates as a step forward to include this technique in standard laboratory protocols. An interdigitated microelectrode assembly constructed with Printed Circuit Board (PCB) is placed closely above the cell monolayer, and in order to avoid direct contact with cells, small micro-separators were included in the structure. Additionally, distribution of current density was modified by filling the gap between adjacent electrodes with a non conductive material as predicted by electric field simulations. This modification helps to concentrate the electric field intensity in the region where cells are present. The device was tested using C2C12 cell line growing adhered in 24 multiwell plates and fluorescent labeled dextran FD20S as the molecule to be delivered. Successful transfection was observed with minimal invasiveness of the operation reducing the stress caused to cells.

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“…It's causing the cells membrane swelling and broken after than. The main effect as the influence of electric fields given to micro organism cells is for increasing permeability of membrane in this case pressure at the membrane and pores forming [6]. By increasing the electric fields and wave duration it will make the pores will become larger and will create the hole at membrane cells [26].…”

Section: Pef Technology Applied To Apple Juice Pasteurizationmentioning

confidence: 99%