Overview of Electroporation and Electrofusion

Chang, Donald C.; Saunders, James A.; Chassy, Bruce M.; Sowers, Arthur E.

doi:10.1016/b978-0-12-168040-4.50004-3

Cited by 166 publications

(206 citation statements)

References 17 publications

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“…This term referred to the fact that a formation of nanopores was formed in cellular membranes to allow passage of desirable substances. For many years, research was focused on permeabilizing the cell membrane temporarily for applications such as fusion of cells, gene and drug delivery [41,53–64]. This temporary permeabilization, maintaining cell viability, was called `reversible electroporation' (RE).…”

Section: Pfdd Methods and Applicationsmentioning

confidence: 99%

Physically Facilitating drug-delivery Systems

Rodríguez-Dévora

Ambure

Shi

et al. 2011

Therapeutic Delivery

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Facilitated/modulated drug-delivery systems have emerged as a possible solution for delivery of drugs of interest to pre-allocated sites at predetermined doses for predefined periods of time. Over the past decade, the use of different physical methods and mechanisms to mediate drug release and delivery has grown significantly. This emerging area of research has important implications for development of new therapeutic drugs for efficient treatments. This review aims to introduce and describe different modalities of physically facilitating drug-delivery systems that are currently in use for cancer and other diseases therapy. In particular, delivery methods based on ultrasound, electrical, magnetic and photo modulations are highlighted. Current uses and areas of improvement for these different physically facilitating drug-delivery systems are discussed. Furthermore, the main advantages and drawbacks of these technologies reviewed are compared. The review ends with a speculative viewpoint of how research is expected to evolve in the upcoming years.

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Section: Pfdd Methods and Applicationsmentioning

confidence: 99%

Physically Facilitating drug-delivery Systems

Rodríguez-Dévora

Ambure

Shi

et al. 2011

Therapeutic Delivery

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“…[6] For example, the required transformation step limits the library size to approximately 10 10 , which necessitates a substantial labor investment, as detailed by Dower and Cwirla. [7] Furthermore, selection in the context of the host environment cannot be completely avoided, possibly resulting in the loss of potential candidates due to a growth disadvantage or even toxicity in Escherichia coli. Importantly, however, the expression of scFv fragments in the Escherichia coli periplasm allows for rapid assessment of the antigen-binding properties, and the produced antibody can be affinity-purified on immobilized antigen.…”

Section: Introductionmentioning

confidence: 99%

Construction, expression and characterisation of a single chain variable fragment in the Escherichia coli periplasmic that recognise MCF-7 breast cancer cell line

Mahgoub

Bolad

2014

J Can Res Ther

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“…Compared to the chemical delivery strategies, physical approaches grew fast in recent years, benefited from their direct delivery to desired intracellular locations1516171819. Among them, electroporation is often favorable for its balance of simplicity, transfection effectiveness, broad allowance on probe or cell types, and operation convenience202122. In electroporation, short, high-voltage electric pulses are applied to surpass the cell membrane capacitance, making the subjected cells transiently permeable20.…”

mentioning

confidence: 99%

“…Among them, electroporation is often favorable for its balance of simplicity, transfection effectiveness, broad allowance on probe or cell types, and operation convenience202122. In electroporation, short, high-voltage electric pulses are applied to surpass the cell membrane capacitance, making the subjected cells transiently permeable20. It has two active but relatively independent research directions: single cell electroporation (SCE) and bulk electroporation (BE).…”

mentioning

confidence: 99%

“…~54 V across electrodes separated by 2 mm) is enough for successful cell permeabilization. However, the practical pulse strength adopted in most bulk electroporation protocols is 0.5~1.0 kV/cm for mammalian cells and varies with cell type, source, and population202122. The available protocols are established by trial-and-error, instead of equation (1), at a compromise of acceptable transfection efficiency and cell viability.…”

mentioning

confidence: 99%

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Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation

Huang

et al. 2016

Sci Rep

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Electroporation serves as a promising non-viral gene delivery approach, while its current configuration carries several drawbacks associated with high-voltage electrical pulses and heterogeneous treatment on individual cells. Here we developed a new micropillar array electroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes into mammalian cells. By introducing well-patterned micropillar array texture on the electrode surface, the number of pillars each cell faces varies with its plasma membrane surface area, despite their large population and random locations. In this way, cell size specific electroporation is conveniently carried out, contributing to a 2.5~3 fold increase on plasmid DNA transfection and an additional 10–55% transgene knockdown with siRNA probes, respectively. The delivery efficiency varies with the number and size of micropillars as well as their pattern density. As MAE works like many single cell electroporation are carried out in parallel, the electrophysiology response of individual cells is representative, which has potentials to facilitate the tedious, cell-specific protocol screening process in current bulk electroporation (i.e., electroporation to a large population of cells). Its success might promote the wide adoption of electroporation as a safe and effective non-viral gene delivery approach needed in many biological research and clinical treatments.

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Overview of Electroporation and Electrofusion

Cited by 166 publications

References 17 publications

Physically Facilitating drug-delivery Systems

Physically Facilitating drug-delivery Systems

Construction, expression and characterisation of a single chain variable fragment in the Escherichia coli periplasmic that recognise MCF-7 breast cancer cell line

Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation

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