Overview of Fungal Antigens

Drouhet, E.

doi:10.1007/978-1-4613-0773-0_1

Cited by 2 publications

(1 citation statement)

References 120 publications

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“…These assays successfully tested plant tissue samples to detect viruses (Voller et al , ; Clark & Adams, ), bacteria (Nomé et al , ), fungal pathogens and hyperparasites (Casper & Mendgen, ; Johnson et al , ). Nevertheless, pathogen diagnoses in these early tests were generally restricted to genus level, and while the techniques were widely applied with success in plant virus screening (Raju & Olson, ; Burger & Von Wechmar, ), the greater structural complexity of fungal and oomycete pathogens (Drouhet, ) generally resulted in much cross‐reactivity with polyclonal antibodies and the consequentially often poor diagnostic discrimination hindered early commercial applications of immunodiagnostic assays (Mendgen, ; Barker & Pitt, ).…”

Section: Molecular Approaches To Disease Diagnosismentioning

confidence: 99%

Diagnostic tests and their application in the management of soil- and water-borne oomycete pathogen species

Wakeham

Pettitt

2016

Ann Appl Biol

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Oomycete diseases cause significant losses across a broad range of crop and aquaculture commodities worldwide. These losses can be greatly reduced by disease management practices steered by accurate and early diagnoses of pathogen presence. Determinations of disease potential can help guide optimal crop rotation regimes, varietal selections, targeted control measures, harvest timings and crop post-harvest handling. Pathogen detection prior to infection can also reduce the incidence of disease epidemics. Classical methods for the isolation of oomycete pathogens are normally deployed only after disease symptom appearance. These processes are often time consuming, relying on culturing the putative pathogen(s) and the availability of expert taxonomic skills for accurate identification, a situation that frequently results in either delayed application, or routine 'blanket' over-application of control measures. Increasing concerns about pesticides in the environment and the food chain, removal or restriction of their usage combined with rising costs have focussed interest in the development and improvement of disease management systems. To be effective, these require timely, accurate and preferably quantitative diagnoses. A wide range of rapid diagnostic tools, from point of care immunodiagnostic kits to next generation nucleotide sequencing have potential application in oomycete disease management. Here we review currently available as well as promising new technologies in the context of commercial agricultural production systems, considering the impacts of specific biotic and abiotic and other important factors such as speed and ease of access to information and cost effectiveness.

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Section: Molecular Approaches To Disease Diagnosismentioning

confidence: 99%

Diagnostic tests and their application in the management of soil- and water-borne oomycete pathogen species

Wakeham

Pettitt

2016

Ann Appl Biol

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Studies on Fusarium solani

Verma

Gangal

1994

Allergy

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Allergenic cross-reactivity among three Fusarium species (F. solani, F. equiseti, and F. moniliforme) was determined by crossed immunoelectrophoresis (CIE), ELISA inhibition, and immunoprint-inhibition analysis. Inhibiting immunoprint was used to assess the cross-reactivity of individual allergenic proteins in culture filtrate (CF), separated on SDS-PAGE. Dose-response inhibition of band 14 kDa in immunoprints of F. solani CF by antigens of F. solani CF, F. equiseti CF, and F. moniliforme CF demonstrated significant cross-reactivity of allergenic proteins on analysis of densitometric scans. CIE and ELISA inhibition using hyperimmune sera raised in rabbits against F. solani CF antigen also demonstrated shared antigenicity among Fusarium species. Collectively, this study revealed the presence of shared as well as unique antigenic and allergenic determinants in F. solani CF, F. equiseti CF, and F. moniliforme CF antigens.

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Overview of Fungal Antigens

Cited by 2 publications

References 120 publications

Diagnostic tests and their application in the management of soil- and water-borne oomycete pathogen species

Diagnostic tests and their application in the management of soil- and water-borne oomycete pathogen species

Studies on Fusarium solani

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