Overview of Gene Expression Analysis in Gastric Disease Infected with Helicobacter pylori: CLDN1 and MMP9 Could Be Biomarkers for Early Diagnosis of Gastric Cancer

Rivas-Ortiz, Claudia Ivette; Morales-Guerrero, Stephanie Euridice; Ponce-de-León-Rosales, Sergio; Gamboa‐Domínguez, Armando; Rangel‐Escareño, Claudia; Uscanga-Domı́nguez, Luis; Aguilar-Gutiérrez, Germán Rubén; Kershenobich-Stalnikowitz, David; López‐Vidal, Yolanda; Castillo-Rojas, Gonzalo

doi:10.3390/pr10020196

Cited by 2 publications

(1 citation statement)

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“…With the in-depth exploration of the molecular level of GC, researchers have further elucidated the pathogenesis of GC from the aspects of epigenetics and molecular features, and HP-related genes are of great significance in the diagnosis and treatment of GC. Chronic HP infection can develop into chronic atrophic gastritis and GC, and MMP9 is significantly expressed in this process, which is a potential biomarker for the early stage of GC development[ 34 ]. The expression of MMP9 is closely associated with HP infection in GC patients, so the quantification of MMP9 expression level in vivo is beneficial for the diagnosis of HP-infected GC.…”

Section: Discussionmentioning

confidence: 99%

Identification of anti-gastric cancer effects and molecular mechanisms of resveratrol: From network pharmacology and bioinformatics to experimental validation

Ma,

Zhang,

Sun

et al. 2024

World J Gastrointest Oncol

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BACKGROUND Gastric cancer (GC) is one of the most aggressive malignancies with limited therapeutic options and a poor prognosis. Resveratrol, a non-flavonoid polyphenolic compound found in a variety of Chinese medicinal materials, has shown excellent anti-GC effect. However, its exact mechanisms of action in GC have not been clarified. AIM To identify the effects of resveratrol on GC progression and explore the related molecular mechanisms. METHODS Action targets of resveratrol and GC-related targets were screened from public databases. The overlapping targets between the two were confirmed using a Venn diagram, and a “Resveratrol-Target-GC” network was constructed using Cytoscape software version 3.9.1. The protein-protein interaction (PPI) network was constructed using STRING database and core targets were identified by PPI network analysis. The Database for Annotation, Visualization and Integrated Discovery database was used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. A “Target-Pathway” network was created by using Cytoscape 3.9.1. The RNA and protein expression levels of core target genes were observed using the Cancer Genome Atlas and the Human Protein Atlas databases. DriverDBv3 and Timer2.0 databases were used for survival and immune infiltration analysis. Subsequently, the findings were further verified by molecular docking technology and in vitro experiments. RESULTS A total of 378 resveratrol action targets and 2154 GC disease targets were obtained from public databases, and 181 intersection targets between the two were screened by Venn diagram. The top 20 core targets were identified by PPI network analysis of the overlapping targets. GO function analysis mainly involved protein binding, identical protein binding, cytoplasm, nucleus, negative regulation of apoptotic process and response to xenobiotic stimulus. KEGG enrichment analysis suggested that the involved signaling pathways mainly included PI3K-AKT signaling pathway, MAPK signaling pathway, IL-17 signaling pathway, TNF signaling pathway, ErbB signaling pathway, etc . FBJ murine osteosarcoma viral oncogene homolog (FOS) and matrix metallopeptidase 9 (MMP9) were selected by differential expression analysis, and they were closely associated with immune infiltration. Molecular docking results showed that resveratrol docked well with these two targets. Resveratrol treatment arrested the cell cycle at the S phase, induced apoptosis, and weakened viability, migration and invasion in a dose-dependent manner. Furthermore, resveratrol could exhibit anti-GC effect by regulating FOS and MMP9 expression. CONCLUSION The anti-GC effects of resveratrol are related to the inhibition of cell proliferation, migration, invasion and induction of cell cycle arrest and apoptosis by targeting FOS and MMP9.

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Section: Discussionmentioning

confidence: 99%