Overview of
            <i>Cryptosporidium</i>
            Presentations at the 10th International Workshops on Opportunistic Protists

Xiao, Lihua

doi:10.1128/ec.00295-08

Cited by 21 publications

(11 citation statements)

References 51 publications

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“…Cryptosporidiosis is a parasitic zoonosis which can cause sustained, serious and often life-threatening disease in immunosuppressed patients and animals [1]. Cryptosporidium spp.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Wang

Li³

et al. 2013

PLoS ONE

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The objective of this study was to determine the prevalence, species and subtypes of Cryptosporidium infecting yaks in the Qinghai Province of Northwestern China. The prevalence of Cryptosporidium spp. was detected by microscopy and nested-PCR. A total of 586 fecal samples were collected from yaks in 6 counties, of which 142 (24.2%) samples tested positive for Cryptosporidium. The small subunit (SSU) rRNA gene of fifty-five samples were amplified and sequenced successfully and demonstrated that Cryptosporidium bovis (31/55, 56.4%) was the most common species, followed by C. parvum (16/55, 29.1%) and C. ryanae (5/55, 9.0%). Mixed infections of C. parvum and C. bovis (n = 2), C. ryanae and C. bovis (n = 1) were also detected. All three species were found in yaks ranging in age from <1 year, 1–2 years, to >2 years. Cryptosporidium was most commonly detected in spring (28.4%), followed by summer (20.9%), then winter (17.5%). Cryptosporidium parvum positive samples were subtyped using the 60 kDa glycoprotein (gp60) gene. Subtypes IIaA15G2R1 (n = 8), IIaA16G2R1 (n = 2), IIaA14G1R1 (n = 1), IIaA14G2R1 (n = 1) and IIaA16G3R1 (n = 1) were detected. All of these subtypes are zoonotic, and may pose a potential threat to human health.

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“…Cryptosporidiosis is a parasitic zoonosis which can cause sustained, serious and often life-threatening disease in immunosuppressed patients and animals [1]. Cryptosporidium spp.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Wang

Li³

et al. 2013

PLoS ONE

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“…In addition, the risk of infection with both C parvum and C bovis appeared to be lower at higher latitudes, although this finding was not significant. 28 However, a potential source of bias was that conventional sequencing of the 18s rRNA gene does not attain detection of mixed Cryptosporidium infections. tion had a significant effect in the multivariable models among the meteorologic factors.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of factors associated with the risk of infection with Cryptosporidium parvum in dairy calves

Szonyi

Chang²,

Wade³

et al. 2012

ajvr

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“…We restricted the analysis to preweaned calves, because the risk of infection with zoonotic Cryptosporidium has been shown to be limited to this age group, and the majority of Cryptosporidium oocysts shed by preweaned calves is zoonotic Starkey et al 2005;Xiao 2009). Given these findings, modeling the prevalence of C. parvum-like species in preweaned calves provides an accurate estimate of the human health risk posed by dairy cattle regarding zoonotic Cryptosporidium within the watershed.…”

Section: Discussionmentioning

confidence: 99%

Seasonal variation in the prevalence and molecular epidemiology of Cryptosporidium infection in dairy cattle in the New York City Watershed

et al. 2010

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We conducted cross-sectional studies in the New York City Watershed to ensure a valid estimate of the risk associated with Cryptosporidium infection in dairy herds. Our aims were to obtain species-specific estimates of the prevalence of Cryptosporidium in dairy cattle and to investigate seasonal variations in prevalence. We validated our empirical estimates using a Bayesian approach. Samples were collected on 32 study farms, once in each of 3 different seasons using an age-stratified sampling design. The overall prevalence of Cryptosporidium parvum-like species and Cryptosporidium andersoni among the 1911 animals tested by the flotation method was 5% and 1%, respectively. Among preweaned calves (<65 days of age), the prevalence of C. parvum-like species was twice as high in the summer (26%) compared with the winter (11%). Herd prevalence showed the same seasonal trend. Preweaned calves were also shedding C. andersoni at an average intensity of 20 oocysts per gram of feces. We did not detect C. parvum-like oocysts in cattle older than 5 months. Sequencing of a portion of the 18s rRNA gene revealed that in the summer, 42% of the C. parvum-like oocysts shed by preweaned calves were zoonotic, compared with >74% during the rest of the year. Both empirical and stochastic methods revealed a summer peak in the prevalence of C. parvum-like oocysts in preweaned calves. Determining whether seasonal variation in the prevalence and proportion of Cryptosporidium species shed by preweaned calves is due to management practices or ecological factors will have important implications for effective control of this parasite.

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Overview of Cryptosporidium Presentations at the 10th International Workshops on Opportunistic Protists

Cited by 21 publications

References 51 publications

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Evaluation of factors associated with the risk of infection with Cryptosporidium parvum in dairy calves

Seasonal variation in the prevalence and molecular epidemiology of Cryptosporidium infection in dairy cattle in the New York City Watershed

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