Strawberry (Fragraria x ananassa Duch) cv. Tochiotome is nutrient rich. However, it has a short shelf life, especially at room temperature. Coating is one of the methods used to prolong fruit shelf life. Coating materials, such as nano chitosan (NC) and Aloe vera (AV) gel, are edible and safe to consume. The objective was to analyze the interaction between AV gel and NC concentrations and determine the optimal concentration to improve the quality of strawberries at room temperature. This experiment used a factorial randomized block design with treatments of NC (0, 0.5, and 1%) and AV gel (0, 25, and 50%). The results showed an interaction between AV gel and NC concentrations for all variables. The optimum concentrations to improve the fruit quality of strawberries cv. Tochiotome were 25% AV gel with 1% NC, which increased the value by more than twofold compared with the control (no AV gel and NC) until four days after storage. The fruit quality characteristics were fruit hardness (6.57 N), weight loss (1.23%), titratable acidity (0.74 mL), total phenolic (1.79 µg GAE/FW), total flavonoid (29.85 mg QE/g), vitamin C concentration (43.83 mg/100 g), and shelf life (4.66 days). More than 1% NC may enhance quality. In conclusion, AV and NC are a potential treatment for improving the postharvest quality of strawberry.