Overview of miRNA biogenesis and applications in plants

Gangadhar, Baniekal Hiremath; Venkidasamy, Baskar; Samynathan, Ramkumar; Saranya, Balu; Chung, Ill‐Min; Thiruvengadam, Muthu

doi:10.1007/s11756-021-00763-4

Cited by 10 publications

(7 citation statements)

References 189 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR160, which targets auxin response factors ( ARFs ), mainly functions in plant growth, development, and stress responses [ 66 , 67 , 68 ]. Many studies have implicated hormone crosstalk in the fine-tuning of growth development processes, such as auxin–salicylic acid (SA) and auxin–jasmonic acid (JA) crosstalk [ 69 ]. Furthermore, Lin [ 70 ] found that during seed germination, communication between ABA and auxin by miR160-dependent reduction in auxin response factor 10 ( ARF10 ) expression was observed [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant and Its Wild Type

et al. 2022

Genes

View full text Add to dashboard Cite

A complex molecular regulatory network plays an important role in the development and ripening of fruits and leads to significant differences in apparent characteristics. Comparative transcriptome and sRNAome analyses were performed to reveal the regulatory mechanisms of fruit ripening in a spontaneous early-ripening navel orange mutant (‘Ganqi 4’, Citrus sinensis L. Osbeck) and its wild type (‘Newhall’ navel orange) in this study. At the transcript level, a total of 10792 genes were found to be differentially expressed between MT and WT at the four fruit development stages by RNA-Seq. Additionally, a total of 441 differentially expressed miRNAs were found in the four periods, and some of them belong to 15 families. An integrative analysis of the transcriptome and sRNAome data revealed some factors that regulate the mechanisms of formation of early-ripening traits. First, secondary metabolic materials, especially endogenous hormones, carotenoids, cellulose and pectin, obviously changed during fruit ripening in MT and WT. Second, we found a large number of differentially expressed genes (PP2C, SnRK, JAZ, ARF, PG, and PE) involved in plant hormone signal transduction and starch and sucrose metabolism, which suggests the importance of these metabolic pathways during fruit ripening. Third, the expression patterns of several key miRNAs and their target genes during citrus fruit development and ripening stages were examined. csi-miR156, csi-miR160, csi-miR397, csi-miR3954, and miRN106 suppressed specific transcription factors (SPLs, ARFs, NACs, LACs, and TCPs) that are thought to be important regulators involved in citrus fruit development and ripening. In the present study, we analyzed ripening-related regulatory factors from multiple perspectives and provide new insights into the molecular mechanisms that operate in the early-ripening navel orange mutant ‘Ganqi 4’.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant and Its Wild Type

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…Initially, MIR genes are transcribed from gene promoters by RNA polymerase II, producing single-stranded RNAs that form a coiled-hairpin secondary structure due to near-perfect complementary repeat sequences. This process produces RNA duplexes known as primary transcripts (pri-miRNAs) [ 32 ]. The length and structure of these pri-miRNAs vary between miRNAs and typically range between 100 and 400 nucleotides [ 33 , 34 ].…”

Section: Mirna Biogenesis and Its Regulation In Plant Transcriptsmentioning

confidence: 99%

“…After the conversion of pri-miRNA into pre-miRNA, a miRNA/miRNA* duplex consisting of the guide strand (miRNA) and the passenger strand (miRNA*) will be produced through the action of DCL1. DCL1 cleaves the pre-miRNA, which is then methylated at the 3′ ends by sRNA methyltransferase Hua Enhancer 1 (HEN1) to protect it from exonuclease-mediated degradation [ 32 , 41 ]. The methylated miRNA/miRNA* duplex is then transported into the cytoplasm by the plant homolog of cytoplasmic exportin-5 protein, Hasty (HST) [ 42 ].…”

Section: Mirna Biogenesis and Its Regulation In Plant Transcriptsmentioning

confidence: 99%

Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology

Othman

Mustaffa

Che-Othman

et al. 2023

Plants

View full text Add to dashboard Cite

The application of miRNA mimic technology for silencing mature miRNA began in 2007. This technique originated from the discovery of the INDUCED BY PHOSPHATE STARVATION 1 (IPS1) gene, which was found to be a competitive mimic that prevents the cleavage of the targeted mRNA by miRNA inhibition at the post-transcriptional level. To date, various studies have been conducted to understand the molecular mimic mechanism and to improve the efficiency of this technology. As a result, several mimic tools have been developed: target mimicry (TM), short tandem target mimic (STTM), and molecular sponges (SPs). STTM is the most-developed tool due to its stability and effectiveness in decoying miRNA. This review discusses the application of STTM technology on the loss-of-function studies of miRNA and members from diverse plant species. A modified STTM approach for studying the function of miRNA with spatial–temporal expression under the control of specific promoters is further explored. STTM technology will enhance our understanding of the miRNA activity in plant-tissue-specific development and stress responses for applications in improving plant traits via miRNA regulation.

show abstract

“…They are approximately 20–24 nt in length and are derived from longer precursor (pre-miRNA) molecules with stem-loop structures [ 21 ]. In plants, mature miRNAs target mRNA after transcription through gene silencing complexes, resulting in the transcriptional silencing and regulation of target genes [ 22 ]. Plants respond to stress by stimulating signal transduction substances, stress-protective substances, and secondary metabolites [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of action of microRNA166 on nitric oxide in alfalfa (Medicago sativa L.) under drought stress

Wei,

Wang,

Ruan

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Alfalfa is a perennial forage crop of high importance, but its cultivation is often affected by drought stress. Currently, the investigation of drought-related small RNAs is a popular research topic to uncover plant drought resistance mechanisms. Among these small RNAs, microRNA166 (miR166) is associated with drought in numerous plant species. Initial small RNA sequencing studies have shown that miR166 is highly responsive to exogenous nitric oxide (NO) and drought. Therefore, analyzing the expression of Msa-miR166 under nitric oxide and drought treatment is significant. Result Bioinformatics analysis revealed that the miR166 family is widely distributed among plants, ranging from mosses to eudicots, with significant distribution differences between species. The evolutionary degree of Msa-miR166s is highly similar to that of Barrel medic (Medicago truncatula) and Soybean (Glycine max), but significantly different from the model plant Arabidopsis (Arabidopsis thaliana). It is suggested that there are no significant differences in miR166s within the species, and members of Msa-miR166s can form a typical stem-loop. The lowest level of exogenous nitric oxide was observed in Msa-miR166s under drought stress, followed by individual drought, and the highest level was observed after removing endogenous nitric oxide. Conclusion In response to short-term drought, Msa-miR166s down-regulate expression in alfalfa (Medicago sativa L.). Exogenous nitric oxide can reduce the expression of Msa-miR166s in response to short-term drought. These findings suggest that Msa-miR166e-5p is responsive to environmental changes. The expression levels of target genes showed an opposite trend to Msa-miR166s, verifying the accuracy of Degradome sequencing in the early stage. This suggests that alfalfa experiences drought stress when regulated by exogenous nitric oxide, targeting HD ZIP-III, FRI, and CoA ligase genes. Additionally, the expression of Msa-miR166s in response to drought stress varies between leaves and roots, indicating spatiotemporal specificity.

show abstract

Overview of miRNA biogenesis and applications in plants

Cited by 10 publications

References 189 publications

Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant and Its Wild Type

Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant and Its Wild Type

Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology

Mechanism of action of microRNA166 on nitric oxide in alfalfa (Medicago sativa L.) under drought stress

Contact Info

Product

Resources

About