Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied, Thanih; Moore, Eric

doi:10.3390/bios9030102

Cited by 46 publications

(21 citation statements)

References 168 publications

(172 reference statements)

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“…Considering the importance of ALP assay, various methods have been used for evaluation of ALP but researchers still aim to achieve the desired level of accuracy and sensitivity. 6 With the present technique, a quantification could be done with the ALP assay itself.…”

Section: Discussionmentioning

confidence: 99%

A quantitative method to determine osteogenic differentiation aptness of scaffold

Trivedi

Srivastava

Gupta

et al. 2020

Journal of Oral Biology and Craniofacial Research

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Osteogenic differentiation of Mesenchymal stem cells (MSCs) on scaffold is crucial for bone tissue engineering. Alkaline phosphatase (ALP) assay is an important method of assessing osteogenesis. Here, a very simple and innovative procedure is being described for quantification of osteogenic differentiation of MSCs in presence of scaffold using ALP assay. Different concentrations of the scaffold particles with the same number of MSCs were assayed for alkaline phosphatase activity using p-NPP as substrate for ALP activity. G-bone scaffold was used in concentrations of 5, 20, 60 and 100 mg/ml and same number of MSCs were seeded. Any scaffold which can be grind and weighed may be used. It was found that100 mg/ml G-bone graft was most useful for promoting osteogenesis and addition of growth factors further promoted. So, we were able to ascertain the concentration of scaffold which promotes osteogenesis the most.

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Section: Discussionmentioning

confidence: 99%

A quantitative method to determine osteogenic differentiation aptness of scaffold

Trivedi

Srivastava

Gupta

et al. 2020

Journal of Oral Biology and Craniofacial Research

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“…ALP plays a vital role in catalyzing the dephosphorylation of small molecules, proteins, and nucleic acids . Thus, the visualization of ALP activity provides a simple and effective tool for chemical biology and clinical medicine.…”

Section: Resultsmentioning

confidence: 79%

An Activatable AIEgen Probe for High‐Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision

Yao

et al. 2020

Angewandte Chemie

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Monitoring fluctuations in enzyme overexpression facilitates early tumor detection and excision. An AIEgen probe (DQM‐ALP) for the imaging of alkaline phosphatase (ALP) activity was synthesized. The probe consists of a quinoline‐malononitrile (QM) core decorated with hydrophilic phosphate groups as ALP‐recognition units. The rapid liberation of DQM‐OH aggregates in the presence of ALP resulted in aggregation‐induced fluorescence. The up‐regulation of ALP expression in tumor cells was imaged using DQM‐ALP. The probe permeated into 3D cervical and liver tumor spheroids for imaging spatially heterogeneous ALP activity with high spatial resolution on a two‐photon microscopy platform, providing the fluorescence‐guided recognition of sub‐millimeter tumorigenesis. DQM‐ALP enabled differentiation between tumor and normal tissue ex vivo and in vivo, suggesting that the probe may serve as a powerful tool to assist surgeons during tumor resection.

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“…This is in line with the result reported in [27] where it was concluded that turmeric rhizome powder can only be medicinal when it is not consumed in large amount. It was concluded in [36] that turmeric supplement enhance the performance of broilers when used as feed additives in broiler ration at the rate of 0.75% level. In this study also, it can be concluded that turmeric rhizome powder supplemented at concentration lesser than 100g/kg of total recipe could be hepato-, and renal protective especially at 2%.…”

Section: Fig 5 the Effects Of Turmeric Rhizome Powder (Trp) Supplemented Meal At Different Percentages And Standard Drug On Ast Activity mentioning

confidence: 99%

Effects of Turmeric Rhizome Powder Supplemented Diet on Indomethacin-induced Toxicity in Wistar Rats

Oluwafemi

Ajayi

Oseni

et al. 2021

AJBGMB

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Turmeric (Curcuma longa) L rhizome powder (TRP) was commonly reported to have antiulcerogenic and non-toxicity effects. However, the scientific evidence showing the effectiveness of turmeric in the treatment of gastric ulcer and its non-toxicity effect are controversial. The aim of the study was to investigate the effects of different percentages of turmeric rhizome powder supplemented diet on toxicity induced by indomethacin in Wistar rats. This study investigated the effects of TRP formulated diet on the activities of blood enzymes in indomethacin-induced gastric ulcerated Wistar rats. This investigation was carried out through a 28-day experiment using corn-starch flour meal-based diet containing four levels of TRP (1%, 2%, 5% and 10%) as treatments with five replicates in a completely randomized design. The remaining three groups were fed with basal diet, one group received standard drug, another received no treatment but induced while the last group received no treatment and not induced. 35 male Wistar rats weighing 150-200 g were housed in seven cages and received feed and water ad-libitum. At the end of the experiment, all the animals were sacrificed, blood and some organs were collected and evaluated for hepatoxicity and nephrotoxicity induced by indomethacin (60mg/kg bw). Measurements of serum, kidney and liver alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were performed. Statistical evaluation of the results at a p < 0.05 showed significantly elevated values (P ≤ 0.05) of these enzymes in the kidney of rats in groups B, C, E and G when compared with group F. Increase in kidney ALT and AST activities of animals in groups A, C and D was detected in comparison with group F. Furthermore, there was increase in hepatic ALT and ALP activities of animals in groups A, B, C, D and E compared with animals in group F but a significant increase (P ≤ 0.05) in group G in comparison with group F. Likewise, there was significant increase (P ≤ 0.05) in liver AST activities of rats in groups A, B, C, E and G compared with group F, however, insignificant increase (P ≤ 0.05) was observed in animals in group D in comparison with rats in group F. Indomethacin induced rats (group F) showed a significant increase in serum levels of ALT, AST and ALP compared with rats in groups E and G in all the groups but those fed on 1%, 2%, 5% and 10% turmeric supplemented diet showed decrease in comparison with group F. The increased levels of these enzymes in the serum of animals in ulcerogenic group and some in group D (10%TRP group) could be a sign of tissue injury due to relative toxicity of indomethacin induction in animal model and deleterious effect of turmeric rhizome powder at large concentration. However, decrease levels of these enzymes in pretreated groups could indicate the attenuating potential of turmeric at moderate dose against toxicity effect of indomethacin induction. Therefore, turmeric rhizome powder should be consumed with caution and its percentage in the whole recipe should not be up to 10% (100g/kg).

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Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Cited by 46 publications

References 168 publications

A quantitative method to determine osteogenic differentiation aptness of scaffold

A quantitative method to determine osteogenic differentiation aptness of scaffold

An Activatable AIEgen Probe for High‐Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision

Effects of Turmeric Rhizome Powder Supplemented Diet on Indomethacin-induced Toxicity in Wistar Rats

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