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The purpose of research is to study the effect of the combined use of Eimeria vaccine strains on the intestinal mucosa of chickens by histological methods. The object of the study is broiler chickens of the Ross-308 cross, divided into 3 experimental groups: Group 1 (n = 10): on day 14, 1 dose/head was immunized per os. vaccine "Evalon"; group 2 (n = 10): on day 14, 1 dose/head was immunized per os. vaccine "Eymeriavaks 4M"; Group 3 (n = 10): on day 14, they were immunized peros with a mixture of Evalon vaccines, 0.5 doses/head. and "Eymeriavaks 4M" 0.5 doses / goal. On the 28th day, the chickens of these groups per os were re-infested with the second passage of sporulated oocysts at a dose of 0.5 thousand/head, isolated from the litter of a particular group. The study was performed at the Department of Anatomy, Pathological Anatomy and Surgery, Krasnoyarsk State Agrarian University. In chickens immunized with Evalon and Eymeriavax 4M in the monovariant, at 35 days, 20 days after the start of the experiment, no disturbances in the histoarchitectonics of the mucosal, submucosal, muscular and serous layers were detected in the duodenum and jejunum. When stained with the PAS reaction with alcian blue, mucins in Goblet cells were intensively stained. Premature differentiation of crypt cells into Goblet cells was observed. In chickens vaccinated with a combination of vaccines, at 35 days the most pronounced changes associated with chicken eimeriosis were observed in the duodenum: the largest number of macrogamonts and microgamonts, villous infiltration with mononuclear cells. Catarrhal enteritis was characterized by the formation of a large number of goblet exocrinocytes, intensely stained with alcian blue. During artificial invasion of chickens with oocysts of the second passage of Eimeria, after synchronous vaccination with two live anti-Eimeri vaccines, a greater severity of gametogenesis and violations of the histoarchitectonics of the intestinal villi was observed in comparison with the use of anti-Eimeri vaccines in the monovariant.
The purpose of research is to study the effect of the combined use of Eimeria vaccine strains on the intestinal mucosa of chickens by histological methods. The object of the study is broiler chickens of the Ross-308 cross, divided into 3 experimental groups: Group 1 (n = 10): on day 14, 1 dose/head was immunized per os. vaccine "Evalon"; group 2 (n = 10): on day 14, 1 dose/head was immunized per os. vaccine "Eymeriavaks 4M"; Group 3 (n = 10): on day 14, they were immunized peros with a mixture of Evalon vaccines, 0.5 doses/head. and "Eymeriavaks 4M" 0.5 doses / goal. On the 28th day, the chickens of these groups per os were re-infested with the second passage of sporulated oocysts at a dose of 0.5 thousand/head, isolated from the litter of a particular group. The study was performed at the Department of Anatomy, Pathological Anatomy and Surgery, Krasnoyarsk State Agrarian University. In chickens immunized with Evalon and Eymeriavax 4M in the monovariant, at 35 days, 20 days after the start of the experiment, no disturbances in the histoarchitectonics of the mucosal, submucosal, muscular and serous layers were detected in the duodenum and jejunum. When stained with the PAS reaction with alcian blue, mucins in Goblet cells were intensively stained. Premature differentiation of crypt cells into Goblet cells was observed. In chickens vaccinated with a combination of vaccines, at 35 days the most pronounced changes associated with chicken eimeriosis were observed in the duodenum: the largest number of macrogamonts and microgamonts, villous infiltration with mononuclear cells. Catarrhal enteritis was characterized by the formation of a large number of goblet exocrinocytes, intensely stained with alcian blue. During artificial invasion of chickens with oocysts of the second passage of Eimeria, after synchronous vaccination with two live anti-Eimeri vaccines, a greater severity of gametogenesis and violations of the histoarchitectonics of the intestinal villi was observed in comparison with the use of anti-Eimeri vaccines in the monovariant.
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