This work is mainly focused on studying the effects of cathode aeration in a tubular mediator-less microbial fuel cell. COD removal efficiency and the effect of closing the circuit are among other parameters investigated. A new combination of electrodes, i.e., platinumcoated titanium as the cathode and chrome-/vanadiumcoated stainless steel as the anode, is used in this work. Aeration of the cathode chamber is carried out by addition of oxygen, which plays the role of final electron-acceptor terminal. When the cathode chamber is aerated, the maximum achievable voltage and current are 630 mV and 1.06 mA, respectively. When the cathode operates under anaerobic conditions, COD reduced by only 40 % after 90 h, as opposed to 90 % achieved with cathode aeration, in which case more than 36 % of COD is removed in the first 8 h, while the rest of it is eliminated over a much longer period of time (i.e., 82 h). The best curve fitting for COD removal follows a logarithmic pattern, indicating higher removal levels when more substrate is available. Closing the circuit is followed by a plunge in voltage, which is attributable to the ohmic resistance.