Ovicidal and larvicidal activities of some plant extracts against Aedes aegypti L. and Culex quinquefasciatus Say (Diptera: Culicidae)

Munusamy, Rajiv Gandhi; Appadurai, Daniel Reegan; Sivasankaran, Kuppusamy; Paulraj, Michael Gabriel; Ignacimuthu, S.

doi:10.1016/s2222-1808(16)61070-8

Cited by 21 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, in the first hours, the bacteria could be a potential food source but when the eggs hatch, the emerging larvae die because of the larvicidal activity of L. sphaericus . Ovicidal activity has been reported for plants and marine sponge extracts [ 32 , 33 ], but the mode of action of this compounds on eggs has not been described. Since the hatched eggs percentage was high and very similar between the three L. sphaericus strains and the positive control, there is no ovicidal effect against Ae.…”

Section: Resultsmentioning

confidence: 99%

Efficacy of the vegetative cells of Lysinibacillus sphaericus for biological control of insecticide-resistant Aedes aegypti

Rojas-Pinzón

Dussán

2017

Parasites Vectors

View full text Add to dashboard Cite

BackgroundThe control of Aedes aegypti is usually based on chemical insecticides, but the overuse of these compounds has led to increased resistance. The binary toxin produced by Lysinibacillus sphaericus in the final stages of sporulation is used for mosquito control due to its specificity against the culicid larvae; however, it has been proved that Ae. aegypti is refractory for this toxin. Currently, there is no evidence of the use of L. sphaericus vegetative cells for mosquito biocontrol. Therefore, in this study, the vegetative cells of three L. sphaericus strains were assessed against a field-collected Ae. aegypti, resistant to temephos, and the reference Rockefeller strain.ResultsVegetative cells of L. sphaericus 2362, III(3)7 and OT4b.25 produced between 90% and 100% of larvae mortality in the reference Rockefeller strain. Effective concentrations of each L. sphaericus strain for the four larval stages ranged from 1.4 to 2 × 107 CFU/ml. Likewise, a consortium of L. sphaericus assessed against a field-collected Ae. aegypti resistant to temephos and the Rockefeller strain caused 90% of larvae mortality. Concentrations of L. sphaericus consortium that resulted in larvae mortality of field-collected and Rockefeller Ae. aegypti ranged from 1.7 to 2.5 × 107 CFU/ml. The vegetative cells of L. sphaericus have no effect on the Ae. aegypti eggs and pupae.ConclusionsThe vegetative cells of L. sphaericus are effective against Ae. aegypti larvae, meaning that it could be used in the biological control of these mosquito species. Since the L. sphaericus consortium was effective against temephos-resistant Ae. aegypti, vegetative cells could be an alternative to overcome insecticide-resistant populations. Further studies, should be conducted to reveal the mode of action and the toxic principle of L. sphaericus vegetative cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Efficacy of the vegetative cells of Lysinibacillus sphaericus for biological control of insecticide-resistant Aedes aegypti

Rojas-Pinzón

Dussán

2017

Parasites Vectors

View full text Add to dashboard Cite

show abstract

“…The secondary metabolites produced by plants, such as essential oils, alkaloids and phenolics, have been shown to have several pharmaceutical and insecticidal properties [10]. Therefore, these compounds are targeted when evaluating the activities of vegetal extracts, fractions and isolated substances [11,12,13].…”

Section: Introductionmentioning

confidence: 99%

Larvicidal Compounds Extracted from Helicteres velutina K. Schum (Sterculiaceae) Evaluated against Aedes aegypti L.

et al. 2019

View full text Add to dashboard Cite

Helicteres velutina K. Schum (Sterculiaceae), a member of Malvaceae sensu lato, is a Brazilian endemic plant that has been used by the indigenous tribe Pankarare as an insect repellent. A previous study has reported the isolation of terpenoids, flavonoids and pheophytins, in addition to the larvicidal activity of crude H. velutina extracts derived from the aerial components (leaves, branches/twigs, and flowers). The present study reports the biomonitoring of the effects of fractions and isolated compounds derived from H. velutina against A. aegypti fourth instar larvae. A crude ethanol extract was submitted to liquid–liquid extraction with hexane, dichloromethane, ethyl acetate and n-butanol to obtain their respective fractions. Larvicidal evaluations of the fractions were performed, and the hexane and dichloromethane fractions exhibited greater activities than the other fractions, with LC50 (50% lethal concentration) values of 3.88 and 5.80 mg/mL, respectively. The phytochemical study of these fractions resulted in the isolation and identification of 17 compounds. The molecules were subjected to a virtual screening protocol, and five molecules presented potential larvicidal activity after analyses of their applicability domains. When molecular docking was analysed, only three of these compounds showed an ability to bind with sterol carrier protein-2 (1PZ4), a protein found in the larval intestine. The compounds tiliroside and 7,4′-di-O-methyl-8-O-sulphate flavone showed in vitro larvicidal activity, with LC50 values of 0.275 mg/mL after 72 h and 0.182 mg/mL after 24 h of exposure, respectively. This is the first study to demonstrate the larvicidal activity of sulphated flavonoids against A. aegypti. Our results showed that the presence of the OSO3H group attached to C-8 of the flavonoid was crucial to the larvicidal activity. This research supports the traditional use of H. velutina as an alternative insecticide for the control of A. aegypti, which is a vector for severe arboviruses, such as dengue and chikungunya.

show abstract

“…aegypti at low concentrations. Munusamy et al (2016) showed the ovicidal potential of the species belonging to the Asparagaceae family. The authors revealed that the methanolic extract of Rubia cordifolia roots (0.5 mg/mL) inhibits Ae.…”

Section: Discussionmentioning

confidence: 99%

Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae)

et al. 2021

View full text Add to dashboard Cite

Aedes aegypti is a vector of emerging and neglected diseases, such as dengue, chikungunya, and Zika. Helicteres velutina, known as "pitó" in Brazil, is traditionally used as an insect repellent, and several studies have demonstrated its larvicidal activity. The aim of this study was to investigate this species and evaluate its potential ovicidal, pupicidal, adulticidal, and repellent activity. The viability of the eggs was evaluated using different concentrations of the test substances for 25 days. The hexane fraction killed 72.7% of the eggs, while dichloromethane killed 67.7%. The survival of the pupae and adults was verified after 72 h and 48 h, respectively. The LC 50 for the hexane and dichloromethane fractions was 0.12 mg/mL and 8.85 mg/mL for pupae, 8.01 mg/mL and 0.74 mg/mL for adults (tarsal test), and 0.05 mg/mL and 0.23 mg/mL for adults (body test), respectively. Repellency was assessed for 240 min using neonatal Wistar rats on a Y-tube olfactometer. The hexane fraction attracted mosquitoes to the test chamber, while the dichloromethane fraction had a repellent action. The 7,4'-di-O-methyl-8-O-sulfate flavone provides greater repellency, and this finding is similar to the results of the in silico studies that have shown the potential of this substance against adult mosquitoes. This suggests that 7,4'-di-O-methyl-8-O-sulfate flavone may be one of the substances present in the extract from aerial parts of H. velutina that is responsible for the repellent activity mentioned in traditional medicine. These findings provide a better understanding of the insecticidal and repellent activity of the extract, fraction, and compounds isolated from H. velutina against Ae. aegypti, thereby revealing its potential in the development of a more effective botanical insecticide.

show abstract

Ovicidal and larvicidal activities of some plant extracts against Aedes aegypti L. and Culex quinquefasciatus Say (Diptera: Culicidae)

Cited by 21 publications

References 20 publications

Efficacy of the vegetative cells of Lysinibacillus sphaericus for biological control of insecticide-resistant Aedes aegypti

Efficacy of the vegetative cells of Lysinibacillus sphaericus for biological control of insecticide-resistant Aedes aegypti

Larvicidal Compounds Extracted from Helicteres velutina K. Schum (Sterculiaceae) Evaluated against Aedes aegypti L.

Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae)

Contact Info

Product

Resources

About