Oviductal and endometrial mRNA expression of implantation candidate biomarkers during early pregnancy in rabbit

Abstract

SummaryPrenatal losses are a complex problem. Pregnancy requires orchestrated communication between the embryo and the uterus that includes secretions from the embryo to signal pregnancy recognition and secretion and remodelling from the uterine epithelium. Most of these losses are characterized by asynchronization between embryo and uterus. To better understand possible causes, an analysis was conducted of gene expression of a set of transcripts related to maternal recognition and establishment of rabbit pregnancy (uteroglobin, SCGB1A1; integrin α1, ITGA1; interferon-γ, IFNG; vascular endothelial growth factor, VEGF) in oviduct and uterine tissue at 16, 72 or 144 h post-ovulation and insemination. In the oviduct tissue, a significant decrease in the level of SCGB1A1 mRNA expression was observed from 144 h post-ovulation. In the case of ITGA1, the transcript abundance was initially lower, but mRNA expression increased significantly at 72 and 144 h post-ovulation. For IFNG, a huge decrease was observed from 16 to 72 h post-ovulation. Finally, no significant differences were observed in the VEGF transcript. For the endometrium, the results showed a significant decline in the level of SCGB1A1 mRNA expression from 16 to 144 h post-ovulation induction. The highest levels of ITGA1 transcript were detected at 144 h, followed by the 16 h group and lower at 72 h post-ovulation. For IFNG there were no significant differences among post-ovulation induction times. Finally, it was possible to observe that VEGF mRNA abundance was present at low levels at 16 h post-ovulation and remained low at 72 h, but increased at 144 h. The functional significance of these observations may provide new insights into the maternal role in prenatal losses.

Effects of N Acetylcysteine on the Expression of Genes Associated with Reproductive Performance in the Goat Uterus during Early Gestation

“…IGF1 plays crucial roles in the regulation of sexual development and reproduction in mammals [ 58 ]. Numerous studies have shown that members of the integrin family are highly expressed in the gonadal axis of female animals and play an important role in embryonic attachment [ 59 , 60 , 61 ], and the present study showed that the ITGAM , ITGAV , ITGA1 , ITGA5 , ITGB1, and ITGB2 integrin families are highly expressed in the treatment group, which suggests that integrins play an important role in the modulation of reproduction in does. KRAS plays an important role in regulating the implantation process in mouse embryos [ 62 ].…”

Aglepristone Use for Termination of Pregnancy in Rabbits

“…Tavşanlarda düzenli bir östrus döngüsü yoktur [12]. Ovulasyon çiftleşmeye, benzer manipülasyonlara ya da hormon kullanımına bağlıdır [13] [16]. Bu esnada vasküler endotelyal büyüme faktörü salınarak mikrovasküler endotelyal hücre proliferasyonu, embriyogenez, korpus luteum gelişimi ve implantasyonda neovaskülarizasyonla ilişkili hücre göçünü artırır.…”

“…Bu aşamadan sonra serum östrojen seviyesi düşmeye başlayarak [15] bazal seviyede bulunan P4 konsantrasyonu (<2 ng/mL) [13] yükselmeye başlar ve gebelik süresince yüksek seyreder. Gebeliğin ilk günlerinde uterus epitelyumunda trofoblast odakcıkları olarak bilinen blastosist bağlanma noktalarının oluşması, histotrof uterus sekresyonundan uteroglobin (SCGB1A1) salınımı artmaya başlaması gebelik için uygun ortamı hazırlar [16]. Bu esnada vasküler endotelyal büyüme faktörü salınarak mikrovasküler endotelyal hücre proliferasyonu, embriyogenez, korpus luteum gelişimi ve implantasyonda neovaskülarizasyonla ilişkili hücre göçünü artırır.…”

“…Bu esnada vasküler endotelyal büyüme faktörü salınarak mikrovasküler endotelyal hücre proliferasyonu, embriyogenez, korpus luteum gelişimi ve implantasyonda neovaskülarizasyonla ilişkili hücre göçünü artırır. Ayrıca interferon-γ salınımı ile gebeliğin maternal kabulü sağlanır [16]. Gebelik teşhisi real time ultrasonografi ile gebeliğin 5-6. gününden sonra mümkün olabilmekle beraber ortalama 7.8 günde fetüslerin kalp atımlarının net bir şekilde tespit edildiği bildirilmiştir [17].…”

Candidate genes associated with reproductive traits in rabbits

“…The product of this gene is oviductal glycoprotein 1 (OVGP1), which is one of the major components of oviductal fluid (Yu et al, 2019 ). This fluid provides the ideal environment, including temperature, pH, and oxygen tension, and also controls early pregnancy (Killian, 2004 ; Saeed et al, 2015 ).…”