Ovine adenovirus (OAV287) lacks a virus-associated RNA gene.

Venktesh, A.; Watt, Fujiko; Xu, Zheng; Both, Gerald W.

doi:10.1099/0022-1317-79-3-509

Cited by 16 publications

(9 citation statements)

References 36 publications

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“…Given the nature of ITR sequences, this implies that the left ITR may contain the LHP1 promoter and that it may also function immediately after infection to generate LH1 and LH2 gene products 12 , 15 . Lastly, the OAdV7 lacks a VA RNA gene, although such a gene is present at the right end of DAdV1 13 , 18 . One or two VA RNA genes are commonly found in mastadenoviruses 19 .…”

Section: Transcripts and Promotersmentioning

confidence: 99%

Ovine atadenovirus: a review of its biology, biosafety profile and application as a gene delivery vector

Both

2004

Immunol Cell Biol

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SummaryThe ovine adenovirus isolate OAdV287 is the prototype of the newly recognized genus of atadenoviruses. Although not as well studied as human mastadenoviruses, a substantial amount of work has now been carried out with this virus and an understanding of its interesting and unique properties is beginning to emerge. In this article the biology and biosafety profile of the virus is reviewed. This knowledge underpins the exploitation of the virus as a gene delivery vector. Its potential as a vaccine vector and its application to the treatment of prostate cancer is summarized and discussed.

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Section: Transcripts and Promotersmentioning

confidence: 99%

Ovine atadenovirus: a review of its biology, biosafety profile and application as a gene delivery vector

Both

2004

Immunol Cell Biol

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“…On the basis of limited sequence similarity to FAdV-1, a candidate VA RNA gene was identified in the DAdV-1 genome, overlapping the 39 end of ORF4 (Hess et al, 1997). This region is absent from the other two atadenoviruses sequenced, and functional investigations have failed to detect a VA RNA in OAdV-7 (Venktesh et al, 1998). If the identified DAdV-1 VA RNA gene is genuine, its evolution might have been independent or might have involved transfer to or from an aviadenovirus.…”

Section: Genus-specific Genesmentioning

confidence: 99%

Genetic content and evolution of adenoviruses

Davison¹,

Benkő

Harrach

2003

Journal of General Virology

548

458

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This review provides an update of the genetic content, phylogeny and evolution of the family Adenoviridae. An appraisal of the condition of adenovirus genomics highlights the need to ensure that public sequence information is interpreted accurately. To this end, all complete genome sequences available have been reannotated. Adenoviruses fall into four recognized genera, plus possibly a fifth, which have apparently evolved with their vertebrate hosts, but have also engaged in a number of interspecies transmission events. Genes inherited by all modern adenoviruses from their common ancestor are located centrally in the genome and are involved in replication and packaging of viral DNA and formation and structure of the virion. Additional niche-specific genes have accumulated in each lineage, mostly near the genome termini. Capture and duplication of genes in the setting of a 'leader-exon structure', which results from widespread use of splicing, appear to have been central to adenovirus evolution. The antiquity of the pre-vertebrate lineages that ultimately gave rise to the Adenoviridae is illustrated by morphological similarities between adenoviruses and bacteriophages, and by use of a protein-primed DNA replication strategy by adenoviruses, certain bacteria and bacteriophages, and linear plasmids of fungi and plants. INTRODUCTIONThe purpose of this review is to provide a comprehensive update of the genetic content, phylogeny and evolution of the family Adenoviridae, whose members infect hosts throughout the vertebrates (Russell & Benkó´, 1999). This area has frequently taken a back seat to studies of the interactions of selected human adenovirus proteins with cellular processes and, more recently, to the use of adenoviruses as vectors. Our aim is to redress this imbalance by bringing together published information and by offering fresh insights into the genomics of the family as a whole. It is not our purpose to deal in broad scope with other areas, such as the expression, functions and interactions of adenovirus gene products and the ways in which host defences are manipulated during the infectious cycle. These have been reviewed recently by Russell (2000) and Shenk (2001) for human adenoviruses. Phylogeny, classification and genetic organizationMembers of the family Adenoviridae are non-enveloped, icosahedral viruses that replicate in the nucleus. Their linear, double-stranded DNA molecules are 26-45 kbp in size and rank as medium-sized among the DNA viruses. The genomes are characterized by an inverted terminal repeat (ITR) ranging in size from 36 to over 200 bp, and the 59 ends are linked to a terminal protein (TP). Phylogenetic relationships among a large number of adenoviruses infecting vertebrates from fish to humans are shown in Fig. 1. The major clades (groups of viruses sharing a common ancestor) correspond to the four accepted genera plus a fifth genus that is likely to be added in due course (Benkó´et al., 2002;. Two genera (Mastadenovirus and Aviadenovirus) originate from mammals or birds, respective...

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“…OAdV is distinguished from members of the mast-and aviadenoviruses by a different genome organization and by the fact that many open reading frames show no detectable homology to those of any other adenoviruses (53)(54)(55)(56). Because the biology of OAdV is not well understood, the outcome of infecting nonovine, particularly human, cells with OAdV is of interest.…”

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Biology of Ovine Adenovirus Infection of Nonpermissive Cells

Kümin¹,

Hofmann²,

Rudolph

et al. 2002

J Virol

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Nonhuman adenoviruses, including those of the genus Atadenovirus, have the potential to serve as vectors for vaccine and gene therapy applications in humans, since they are resistant to preexisting immunity induced by human adenoviruses in the majority of the population. In this study, we elucidate the outcome of infection by ovine adenovirus type 7 isolate 287 (OAdV) of several nonovine cell types. We show here that OAdV infects a wide range of nonovine cells but is unable to complete its replication cycle in any of them. In nonovine, nonfibroblast cells, viral replication is blocked at an early stage before the onset of, or early in, DNA replication. Some fibroblasts, on the other hand, allow viral DNA replication but block virus production at a later stage during or after the translation of late viral proteins. Late viral proteins are expressed in cells where viral DNA replication takes place, albeit at a reduced level. Significantly, late proteins are not properly processed, and their cellular distribution differs from that observed in infected ovine cells. Thus, our results clearly show that OAdV infection of all nonovine cells tested is abortive even if significant viral DNA replication occurs. These findings have significant positive implications with respect to the safety of the vector system and its future use in humans.Human adenoviruses have been widely studied over the last 4 decades, and the processes involved in infection and replication have been largely characterized. Abortive infection by human adenoviruses has been investigated in human and several nonhuman cell culture systems (reviewed in reference 35). These studies have provided a better understanding of the molecular biology of human adenoviruses and of events such as the integration of foreign DNA into mammalian genomes and the initiation of malignant transformation by DNA tumor viruses. However, the biology of human adenovirus infection in heterologous cells differs widely for various genotypes, even in the same cell line. For example, baby hamster kidney cells (BHK-21) are semipermissive for adenovirus type 2 (Ad2) and Ad5 replication but block infection with Ad3 and Ad12 at an early step of the viral life cycle (13). The early block in Ad12 replication can be abrogated by constitutive expression of E1 genes from Ad2, -

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Ovine adenovirus (OAV287) lacks a virus-associated RNA gene.

Cited by 16 publications

References 36 publications

Ovine atadenovirus: a review of its biology, biosafety profile and application as a gene delivery vector

Ovine atadenovirus: a review of its biology, biosafety profile and application as a gene delivery vector

Genetic content and evolution of adenoviruses

Biology of Ovine Adenovirus Infection of Nonpermissive Cells

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