Ovine CD14- an Immune Response Gene Has a Role Against Gastrointestinal Nematode Haemonchus contortus—A Novel Report

Rawat, Kavita; Pal, Aruna; Banerjee, Sandipan; Pal, Abantika; Mandal, S. C.; Batabyal, Subhasis

doi:10.3389/fimmu.2021.664877

Cited by 12 publications

(13 citation statements)

References 88 publications

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“…The current study aims to explore the host immunity against dreadly gastrointestinal parasite Haemonchus contortus in sheep model. Although this is a less explored field, we have earlier documented RIGI (well known antiviral molecule) and CD14 (well known antibacterial molecule) to have a potent role in providing host immunity against Haemonchus contortus (Banerjee et al , 2021, Rawat et al ., 2021). In this study, we had explored IL6 and IL10 as potent immune response genes for host immunity against Haemonchus contortus.…”

Section: Discussionmentioning

confidence: 99%

Role of IL-6 and IL10 immune response genes against nematodeHaemonchus contortusin ovis aries- a novel report

Rawat,

Pal,

Banerjee

et al. 2023

Preprint

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Haemonchus contortus is one of the most deadly parasites affecting sheep industry causing heavy economic loss. In our earlier study, we have reported RIGI, an earlier established antiviral molecule and CD14 (an earlier established antibacterial molecule) to have potential role in antiparasitic immunity for the first time. In this current study, we aim to clone and sequence the IL-6 and IL10 genes in sheep (Ovis aries) and utilized bioinformatics methods to analyze and compare the functional and structural domains of peptides produced from IL6 and IL10. Furthermore, the study explored these domains in three-dimensional structures. The findings of this study provide molecular characterization of IL-6 and IL10 in sheep, which is essential for advancing our understanding of functional immune responses in this animal. We report IL6 and IL10 to have potential antiparasitic role against H. contortus for first time. The production of these molecules opens up possibilities for its potential use as recombinant proteins to manipulate the immune response. Evaluating its value in vaccine research and gaining a broader understanding of its role in immune responses becomes feasible with the availability of IL-6 and IL10. Moreover, the differential mRNA expression levels of IL-6, IL10 between healthy and diseased sheep indicate its crucial role in maintaining homeostasis and mounting immune responses. IL10 acts as receptor for H. contortus. The identification of this immune-responsive gene also offers new avenues for investigating long-term resistance to H. contortus infection in sheep.

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Section: Discussionmentioning

confidence: 99%

Role of IL-6 and IL10 immune response genes against nematodeHaemonchus contortusin ovis aries- a novel report

Rawat,

Pal,

Banerjee

et al. 2023

Preprint

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“…The samples of ovary (n=6) were collected from each group of non-layer (sexually immature or infertile duck, Group I) and highest laying duck (Group II) were collected. The total RNA was isolated from the ovary of Duck by Trizol method and was further used for cDNA synthesis [38][39][40][41][42] .…”

Section: Materials and Methodmentioning

confidence: 99%

“…Each sample was run in duplicate. Analysis of real-time PCR (qRT-PCR) was performed by delta-delta-Ct (ΔΔCt) method 31,2,4,38,39,42 .…”

Section: Methodsmentioning

confidence: 99%

Genomics for reproduction in Anas platyrynchos-a novel report

Debnath

Pal

Chakraborty

et al. 2022

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Anas platyrynchos (ducks) are reared mostly for egg, which are very nutritious, that fetch better prices, however duck meat possess rich nutrient content. They possess the unique characteristics of disease resistance to the common avian diseases, even asymptomatic to avian influenza, with a scopeto evolve as one of the best poultry species The major limitation encountered is the lowered average egg production as well as higher age at first egg (an indicator for sexual maturity) for the indigenous ducks compared to that of exotic ones. In this current study, we attempt to explore the genes responsible for duck reproduction in terms of sexual maturity,egg production and fertility of the ducks . We had compared the genomic constitution for the Bengal duck with highest egg production with that of non-layer (infertile or sexually immature) ducks. We characterized the genes in indigenous ducks from ovarian tissues, identified important domains for characterized genes for the first time, and studied differential mRNA expression profiling for these genes with respect to layer and non-layer groups. Upregulation was observed for ESR2, DIAPH2, KMT2E, ASCF2 genes for Bengal duck in highest egg producing duck in comparison to non-layer duck, whereas downregulation was observed for KSR1, A2M, BMPR1B, ACVR1. In the next step, we explored the association with the genes which were actually responsible for egg production. Thus, duck may be utilized as a model for studying the molecular aspect of reproduction. Genes upregulated may be utilized for knock in of gene, whereas down regulated genes may be knocked out or knocked down through gene editing technologies for the improvement of reproductive performance of the duck in future. Molecular biomarkers may be developed with these genes for early selection of better reproducing ducks at day of hatch or even earlier.

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“…We refer them as immune response genes, generally considered as nuclear genes regulating PRR, proinflammatory and anti inflammatory cytokines. In our lab,we have reported the role of certain set of nuclear genes in controlling disease resistance or animal health in sheep 1,2 , cattle 3 , buffalo 4 , goat 5,6 , chicken 7 and duck 8,9 model 10 . Simultaneously, we have also reported the role of mitochondrial genes in controlling health and disease resistance 11 , and even reproduction 12 .…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial genes affect the immune response against bacterial infection (Duck Pasteurellosis) through a cascade of mechanisms mediated by nuclear immune genes-a cross talk with nuclear and mitochondrial gene

Chakraborty,

Pal,

Debnath

et al. 2023

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Mitochondria possess 37 genes of its own, helpful for carrying out various functions including oxidative phosphorylation. But the mitochondria is neither independent in terms of its structure, nor its function, it depend upon nuclear gene for its functionality and replication. Our lab has earlier reported the role of mitochondrial genome in controlling health and reproduction in animal model. Reports also indicate that mitochondria have role in immune response against bacterial infection. In our current study, we depict the role of two polypeptide coding mitochondrial gene as cytochrome B and cytochrome C in providing host immunity against bacterial disease (Duck Pasteurellosis). We further observe that the mechanism was governed by cascade of mechanisms mediated through nuclear genes NRLP3, IL18 and Sting. In the first phase of the study, we have characterized Cytochrome B and Cytochrome C genes from Bengal duck and quantified through the assesment of the expression profiling with respect to healthy and Pasteurella infected ducks following a natural challenge with Pasteurella multocida. In the next phase of our study, we have characterized the above nuclear genes in Bengal duck and attempted to correlate the expression with mitochondrial genes. We attempted to explore the mechanism of pathway how mitochondrial DNA triggers the nuclear immune response leading to destruction of bacterial pathogen. Molecular docking revealed how IL18 directly binds with the pathogen. This is the first report in animal model.

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Ovine CD14- an Immune Response Gene Has a Role Against Gastrointestinal Nematode Haemonchus contortus—A Novel Report

Cited by 12 publications

References 88 publications

Role of IL-6 and IL10 immune response genes against nematodeHaemonchus contortusin ovis aries- a novel report

Role of IL-6 and IL10 immune response genes against nematodeHaemonchus contortusin ovis aries- a novel report

Genomics for reproduction in Anas platyrynchos-a novel report

Mitochondrial genes affect the immune response against bacterial infection (Duck Pasteurellosis) through a cascade of mechanisms mediated by nuclear immune genes-a cross talk with nuclear and mitochondrial gene

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