Ovine enzootic abortion (OEA): Antibody response in vaccinated sheep compared to naturally infected sheep

Borel, Nicole; Sachse, Konrad; Rassbach, Astrid; Bruckner, L; Vretou, Evangelia; Psarrou, Evgenia; Pospischil, Andreas

doi:10.1007/s11259-005-0844-0

Cited by 18 publications

(21 citation statements)

References 12 publications

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“…However, the boost in the serological response detected by the ELISA in both vaccinated groups (Figure 1) highlights the immunogenicity of C. abortus -inactivated vaccines, evident between 29 and 41 d.p.v., as previously reported (40–42) [showing group SV a higher response than group DV as observed in Wilsmore et al (3)], although different inactivated vaccines have been also reported to induce a scarce (43) or null (4) serological response.…”

Section: Discussionsupporting

confidence: 81%

Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally

et al. 2016

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Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition to reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help to control the disease but variable efficacy values have been described, possibly related with factors associated with the host, the vaccine, the parameter used for efficacy determination, and the challenge conditions. In this context, we evaluated the efficacy of an inactivated standard commercial vaccine and a 1/2 diluted dose in pregnant sheep challenged with C. abortus by examining multiple indicators of vaccine effect (including incidence of reproductive failures, bacterial excretion, and evolution of weight gain of viable lambs during the first month of life). Three groups of ewes [control non-vaccinated, C (n = 18); vaccinated with standard dose, SV (n = 16); and vaccinated with 1/2 dose, DV (n = 17)], were challenged approximately 90 days post-mating and tested using direct PCR (tissue samples and vaginal swabs) and ELISA (serum) until 31 days post-reproductive outcome. There were not significant differences in the proportions of reproductive failures or bacterial shedding after birth/abortion regardless the vaccination protocol. However, a beneficial effect of vaccination on offspring growth was detected in both vaccinated groups compared with the controls, with a mean increase in weight measured at 30 days of life of 1.5 and 2.5 kg (p = 0.056) and an increase in the geometric mean of the daily gain of 8.4 and 9.7% in lambs born from DV and SV ewes compared with controls, respectively. Our results demonstrate the effect of an inactivated vaccine in the development of the offspring of C. abortus-infected ewes at a standard and a diluted dose, an interesting finding given the difficulty in achieving sufficient antigen concentration in the production of enzootic abortion of ewes-commercial vaccines.

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Section: Discussionsupporting

confidence: 81%

Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally

et al. 2016

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“…It is well known that PPRV can infect a multitude of host cells, such as sheep kidney epithelial cells [45] (PO cell line) and goat peripheral blood mononuclear cells (PBMCs) [46]. Throughout our study, caprine endometrial epithelial cells were used for the experiments, and we showed that PPRV can successfully replicate in caprine EECs, further confirming the clinical phenomenon of abortions in PPRV-infected goats [3,4]; these data thus provide a theoretical basis for the replication mechanism of PPRV. Currently, increasing evidence suggests that several genetic connections participate in crosstalk between autophagy and apoptosis [47].…”

Section: Discussionsupporting

confidence: 78%

“…PPR is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats that is associated with high morbidity and mortality. Clinically, the disease is characterized by a high fever, conjunctivitis, oculo-nasal discharge, necrotizing and erosive stomatitis, and diarrhoea [2]; notably, PPRV infection often causes foetal mummification, abortions late in pregnancy, and the birth of dead lambs or weak lambs that die within a couple of days [3,4]. The PPRV genome of approximately 16 kilobases (kb) encodes six structural proteins, namely, the nucleocapsid (N), phospho (P), matrix (M), fusion (F), haemagglutinin (H) and large (L) proteins in the 3′ to 5′ direction (3′-N-P-M-F-H-L-5′); two non-structural proteins (C/V) are also encoded due to the RNA editing of the phosphoprotein gene.…”

Section: Introductionmentioning

confidence: 99%

Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro

Yang

Xue

et al. 2018

Virulence

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Peste des petits ruminants (PPR) is an acute and highly contagious disease in small ruminants that causes significant economic losses in developing countries. An increasing number of studies have demonstrated that both autophagy and apoptosis are important cellular mechanisms for maintaining homeostasis, and they participate in the host response to pathogens. However, the crosstalk between apoptosis and autophagy in host cells during PPRV infection has not been clarified. In this study, autophagy was induced upon virus infection in caprine endometrial epithelial cells (EECs), as determined by the appearance of double- and single-membrane autophagy-like vesicles, LC3-I/LC3-II conversion, and p62 degradation. We also found that PPRV infection triggered a complete autophagic response, most likely mediated by the non-structural protein C and nucleoprotein N. Moreover, our results suggest that autophagy not only promotes the replication of PPRV in EECs but also provides a potential mechanism for inhibiting PPRV-induced apoptosis. Inhibiting autophagosome formation by wortmannin and knocking down the essential autophagic proteins Beclin-1 and ATG7 induces caspase-dependent apoptosis in EECs in PPRV infection. However, inhibiting autophagosome and lysosome fusion by NH4Cl and chloroquine did not increase the number of apoptotic cells. Collectively, these data are the first to indicate that PPRV-induced autophagy inhibits caspase-dependent apoptosis and thus contributes to the enhancement of viral replication and maturity in host cells.

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“…Infection causing foetal mummification, abortion late in the pregnancy, or the birth of dead or weak lambs that die within a couple of days, abortions may also occur in about 30-60% of small ruminants is being confused with ovine chlamydophilosis in which the abortion is an important cause of abortion in many sheep-rearing areas of the world (Borel et al 2005) but as we tested the serum samples from the aborted dams and were found positive for antibodies against PPR so it is confirmed that the cause of abortions was PPR.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and mortality rate of peste des petitis ruminant (ppr): possible association with abortion in goat

Abubakar¹,

Ali²,

Khan

2007

Trop Anim Health Prod

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Present study was designed to investigate the prevalence and mortality (%) caused by Peste des Petitis Ruminant (PPR) and its possible association with abortion in goat flocks at different areas of Pakistan. A total of 140 animals were samples in the population of 650 which was having 185 deaths (Mortality rate = 28 %) from three different regions of the country. There were 58 abortions in the 140 pregnant goats of above said population One hundred & ten (110) serum samples from diseased, recovered and apparently healthy animals were tested for the presence of PPR antibodies by competitive ELISA (c ELISA). Eighty-four (84) animals were positive for PPR antibodies whereas in apparently healthy adult goats in the same flock, no PPR antibodies were detected. Twenty-four (24) tissue samples collected from the dead animals and six samples from aborted fetus were tested for the presence of PPR antigen by Immuno-capture ELISA (Ic ELISA). Nineteen (19) out of thirty (30) organ samples mainly from lung, spleen, lymph node were found positive for PPR antigen but negative from lungs of aborted fetus. There was a high rate of abortions (28-45%) in each of the outbreak and it was highest in the outbreak of Golra Sharif, Islamabad (No. = 21 in total population of 100). As the serum samples from the aborted dams were found positive for PPR antibodies so the study provides the possible association of mortality and prevalence of PPR disease with high rate of abortions in goat.

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Ovine enzootic abortion (OEA): Antibody response in vaccinated sheep compared to naturally infected sheep

Cited by 18 publications

References 12 publications

Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally

Effect of Preventive Chlamydia abortus Vaccination in Offspring Development in Sheep Challenged Experimentally

Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro

Prevalence and mortality rate of peste des petitis ruminant (ppr): possible association with abortion in goat

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