Ovotransferrin alleviated acute gastric mucosal injury in BALB/c mice caused by ethanol

Huang, Yan; Chen, Shuping; Yao, Yao; Wu, Na; Xu, Mingsheng; Du, Hongwu; Zhao, Yan; Tu, Yonggang

doi:10.1039/d2fo02364d

Cited by 15 publications

(3 citation statements)

References 58 publications

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“…In recent years, hydrolysates of major egg proteins like ovalbumin, ovotransferrin, and lysozyme have been reported to have antioxidant properties. [45][46][47] However, for ovomucoid, research focused on its allergenicity, and seldom any attention has been paid to its antioxidant activity. This work demonstrates for the first time that after unfolding, glycation and simulated digestion, the ovomucoid peptides can possess increased antioxidant activity.…”

Section: Papermentioning

confidence: 99%

Effects of unfolding treatment assisted glycation on the IgE/IgG binding capacity and antioxidant activity of ovomucoid

Xia,

Li,

Liang

et al. 2024

Food Funct.

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Section: Papermentioning

confidence: 99%

Effects of unfolding treatment assisted glycation on the IgE/IgG binding capacity and antioxidant activity of ovomucoid

Xia,

Li,

Liang

et al. 2024

Food Funct.

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“…After the final gavage, the mice were fasted for 24 h to ensure complete digestion and absorption of gastric contents, with free access to water during this period. Except for the CK group, all other groups were given 75% ethanol (0.1 mL/20 g) by gavage to induce acute gastric mucosal injury, while the CK group received an equivalent amount of distilled water [22]. One hour after the gavage, the mice were euthanized by dislocating the cervical spine, and organs (liver, kidney, spleen, gastric) and serum were rapidly collected after enucleating the eyeballs and drawing blood from the neck dislocation site.…”

Section: Animals and Groupsmentioning

confidence: 99%

The Gastroprotective Effect of Walnut Peptides: Mechanisms and Impact on Ethanol-Induced Acute Gastric Mucosal Injury in Mice

Yuan,

Wang,

Wang

et al. 2023

Nutrients

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The objective of this research was to explore the protective impact of walnut peptides (WP) against ethanol-induced acute gastric mucosal injury in mice and to investigate the underlying defense mechanisms. Sixty male BALB-c mice were divided into five groups, and they were orally administered distilled water, walnut peptides (200 and 400 mg/kg bw), and omeprazole (20 mg/kg bw) for 24 days. Acute gastric mucosal injury was then induced with 75% ethanol in all groups of mice except the blank control group. Walnut peptides had significant protective and restorative effects on tissue indices of ethanol-induced gastric mucosal damage, with potential gastric anti-ulcer effects. Walnut peptides significantly inhibited the excessive accumulation of alanine aminotransferase (ALT), aspartate transferase (AST), and malondialdehyde (MDA), while promoting the expression of reduced glutathione (GSH), total antioxidant capacity (T-AOC), glutathione disulfide (GSSG), and mouse epidermal growth factor (EGF). Furthermore, the Western blot analysis results revealed that walnut peptides significantly upregulated the expression of HO-1 and NQO1 proteins in the Nrf2 signaling pathway. The defensive impact of walnut peptides on the gastric mucosa may be achieved by mitigating the excessive generation of lipid peroxides and by boosting cellular antioxidant activity.

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“…Our previous studies have found that OVT possesses antiinflammatory, antioxidant, and immunomodulatory activities. 22,23 However, the anti-inflammatory activity and mechanism of the OVT in gastric mucosa are still unclear. Additionally, the human normal gastric epithelial cell (GES-1) line immortalized by simian virus 40 (SV40) can mimic the primary gastric epithelium of adults.…”

Section: Introductionmentioning

confidence: 99%

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Huang

Chen

Yao

et al. 2023

J. Agric. Food Chem.

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Ovotransferrin (OVT) has been confirmed to have anti-inflammatory activity. However, its effect and mechanism on gastric inflammation are unclear. In this study, the effect and mechanism of the OVT on the tumor necrosis factor-α (TNF-α) induced inflammatory response in gastric epithelial cells (GES-1) were investigated. The enzyme linked immunosorbent assay (ELISA) was used to determine the levels of inflammation cytokines, followed by RNA sequencing to explore the potential pathways of its anti-inflammatory effect, and then it was validated by Western blotting and pathways inhibitors. Results showed that the OVT at concentrations of 50–400 μg/mL displayed nontoxicity against GES-1 cells. Additionally, 100 μg/mL of OVT obviously reduced the secretion of interleukin (IL)-8, IL-6, and TNF-α by 63.02% (630.09/1703.98), 35.53% (935.81/1451.43), and 36.19% (964.60/1511.63), respectively. The results of RNA sequencing exhibited that the OVT significantly influences the activation of mitogen-activated protein kinase (MAPK) and the nuclear factor kappa-light-chain enhancer of activated B cell (NF-κB) pathways, which was verified by the levels of p-IKK, p-IκB, p-P65, p-ERK, p-JNK, and p-P38 protein. IL-8 contents released by GES-1 cells after incubation with inhibitors of NF-κB and MAPK pathways further confirmed that OVT hindered activation of these two pathways. Collectively, these results suggested that OVT was a natural protein with the potential to treat gastric inflammation.

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Ovotransferrin alleviated acute gastric mucosal injury in BALB/c mice caused by ethanol

Abstract: Acute gastric mucosal injury is a common gastrointestinal disorder, which influences patients’ life quality.

Cited by 15 publications

References 58 publications

Effects of unfolding treatment assisted glycation on the IgE/IgG binding capacity and antioxidant activity of ovomucoid

Effects of unfolding treatment assisted glycation on the IgE/IgG binding capacity and antioxidant activity of ovomucoid

The Gastroprotective Effect of Walnut Peptides: Mechanisms and Impact on Ethanol-Induced Acute Gastric Mucosal Injury in Mice

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

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