Ovulation and embryo survival rates and plasma progesterone concentrations of prolific ewes treated with PMSG

Rhind, S. M.; Robinson, John J.; Fraser, C.; McHattie, I.

doi:10.1530/jrf.0.0580139

Cited by 22 publications

(7 citation statements)

References 15 publications

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“…On the other hand, Finnsheep (63%) and F1 cross (48%) had a remarkably low embryo survival rate. While these findings are based on fewer animals, the results are in agreement with earlier studies [39,40] where typically higher litter size is associated with higher embryo mortality and vice versa. It would be of great interest to determine if productivity follows the same pattern in F2 (i.e., F1 × F1) crosses, backcrosses and presumably also in a reciprocal cross.…”

Section: Phenotypic Observationssupporting

confidence: 92%

Gene Expression Profiling of Corpus luteum Reveals Important Insights about Early Pregnancy in Domestic Sheep

Pokharel

Peippo

Weldenegodguad

et al. 2020

Genes

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The majority of pregnancy loss in ruminants occurs during the preimplantation stage, which is thus the most critical period determining reproductive success. Here, we performed a comparative transcriptome study by sequencing total mRNA from corpus luteum (CL) collected during the preimplantation stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287 genes were expressed in our data. Highly expressed autosomal genes in the CL were associated with biological processes such as progesterone formation (STAR, CYP11A1, and HSD3B1) and embryo implantation (e.g., TIMP1, TIMP2 and TCTP). Among the list of differentially expressed genes, sialic acid-binding immunoglobulin (Ig)-like lectins (SIGLEC3, SIGLEC14, SIGLEC8), ribosomal proteins (RPL17, RPL34, RPS3A, MRPS33) and chemokines (CCL5, CCL24, CXCL13, CXCL9) were upregulated in Finnsheep, while four multidrug resistance-associated proteins (MRPs) were upregulated in Texel ewes. A total of 17 known genes and two uncharacterized non-coding RNAs (ncRNAs) were differentially expressed in breed-wise comparisons owing to the flushing diet effect. The significantly upregulated TXNL1 gene indicated potential for embryonic diapause in Finnsheep and F1. Moreover, we report, for the first time in any species, several genes that are active in the CL during early pregnancy (including TXNL1, SIGLEC14, SIGLEC8, MRP4, and CA5A).

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Section: Phenotypic Observationssupporting

confidence: 92%

Gene Expression Profiling of Corpus luteum Reveals Important Insights about Early Pregnancy in Domestic Sheep

Pokharel

Peippo

Weldenegodguad

et al. 2020

Genes

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“…findings are based on fewer animals, the results are in line with earlier studies (Rhind et al, 1980;224 Silva et al, 2016) where typically higher litter size is associated with higher embryo mortality and 225 vice versa. It would be of great interest to determine if productivity follows the same pattern in F2 226 (i.e., F1 x F1) crosses, backcrosses and presumably also in a reciprocal cross.…”

Section: Phenotypic Observations 213supporting

confidence: 89%

Gene expression profiling ofcorpus luteumreveals the importance of immune system during early pregnancy in domestic sheep

Pokharel

Peippo²,

Weldenegodguad

et al. 2019

Preprint

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17The majority of pregnancy loss in ruminants occurs during the preimplantation stage, which is thus 18 the most critical period determining reproductive success. While ovulation rate is the major 19 determinant of litter size in sheep, interactions among the conceptus, corpus luteum and endometrium 20 are essential for pregnancy success. To evaluate the role of reproductive tract function in sheep 21 fertility, we performed a comparative transcriptome study by sequencing total RNA (mRNA and 22 miRNA) from corpus luteum (CL) and endometrium tissues collected during the preimplantation 23 stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287 genes and 599 miRNAs 24were expressed in our dataset. Ten out of the top 25 most highly expressed genes were shared across 25 tissues, indicating the complementary functions of the CL and endometrium. Moreover, highly 26 expressed autosomal genes in the endometrium and CL were associated with biological processes 27 such as progesterone formation (STAR and HSD3B1) in the CL and facilitation of maternal 28 recognition of pregnancy, trophoblast elongation and implantation (LGALS15, CST3, CST6, and 29 EEF1A1) in the endometrium. In the CL, a group of sialic acid-binding immunoglobulin (Ig)-like 30 lectins (Siglecs), solute carriers (SLC13A5, SLC15A2, SLC44A5) and chemokines (CCL5, CXCL13, 31 CXCL9) were upregulated in Finnsheep, while several multidrug resistance-associated proteins 32(MRPs) were upregulated in Texel ewes. We also identified a novel ERV gene located in a reduced 33FecL locus that is associated with sheep prolificacy and is upregulated in prolific Finnsheep. 34Moreover, we report, for the first time in any species, several genes that are active in the CL during 35 early pregnancy (including SIGLEC13, SIGLEC14, SIGLEC6, MRP4, and CA5A). Importantly, 36 functional analysis of differentially expressed genes suggested that Finnsheep have a better immune 37

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“…Nevertheless, it is believed that in the present study the higher ovulation rate per se was the major cause of the embryo loss observed, particularly in the ewes treated with bovine follicular fluid. Previous studies using the prolific Finnish Landrace Dorset Horn sheep treated with PMSG (Rhind, Robinson, Fraser & McHattie, 1980) have shown that with each increase in ovulation rate above 5, there was a reduction in the percentage of ova represented by viable fetuses at the time of slaughter. Similarly, data from embryo transfer studies (Land & Wilmut, 1977) have shown that doubling the number of embryos transferred resulted in a decrease in the number of lambs born.…”

Section: Discussionmentioning

confidence: 92%

Ovulation rate and embryo survival in Damline ewes after treatment with bovine follicular fluid in the luteal phase of the oestrous cycle

Wallace¹,

McNeilly²,

Baird³

1985

Reproduction

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Treatment of Damline ewes with twice daily i.v. injections of bovine follicular fluid during the luteal phase for 10, 6 or 2 days before prostaglandin-induced luteolysis resulted in an increase in ovulation rate. This was associated with a large rebound increase in plasma concentrations of FSH after the last injection of bovine follicular fluid. While conception rate was not affected by bovine follicular fluid treatment, a higher percentage embryonic loss was observed between Days 3 and 34 of pregnancy in the 10-day treatment group only compared to controls. This reflected the increase in ovulation rate above the optimum for embryonic survival in this breed. The present results suggest that the increase in ovulation rate induced by bovine follicular fluid treatment in the luteal phase of the cycle before mating would result in a significant increase in the number of lambs born.

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Ovulation and embryo survival rates and plasma progesterone concentrations of prolific ewes treated with PMSG

Cited by 22 publications

References 15 publications

Gene Expression Profiling of Corpus luteum Reveals Important Insights about Early Pregnancy in Domestic Sheep

Gene Expression Profiling of Corpus luteum Reveals Important Insights about Early Pregnancy in Domestic Sheep

Gene expression profiling ofcorpus luteumreveals the importance of immune system during early pregnancy in domestic sheep

Ovulation rate and embryo survival in Damline ewes after treatment with bovine follicular fluid in the luteal phase of the oestrous cycle

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