Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol

Roover, R. De; Genicot, G.; Leonard, Samuel L.; Bols, P.E.J.; Dessy, F.

doi:10.1016/j.anireprosci.2004.05.022

Cited by 31 publications

(30 citation statements)

References 30 publications

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“…Significant variations in follicular recruitment and in the number of recovered oocytes were reported among breeds and also between individuals within breeds (De Roover et al, 2005). It is not totally clear why B. taurus has fewer follicles during the follicular waves.…”

Section: Resultsmentioning

confidence: 99%

“…The effects of FSH stimulation in taurine donors could be even better, because of the condition of early follicular atresia in these females. However, the results of FSH stimulation in B. taurus are contradictory (Goodhand et al, 1999;Sirard et al, 1999;De Roover et al, 2005). This variation in results may be due to differences in the adopted protocols, as the FSH was not always used in animals in the same state of follicular development.…”

Section: Introductionmentioning

confidence: 93%

See 1 more Smart Citation

Hormonal protocols for in vitro production of Zebu and taurine embryos

Fernandes

Miyauchi²,

Figueiredo

et al. 2014

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick-up and in vitro production of embryos in both cattle subspecies evaluated.Index terms: Bos indicus, Bos taurus, artificial insemination, follicle growth induction, follicular wave synchronization, FSH stimulation. Protocolos hormonais para produção in vitro de embriões de zebuínos e taurinosResumo -O objetivo deste trabalho foi avaliar os efeitos de protocolos hormonais de sincronização, associados ou não com o estímulo ao desenvolvimento folicular, na recuperação de oócitos e na produção in vitro de embriões de Bos indicus e B. taurus, em diferentes estações. As aspirações foliculares guiadas por ultrassom (n=237) foram realizadas sem pré-tratamento (G1, grupo controle) e após sincronização da onda folicular (G2), ou após sincronização da onda folicular e estímulo do desenvolvimento folicular (G3). Bos indicus produziu mais oócitos e embriões que B. taurus (18,7±0,9 vs. 11,9±0,6 oócitos e 4,8±0,3 vs. 2,1±0,2 embriões). Em média, as produções de oócitos e embriões foram maiores em G3 do que em G2, e ambas foram maiores que em G1, o que levou a uma maior conversão de oócitos em embriões nesses tratamentos. A estação quente ou fria não afetou os resultados de B. indicus, enquanto, em B. taurus, tanto a recuperação de oócitos quanto a produção de embriões foram maiores na estação fria. A sincronização da onda folicular melhora a aspiração folicular e a produção in vitro de embriões, em ambas as subespécies de bovinos avaliadas.Termos para indexação: Bos indicus, Bos taurus, inseminação artificial, indução de crescimento folicular, sincronização da onda folicular, estimulação com FSH.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Hormonal protocols for in vitro production of Zebu and taurine embryos

Fernandes

Miyauchi²,

Figueiredo

et al. 2014

Pesq. agropec. bras.

View full text Add to dashboard Cite

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“…(Average result of eCG (equine chorionic gonadotropin) or FSH (follicle stimulating hormone) application on the production of viable oocytes from Nellore crossbred cows from 4 to 9 years old). Rouillier et al (1996);Goodhand et al (1999); Roover et al (2005) comentam que o tratamento com FSH supera os resultados obtidos nos tratamento como eCG, sendo para estes autores o método de escolha para superovulação de bovinos. Na maioria dos estudos comparando os dois procedimentos, o tratamento com FSH resultou em leve aumento de número de embriões utilizáveis.…”

Section: Resultsunclassified

Recuperação de oócitos e produção in vitro de embriões de vacas estimuladas com FSH ou ECG

Ribeiro

Rigolon

Cavalieri³

et al. 2011

Arch. zootec.

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“…The OPU sessions were carried out with the methodology described by De Roover et al (2005). Oocytes were classified under stereomicroscopy, according to Oropeza et al (2004).…”

Section: Methodsmentioning

confidence: 99%

Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers

et al. 2012

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-The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimulation (6 Holstein and 8 Tabapuã), 500 mg of rbST (Boostin ® ) on D0 and 500 IU of eCG (Folligon) on D2; and control (6 Holstein and 8 Tabapuã), in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen ® ) and embryos were evaluated seven days after fertilization. There was an effect from the interaction of treatment and breed, so that hormonal stimulation increased antral follicle count (2-8 mm) in Tabapuã (29.9±2.6 to 41.4±2.6), but not in Holstein heifers (14.4±2.6 to 15.5±2.6). Tabapuã heifers had higher mean antral follicle count than Holsteins (35.6±1.8 vs. 15.0±2.1). The number of viable oocytes was not increased by stimulation in Tabapuã (from 4.7±1.0 to 5.2±1.1 in control and simulation, respectively) or in Holstein heifers (1.3±1.9 to 2.0±1.6 in control and simulation, respectively). There was no difference in the percentage of heifers with more than five viable oocytes in the group treated (33 vs 27%). The number of blastocysts was not affected by treatment (1.75 vs. 1.00 in hormonal stimulation and control, respectively). The increase in antral follicle count in the stimulated Tabapuã heifers did not reflect upon oocyte yield. The differential breed response to the hormonal treatment underscores the need for additional tests, especially for the Holstein breed, in order to enhance OPU efficiency.

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Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol

Cited by 31 publications

References 30 publications

Hormonal protocols for in vitro production of Zebu and taurine embryos

Hormonal protocols for in vitro production of Zebu and taurine embryos

Recuperação de oócitos e produção in vitro de embriões de vacas estimuladas com FSH ou ECG

Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers

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