Ovum Transport Through Rabbit Oviducts Perfused With 6-Hydroxydopamine

Abstract

Summary. Ovum

Effects of Sympathetic Denervation of the Rabbit Oviduct on Normal Ovum Transport and on Transport Modified by Estrogen and Progesterone

“…We could find no data in the literature concerning the effects of surgical denervation on ovum transport. Our findings in animals dcncrvatcd with 6-hydroxydopamine are similar to those of Eddy and Black [10] who found minimal effects of 6-hydroxydopamine on ovum transport. Lack of marked influence of reserpine on ovum transport rates was noted by Bodkhe and Harper [3].…”

“…If these cautions are observed, the technique allows semiquantitative estimates of the catechol amine content of the innervation. The degree of apparent denervation achieved by the various techniques was similar to that reported by others [3,10,17].…”

“…The adrenal medulla, which is unaffected by this agent, increases its secretory rate, apparently to compensate for the peripheral sym pathectomy. The liberation of adrenal catecholamines into the circulation therefore increases after intravenous administration of 6-hydroxydopamine [6], Local perfusion of 6-hydroxydopamine used in these experiments ap pears to minimize systemic effects [10], However, the observation that animals treated with phenoxybenzamine and 6-hydroxydopamine were not significantly different from controls, whereas those treated with 6-hydroxydopamine alone were, suggests that denervation with 6-hydroxydopamine may be associated with some degree of denervation supersensitivity or postsynaptic a-adrenoceptor stimulation.…”

The Autonomic Nervous System and its Relationship to Tubal Ovum Transport – a Reappraisal

“…These studies have also failed to demonstrate total disruption of ovum transport by alteration of norepinephine levels within the oviduct. E ddy and Black [35] observed a decrease in the distance travelled by ova in denervaled oviducts when animals were killed at 60 h after coitus but there were no differences between controls and denervated oviducts at 48 and 72 h after coitus. Similarly, Pauerstein et al [82] found no difference in tubal ovum recovery at 48 and 72 h after HCG injection in animals with norepinephine-depleted oviducts.…”

“…An alternative approach to the investigation of the physiological role of the sympathetic nervous system has utilized depletion of neurotransmit ter by surgical denervation [82], 6-hydroxydopamine [35,82], or reserpine [18,82] or elevation of norepinephine levels by monoamine oxidase in hibition [18]. These studies have also failed to demonstrate total disruption of ovum transport by alteration of norepinephine levels within the oviduct.…”