OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity

Ilves, Tiina; Harvima, Ilkka T.

doi:10.1111/j.1468-3083.2012.04587.x

Cited by 34 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The description of study subjects has been presented in our previous article (24). Briefly, 17 patients with moderate-to-severe AD (11 women and 6 men, mean age 35 years, range 19-69) volunteered to take part in the study after providing written informed consent.…”

Section: Study Subjects and Skin Biopsiesmentioning

confidence: 99%

“…The number and the percentage of IL-6 + MCs were compared to clinical parameters: the Rajka-Langeland Severity Index (mean 7 ± 1), the EASI score (mean 12.3 ± 6.6), the DLQI score (mean 12.7 ± 6.8), the levels of itching (mean 5.4 ± 2), and the total IgE serum levels of study subjects (1,862.3 ± 1,873.6 kU/l) (24). No correlations were detected between these parameters and the number or percentage of IL-6 + MCs.…”

Section: No Correlations Between Clinical Severity Of Ad and Il-6 + Mmentioning

confidence: 99%

See 1 more Smart Citation

Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis

Ilves¹,

Harvima²

2015

Acta Derm Venerol

View full text Add to dashboard Cite

Mast cell chymase and interleukin (IL)-6 can be involved in atopic dermatitis and chymase can degrade IL-6. Our aim was therefore to study the expression of mast cell chymase and IL-6 in atopic skin using enzyme- and immunohistochemistry and to analyse their interaction in vitro. Chymase activity was significantly reduced in mast cells in skin lesions whereas the percentage of IL-6(+) mast cells was increased. Low recombinant human (rh)-chymase concentration (10-100 ng/ml) stimulated and higher concentration (1,000 ng/ml) inhibited the proliferation of T cells and peripheral blood mononuclear cells. Rh-IL-6 inhibited T-cell proliferation, and even inhibited the proliferation induced by rh-chymase. Pretreatment of rh-IL-6 with a high rh-chymase concentration prevented the IL-6-induced inhibition in T-cell proliferation. The results suggest that reduction in chymase activity can give rise to increased cellular effects of mast cell IL-6 and that chymase and IL-6 can modify each other's effects.

show abstract

Section: Study Subjects and Skin Biopsiesmentioning

confidence: 99%

Section: No Correlations Between Clinical Severity Of Ad and Il-6 + Mmentioning

confidence: 99%

Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis

Ilves¹,

Harvima²

2015

Acta Derm Venerol

View full text Add to dashboard Cite

show abstract

“…in the absence of rh-SCF, and were stimulated with a dilution series of rh-IL-33 (R&D Systems) (as described in Results) for 2 days. 3 H-thymidine (85 Ci/mmol, Perkin-Elmer, Boston, MA, USA) in RPMI-1640 medium (Invitrogen) was added to the wells, and the cells were pulsed overnight, as described (20). The LAD2 cells were harvested using Combi Cell Harvester (Skatron Instruments AS, Lier, Norway) and the radioactivity was analysed with a liquid scintillation counter using ULTIMA Gold ™ liquid scintillation fluid (Perkin-Elmer) (20).…”

Section: Stimulation Of Lad2 Mast Cells With Il-33mentioning

confidence: 99%

Experimentallu Induced Psoriatic Lesions Associate with Rapid but Transient Decrease in Interleukin-33 Immunostaining in Epidermis

Suttle¹,

Enoksson²,

Zoltowska³

et al. 2015

Acta Derm Venerol

View full text Add to dashboard Cite

A slight epidermal damage can induce the Köbner reaction in psoriasis, and the “alarmin”, interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and 3 days or at 0 day, 1 day and 7 days for immunohistochemistry. Eight patients out of 18 with the positive Köbner reaction showed a decrease in epidermal thickness and revealed transient reduction in epidermal nuclear immunostaining of IL-33 in 2-h, 1-day, 3-day biopsies compared to the 10 Köbner-negative patients. In keratinocyte cultures, the full-length 32-kDa IL-33 was detected after damaging the cells with freeze-thawing. Interestingly, a very low concentration of rh-IL-33 (0.001–0.01 ng/ml) significantly stimulated (3)H-thymidine uptake by human LAD2 mast cells, but not by psoriatic peripheral blood mononuclear cells. The results show that epidermal IL-33 associates with positive Köbner response, and only a small amount of the IL-33 apparently released may induce proliferation in dermal mast cells.

show abstract

“…27 In summary, 17 subjects with moderate-tosevere AD (11 women and 6 men; mean age, 35 years; range, 19Y69 years) participated in the study after providing written informed consent. Atopic dermatitis severity was measured by the Rajka-Langeland Severity Index (score range, 3Y9; scores 3Y4 denote mild, 4.5Y7.5 denote moderate, and 8Y9 denote severe disease).…”

Section: Study Subjects: Skin Biopsiesmentioning

confidence: 99%

“…Entry criteria are described in our previous article. 27 Four-millimeter punch biopsies were collected from lesional and nonlesional skin under local anesthesia. The nonlesional skin biopsy was taken at least 2 cm away from lesional skin.…”

Section: Study Subjects: Skin Biopsiesmentioning

confidence: 99%

Epidermal Expression of Filaggrin/Profilaggrin Is Decreased in Atopic Dermatitis

et al. 2015

View full text Add to dashboard Cite

show abstract

OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity

Abstract: Our findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction.

Cited by 34 publications

References 41 publications

Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis

Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis

Experimentallu Induced Psoriatic Lesions Associate with Rapid but Transient Decrease in Interleukin-33 Immunostaining in Epidermis

Epidermal Expression of Filaggrin/Profilaggrin Is Decreased in Atopic Dermatitis

Contact Info

Product

Resources

About