OX40L/OX40 Signal Promotes IL-9 Production by Mucosal MAIT Cells During Helicobacter pylori Infection

Ming, Siqi; Zhang, Mei; Liang, Zhongxing; Li, Chunna; He, Jianzhong; Chen, Peiyu; Zhang, Shunxian; Niu, Xiaoli; Deng, Shimei; Geng, Lanlan; Zhang, Guoliang; Gong, Sitang; Wu, Yongjian

doi:10.3389/fimmu.2021.626017

Cited by 12 publications

(10 citation statements)

References 55 publications

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“…In addition, no differences in gastric Vα7.2 + CD161 hi T cell frequencies between H. pylori-infected and H. pylori-uninfected groups were observed despite being the local area of infection; and this may be due to our limitation of our sample population in that all gastric samples had gastritis. 6,7 Nonetheless, Ming et al 65 have shown that MAIT cells produce high levels of IL-9 and these IL-9-producing MAIT cells are related to H. pylori-infected individuals, of which all had gastritis, further suggesting MAIT cell relation to H. pylori infection.…”

Section: Discussionmentioning

confidence: 99%

Human mucosal Vα7.2+CD161hi T cell distribution at physiologic state and inHelicobacter pyloriinfection

Boonpattanaporn

Kongkaew

Sengprasert

et al. 2022

Journal of Leukocyte Biology

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Mucosal‐associated invariant T (MAIT) cells are innate‐like, unconventional T cells that are present in peripheral blood and mucosal surfaces. A clear understanding of how MAIT cells in the mucosae function and their role in host immunity is still lacking. Therefore, our aim was to investigate MAIT cell distribution and their characteristics in the gastrointestinal (GI) mucosal tissue based on Vα7.2+CD161hi identification. We showed that Vα7.2+CD161hi T cells are present in both intraepithelial layer and lamina propriae of the GI mucosa, but have different abundance at each GI site. Vα7.2+CD161hi T cells were most abundant in the duodenum, but had the lowest reactivity to MR1‐5‐OP‐RU tetramers when compared with Vα7.2+CD161hi T cells at other GI tissue sites. Striking discrepancies between MR1‐5‐OP‐RU tetramer reactive cells and Vα7.2+CD161hi T cells were observed along each GI tissue sites. Vα7.2+CD161hi TCR repertoire was most diverse in the ileum. Similar dominant profiles of TRBV usage were observed among peripheral blood, duodenum, ileum, and colon. Some TRBV chains were detected at certain intestinal sites and not elsewhere. The frequency of peripheral blood Vα7.2+CD161hi T cells correlated with mucosal Vα7.2+CD161hi T cells in lamina propriae ileum and lamina propriae colon. The frequency of peripheral blood Vα7.2+CD161hi T cells in Helicobacter pylori‐infected individuals was significantly lower than uninfected individuals, but this was not observed with gastric Vα7.2+CD161hi T cells. This study illustrates the biology of Vα7.2+CD161hi T cells in the GI mucosa and provides a basis for understanding MAIT cells in the mucosa and MAIT‐related GI diseases.

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Section: Discussionmentioning

confidence: 99%

Human mucosal Vα7.2+CD161hi T cell distribution at physiologic state and inHelicobacter pyloriinfection

Boonpattanaporn

Kongkaew

Sengprasert

et al. 2022

Journal of Leukocyte Biology

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“…Mucosal mononuclear cells were isolated from the gastric mucosa biopsy of the patients as previously described ( 4 ). Briefly, biopsy specimens were digested with shaking at 37°C for 45 min.…”

Section: Methodsmentioning

confidence: 99%

“…Paraffin-embedded samples were cut into 5-μm slices, and immunohistochemistry was conducted as previously described ( 4 ). In brief, the mucosa was fixed with paraformaldehyde, followed by antigen retrieval with the EDTA antigen retrieval solution (Servicebio).…”

Section: Methodsmentioning

confidence: 99%

“…Gastric mucosa was collected and homogenized by bead-milling as previously described ( 4 ). The cells were lysed, and total RNA was extracted with TRIzol reagent (Invitrogen) (refer to the instructions of the manufacturer).…”

Section: Methodsmentioning

confidence: 99%

“…Meanwhile, the inflammatory state in the mucosa could, in turn, impact the biological behavior of H. pylori ( 3 ). We previously demonstrated that Th9-like MAIT cells are enriched in gastric tissue and revealed the positive correlation of Th9-producing MAIT cells with disease progression ( 4 ). We also reported that H. pylori -associated antigens induced the Th1 and Th17 response of gastric CD4 + T cells ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

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GITR Promotes the Polarization of TFH-Like Cells in Helicobacter pylori-Positive Gastritis

Ming¹,

Yin²,

Li³

et al. 2021

Front. Immunol.

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Gastric CD4+T cells contribute to Helicobacter pylori (H. pylori)-induced gastritis by amplifying mucosal inflammation and exacerbating mucosal injuries. However, the pathogenic CD4+ T cell subset involved in gastritis and the potential regulators are still unclear. Here we identified an IL-21-producing gastric CD4+T cell subset, which exhibited tissue-resident CXCR5−BTLA−PD-1hi TFH-like phenotype in H. pylori-positive gastritis patients. Meanwhile, we identified glucocorticoid-induced tumor necrosis factor receptor (GITR) as an important regulator to facilitate IL-21 production by CD4+T cells and accelerate mucosal inflammation in gastritis patients with H. pylori infection. Moreover, GITR expression was increased in gastric CD4+T cells of gastritis patients compared to healthy controls, along with the upregulated expression of its ligand GITRL in mucosal macrophages (Mϕ) of gastritis patients. Further observations showed that the activation of GITR/GITRL signal promoted the IL-21 production of CD4+T cells via the STAT3 pathway. Besides this, IL-21 from CD4+T cells induced the proliferation of B cell and promoted the production of inflammatory cytokines IL-1β and IL-6 and chemokines MIP-3α and CCL-25 as well as matrix metalloproteinase (MMP)-3 and MMP-9 by human gastric epithelial cells, suggesting the facilitating effect of IL-21-producing CD4+T cells on mucosal inflammation and injuries. Taking these data together, we revealed that GITR/GITRL signal promoted the polarization of mucosal IL-21-producing CD4+T cells in H. pylori-positive gastritis, which may provide therapeutic strategies for the clinical treatment of H. pylori-induced gastritis.

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Mucosal-Associated Invariant T Cells in the Digestive System: Defender or Destroyer?

Zhang

Shen

Zhou

et al. 2023

Cellular and Molecular Gastroenterology and Hepatology

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OX40L/OX40 Signal Promotes IL-9 Production by Mucosal MAIT Cells During Helicobacter pylori Infection

Cited by 12 publications

References 55 publications

Human mucosal Vα7.2+CD161hi T cell distribution at physiologic state and inHelicobacter pyloriinfection

Human mucosal Vα7.2+CD161hi T cell distribution at physiologic state and inHelicobacter pyloriinfection

GITR Promotes the Polarization of TFH-Like Cells in Helicobacter pylori-Positive Gastritis

Mucosal-Associated Invariant T Cells in the Digestive System: Defender or Destroyer?

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