Oxacillinase-181 Carbapenemase-Producing <i>Klebsiella pneumoniae</i> in Neonatal Intensive Care Unit, Ghana, 2017–2019

Nielsen, Karen Leth; Marvig, Rasmus Lykke; Bjerrum, Stephanie; Enweronu‐Laryea, Christabel; Bennedbæk, Marc; Newman, Mercy J.; Ayibor, Prosper K; Andersen, Leif Percival; Kurtzhals, Jørgen A. L.

doi:10.3201/eid2609.200562

Cited by 21 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OXA-181-encoding gene was first reported in 2007 and was subsequently identified in several countries, including its recent detection in Ghana [ 15 , 16 , 33 ]. It is mainly found in E. coli and K. pneumoniae strains with an increasing prevalence [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…A recent study by Labi and colleagues revealed a significant proportion of OXA-181-producing Klebsiella spp . among a neonatal population in a tertiary hospital in Ghana [ 15 , 16 ]. This finding suggests that the prevalence of bla O XA-181 is underreported in the general population, as most clinical laboratories are not equipped for its detection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

Prah

Ayibieke

Mahazu

et al. 2021

Emerging Microbes & Infections

View full text Add to dashboard Cite

The emergence and spread of carbapenemase-producing bacteria are serious threats to public health. We characterized two OXA-181-producing Escherichia coli isolates from pediatric patients with diarrhea from Ghana. bla OXA - 181 was localized on the self-conjugative IncX3-containing plasmid in the E. coli ST410 isolate, belonging to an emerging lineage, and an IncFIC(FII)-containing plasmid in E. coli ST940. The bla OXA-181 - qnrS1 region was found on the IS 26 composite transposon, which contained a 366-bp deletion in the region encoding the Rep A protein for the IncX3-containing plasmid. The IncFIC(FII) plasmid was novel and integrated with an approximately 39-kb IncX1 plasmid through conjugal transfer. Both plasmids clustered close to plasmids from Switzerland. To the best of our knowledge, this is the first report describing the presence of an IncX3 plasmid containing bla OXA-181 in strains closely related to the B4/H24RxC clade in Africa, suggesting its emergence and the need to strengthen antimicrobial resistance surveillance.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

Prah

Ayibieke

Mahazu

et al. 2021

Emerging Microbes & Infections

View full text Add to dashboard Cite

show abstract

“…All the used reference genomes had average nucleotide identity ≥95% to our isolates as identified by fastANI (22). BacDist is an in-house developed workflow which was also used in other microbial genomics studies (23,24). BacDist is a Snakemake workflow (25) which first performs variant calling with Snippy 4 (26) using minimum read mapping quality of 50, minimum read coverage of 10, and minimum fraction of reads supporting the variant of 0.5 for each sample from the submitted sample group.…”

Section: Patient-to-patient Transmission Identificationmentioning

confidence: 99%

Transmission and Antibiotic Resistance of Achromobacter in Cystic Fibrosis

et al. 2021

Self Cite

View full text Add to dashboard Cite

Achromobacter species are increasingly being detected in patients with cystic fibrosis (CF), and this emerging pathogen is associated with antibiotic resistance and more severe disease outcomes. Nonetheless, little is known about the extent of transmission and antibiotic resistance development in Achromobacter infections. We sequenced the genomes of 101 clinical isolates of Achromobacter (A. xylosoxidans based on MALDI-TOF/API N20 typing) collected from 51 patients with CF—the largest longitudinal dataset to-date. We performed phylogenetic analysis on the genomes and combined this with epidemiological and antibiotic resistance data to identify patient-to-patient transmission and development of antibiotic resistance. We confirmed that MALDI-TOF/API N20 was not sufficient for Achromobacter species-level typing, and that the population of Achromobacter isolates was composed of five different species where A. xylosoxidans accounted for 52% of infections. Most patients were infected by unique Achromobacter clone types; nonetheless, suspected patient-to-patient transmission cases identified by shared clone types were observed in 35% (N=18) of patients. In 15 of 16 cases the suspected transmissions were further supported by genome- or clinic visit-based epidemiological analysis. Finally, we found that resistance developed over time. We show that whole-genome sequencing (WGS) is essential for Achromobacter species typing and patient-to-patient transmission identification which was identified in A. ruhlandii, A. xylosoxidans and, for the first time, A. insuavis. Furthermore, we show that the development of antibiotic resistance is associated with chronic Achromobacter infections. Our findings emphasize that transmission and antibiotic resistance should be considered in future treatment strategies.

show abstract

“…Treatment of infections caused by carbapenem-resistant pathogens is quite a big challenge in industrialized countries and almost impossible for health systems with limited resources [ 9 , 10 , 11 ]. Of great concern are carbapenemase-producing A. baumannii , P. aeruginosa and Enterobacterales [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

High Prevalence of Carbapenemase-Producing Acinetobacter baumannii in Wound Infections, Ghana, 2017/2018

et al. 2021

View full text Add to dashboard Cite

Three years after a prospective study on wound infections in a rural hospital in Ghana revealed no emergence of carbapenem-resistant bacteria we initiated a new study to assess the prevalence of multidrug-resistant pathogens. Three hundred and one samples of patients with wound infections were analysed for the presence of resistant bacteria in the period August 2017 till March 2018. Carbapenem-resistant Acinetobacter (A.) baumannii were further characterized by resistance gene sequencing, PCR-based bacterial strain typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST “Oxford scheme”). A. baumanni was detected in wound infections of 45 patients (15%); 22 isolates were carbapenem-resistant. Carbapenemases NDM-1 and/or OXA-23 were detected in all isolates; two isolates harboured additionally OXA-420. PFGE and MLST analyses confirmed the presence of one A. baumannii strain in 17 patients that was assigned to the worldwide spread sequence type ST231 and carried NDM-1 and OXA-23. Furthermore, two new A. baumannii STs (ST2145 and ST2146) were detected in two and three patients, respectively. Within three years the prevalence of carbapenem-resistant A. baumannii increased dramatically in the hospital. The early detection of multidrug-resistant bacteria and prevention of their further spread are only possible if continuous surveillance and molecular typing will be implemented.

show abstract

Oxacillinase-181 Carbapenemase-Producing Klebsiella pneumoniae in Neonatal Intensive Care Unit, Ghana, 2017–2019

Cited by 21 publications

References 11 publications

Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

Transmission and Antibiotic Resistance of Achromobacter in Cystic Fibrosis

High Prevalence of Carbapenemase-Producing Acinetobacter baumannii in Wound Infections, Ghana, 2017/2018

Contact Info

Product

Resources

About