Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis

Grąz, Marcin; Rachwał, Kamila; Zan, R.; Jarosz-Wilkołazka, Anna

doi:10.18388/abp.2016_1282

Cited by 14 publications

(8 citation statements)

References 38 publications

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“…Protein homologs that are 100% identical were then removed, leaving 2519 OXDD, 1556 FRC, and 1037 OXC unique homologs, which were used as a reference database of ODEs for subsequent query against the meta’omics data. Oxalate oxidoreductase ( Figure 1A ), a recently discovered enzyme for which there only is limited information ( Anand et al, 2002 ; Dumas et al, 1993 ; Grąz et al, 2016 ; Kumar et al, 2011 ; Svedruzić et al, 2007 ; Tanner et al, 2001 ), was not considered in this present study.…”

Section: Methodsmentioning

confidence: 99%

Microbial genetic and transcriptional contributions to oxalate degradation by the gut microbiota in health and disease

Liu

Devlin

et al. 2021

eLife

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Over-accumulation of oxalate in humans may lead to nephrolithiasis and nephrocalcinosis. Humans lack endogenous oxalate degradation pathways (ODP), but intestinal microbes can degrade oxalate using multiple ODPs and protect against its absorption. The exact oxalate-degrading taxa in the human microbiota and their ODP have not been described. We leverage multi-omics data (>3000 samples from >1000 subjects) to show that the human microbiota primarily uses the type II ODP, rather than type I. Further, among the diverse ODP-encoding microbes, an oxalate autotroph, Oxalobacter formigenes, dominates this function transcriptionally. Patients with Inflammatory Bowel Disease (IBD) frequently suffer from disrupted oxalate homeostasis and calcium oxalate nephrolithiasis. We show that the enteric oxalate level is elevated in IBD patients, with highest levels in Crohn's disease patients with both ileal and colonic involvement consistent with known nephrolithiasis risk. We show that the microbiota ODP expression is reduced in IBD patients, which may contribute to the disrupted oxalate homeostasis. The specific changes in ODP expression by several important taxa suggest that they play distinct roles in IBD-induced nephrolithiasis risk. Lastly, we colonize mice that are maintained in the gnotobiotic facility with O. formigenes, using either a laboratory isolate or an isolate we cultured from human stools, and observed a significant reduction in host fecal and urine oxalate levels, supporting our in silico prediction of the importance of the microbiome, particularly O. formigenes in host oxalate homeostasis.

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Section: Methodsmentioning

confidence: 99%

Microbial genetic and transcriptional contributions to oxalate degradation by the gut microbiota in health and disease

Liu

Devlin

et al. 2021

eLife

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“…Among them, glucose oxidase (EC 1.1.3.4), glyoxal oxidase (GLOX; EC 1.2.3.5), aryl alcohol oxidases (AAO; EC 1.1.3.7), pyranose 2-oxidase (POX; EC 1.1.3.10), and cellobiose dehydrogenase (CDH; EC 1.1.99.18) are very important and well establish (Janusz et al 2017 ). We postulated for OXO from A. biennis such a role in fungal metabolism in our earlier study (Graz et al 2016 ). Oxalic acid supplementation can stimulate the secretion of MnP in C. subvermispora (Aguiar and Ferraz 2012 ).…”

Section: Discussionmentioning

confidence: 87%

“…It has been detected in barley (Kotsira and Clonis 1997 ; Requena and Bornemann 1999 ), wheat (Hu and Guo 2009 ), oats, rice, and rye (Lane 2000 ), maize (Vuletic and Sukalovic 2000 ; Lane 2000 ), and beet leaves and stems (Varalakshmi and Richardson 1992 ). We were prompted to look for oxalate oxidase in fungi by the fact that the ability to degrade oxalate via the oxidative pathway has been proved so far only in C. subvermispora (Aguilar et al 1999 ; Escutia, et al 2005 ) and A. biennis (Grąz et al 2009 , 2016 ). It is worth noticing that the well-characterized OXO from C. subvermispora is classified as a bicupin protein similar to fungal ODC, and the known OXO originating from plants are monocupins (Dunwell et al 2000 ; Escutia et al 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Oxalic acid degradation in wood-rotting fungi. Searching for a new source of oxalate oxidase

Grąz

Ruminowicz-Stefaniuk

Jarosz-Wilkołazka

2022

World J Microbiol Biotechnol

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Oxalate oxidase (EC 1.2.3.4) is an oxalate-decomposing enzyme predominantly found in plants but also described in basidiomycete fungi. In this study, we investigated 23 fungi to determine their capability of oxalic acid degradation. After analyzing their secretomes for the products of the oxalic acid-degrading enzyme activity, three groups were distinguished among the fungi studied. The first group comprised nine fungi classified as oxalate oxidase producers, as their secretome pattern revealed an increase in the hydrogen peroxide concentration, no formic acid, and a reduction in the oxalic acid content. The second group of fungi comprised eight fungi described as oxalate decarboxylase producers characterized by an increase in the formic acid level associated with a decrease in the oxalate content in their secretomes. In the secretomes of the third group of six fungi, no increase in formic acid or hydrogen peroxide contents was observed but a decline in the oxalate level was found. The intracellular activity of OXO in the mycelia of Schizophyllum commune, Trametes hirsuta, Gloeophyllum trabeum, Abortiporus biennis, Cerrena unicolor, Ceriosporopsis mediosetigera, Trametes sanguinea, Ceriporiopsis subvermispora, and Laetiporus sulphureus was confirmed by a spectrophotometric assay.

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“…Oxalate oxidoreductase (Fig. 1A), a recently discovered enzyme with limited information [51,52,[97][98][99][100][101], was not considered in this present study.…”

Section: Methodsmentioning

confidence: 99%

Microbial contributions to oxalate metabolism in health and disease

Liu

Devlin

et al. 2020

Preprint

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Over-accumulation of oxalate in humans may lead to nephrolithiasis and nephrocalcinosis. Humans lack endogenous oxalate degradation pathways (ODP), but intestinal microbiota can degrade oxalate and protect against its absorption. However, the particular microbes that actively degrade oxalate in vivo are ill-defined, which restricts our ability to disentangle the underlying taxonomic contributions. Here we leverage large-scale multi-omics data (>3000 samples from >1000 subjects) to show that the human microbiota in health harbors diverse ODP-encoding microbial species, but an oxalate autotroph-Oxalobacter formigenes- dominates this function transcriptionally. Patients with Inflammatory Bowel Disease (IBD) are at significantly increased risk for disrupted oxalate homeostasis and calcium-oxalate nephrolithiasis. Here, by analyzing multi-omics data from the iHMP-IBD study, we demonstrate that the oxalate degradation function conferred by the intestinal microbiota is severely impaired in IBD patients. In parallel, the enteric oxalate levels of IBD patients are significantly elevated and associated with intestinal disease severity, which is consistent with the clinically known nephrolithiasis risk. The specific changes in ODP expression by several important taxa suggest that they play different roles in the IBD-induced nephrolithiasis risk.

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Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis

Cited by 14 publications

References 38 publications

Microbial genetic and transcriptional contributions to oxalate degradation by the gut microbiota in health and disease

Microbial genetic and transcriptional contributions to oxalate degradation by the gut microbiota in health and disease

Oxalic acid degradation in wood-rotting fungi. Searching for a new source of oxalate oxidase

Microbial contributions to oxalate metabolism in health and disease

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