OXI1 and DAD Regulate Light-Induced Cell Death Antagonistically through Jasmonate and Salicylate Levels

Beaugelin, Inès; Chevalier, Anne; D’Alessandro, Stefano; Ksas, Brigitte; Novák, Ondřej; Strnad, Miroslav; Forzani, Céline; Hirt, Heribert; Havaux, Michel; Monnet, Fabien

doi:10.1104/pp.19.00353

Cited by 34 publications

(38 citation statements)

References 77 publications

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“…Plant BAG proteins function in cytoprotection under stress conditions ( Kabbage and Dickman, 2008 ). DAD regulates light-induced cell death antagonistically through jasmonate and salicylate levels ( Beaugelin et al., 2019 ). The expression of MhBAG and MhBI-1 was associated with root cell death in Malus hupehensis ( Fan et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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Excessive cadmium (Cd) damages plants by causing cell death. The present study discusses the function of natural resistance-associated macrophage protein (NRAMP) on cell death caused by Cd in Malus hupehensis. MhNRAMP1 was isolated from M. hupehensis roots, and its protein was located in the cell membrane as a transmembrane protein characterized by hydrophobicity. MhNRAMP1 expression in the roots was induced by Cd stress and calcium (Ca) deficiency. MhNRAMP1 overexpression increased Cd concentration in yeasts and enhanced their sensitivity to Cd. Phenotypic comparisons of plants under Cd stress revealed that the growth of transgenic tobacco and apple calli overexpressing MhNRAMP1 was worse than that of the wild type (WT). The Cd 2+ influx of transgenic tobacco roots and apple calli was higher, and the recovery time of the Cd 2+ influx to a stable state in transgenic apple calli was longer than that of the WT. Cd accumulation and the percentage of apoptotic cells in transgenic lines were higher. Correspondingly, the caspase-1-like and vacuolar processing enzyme (VPE) activities and MdVPEg expression were higher in transgenic apple calli, but the expression levels of genes that inhibit cell death were lower than those in the WT under Cd stress. Moreover, the Cd translocation from the roots to leaves was increased after MhNRAMP1 overexpression, but the Cd translocation from the leaves to seeds was not affected. These results suggest that MhNRMAP1 exacerbated Cd-induced cell death, which was accomplished by mediating Cd 2+ uptake and accumulation, as well as stimulating VPE.

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Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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“…However, in contrast to flu, in ch1 EX1 does not participate in the onset of PCD [80]. In ch1, 1 O 2 -dependent PCD involves a serine-threonine kinase OXIDATIVE SIGNAL INDUCIBLE1 (OXI1), which was previously shown to regulate H 2 O 2 -mediated processes including those related to the response to pathogens [94,95]. In a ch1/oxi1 double mutant, high light leads to 1 O 2 generation but not to PCD [94].…”

Section: Roles Of 1 O 2 In Plant Stress Responsementioning

confidence: 98%

“…In a ch1/oxi1 double mutant, high light leads to 1 O 2 generation but not to PCD [94]. OXI1 signaling involves phytohormones: expression of genes encoding enzymes involved in JA and SA biosynthesis is strongly increased in plants overexpressing OXI1 (OE-OXI1) even in the absence of light stress, while in an oxi1 knockout mutant the levels of SA, but not JA, were decreased [95]. Only a small number of genes is activated both during OXI-mediated and during EX1-mediated cell death [96].…”

Section: Roles Of 1 O 2 In Plant Stress Responsementioning

confidence: 99%

Section: Detection Of 1 O 2 In Plantsmentioning

confidence: 99%

“…The equipment for detection of autoluminescence includes a high-sensitivity cooled CCD camera, a 50-mm focal lens and a set of color filters for the analysis of the spectral characteristics of the autoluminescence [139]. Autoluminescence imaging allows non-invasive quantitation of early symptoms of oxidative stress in plants which can be detected much earlier than visual signs such as chlorophyll bleaching or turgor loss (e.g., [75,76,94,95]). Detached leaves of Arabidopsis wild-type Col-0 (b) or the ch1-3 mutant (c) were infiltrated with SOSG (5 µM) and exposed to light (PPFD 233 µmol m −2 s −1 ) for 2 h. The leaves were then photographed using an Olympus BX51 microscope (Olympus Optical Co., Germany) equipped with a ColorView II digital camera and CellˆF software (Olympus, Hamburg, Germany), with an exposure time of 500 ms, using blue excitation filter (BP 460-490, DM 505, BA 510-550).…”

Section: Detection Of 1 O 2 In Plantsmentioning

confidence: 99%

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Singlet Oxygen in Plants: Generation, Detection, and Signaling Roles

Dmitrieva

Tyutereva

Voitsekhovskaja

2020

IJMS

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Singlet oxygen (1O2) refers to the lowest excited electronic state of molecular oxygen. It easily oxidizes biological molecules and, therefore, is cytotoxic. In plant cells, 1O2 is formed mostly in the light in thylakoid membranes by reaction centers of photosystem II. In high concentrations, 1O2 destroys membranes, proteins and DNA, inhibits protein synthesis in chloroplasts leading to photoinhibition of photosynthesis, and can result in cell death. However, 1O2 also acts as a signal relaying information from chloroplasts to the nucleus, regulating expression of nuclear genes. In spite of its extremely short lifetime, 1O2 can diffuse from the chloroplasts into the cytoplasm and the apoplast. As shown by recent studies, 1O2-activated signaling pathways depend not only on the levels but also on the sites of 1O2 production in chloroplasts, and can activate two types of responses, either acclimation to high light or programmed cell death. 1O2 can be produced in high amounts also in root cells during drought stress. This review summarizes recent advances in research on mechanisms and sites of 1O2 generation in plants, on 1O2-activated pathways of retrograde- and cellular signaling, and on the methods to study 1O2 production in plants.

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Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

Ksas

Havaux

2022

Methods in Molecular Biology

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OXI1 and DAD Regulate Light-Induced Cell Death Antagonistically through Jasmonate and Salicylate Levels

Cited by 34 publications

References 77 publications

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

Singlet Oxygen in Plants: Generation, Detection, and Signaling Roles

Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

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