Oxidation and reduction of cytochrome c by mitochondrial enzymes of<i>Setaria cervi</i>

Goyal, Neena; Srivastava, V. M. L.

doi:10.1017/s0022149x00013778

Cited by 23 publications

(20 citation statements)

References 15 publications

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“…(), glutamine synthetase according to Temple et al . (), cellulose synthase according to Wood & Bhat (), phosphoglycolate phosphatase according to Ames () and cytochrome oxidase according to Goyal & Srivastava (). NADH dehydrogenase activity was measured according to Galante & Hatefi () with a modified reaction mixture (1 mL) containing 50 m m phosphate buffer (pH 7.4), 0.1% Triton X‐100 (v/v), 1.6 m m potassium ferricyanide, 0.17 m m NADH and 30 μg mitochondrial protein in phosphate buffer.…”

Section: Methodsmentioning

confidence: 99%

“…Enzyme activities were determined using existing protocols. Ascorbate peroxidase and monodehydroascorbate reductase were measured according to Murshed et al (2008), peroxiredoxin according to Horling et al (2003), superoxide dismutase according to Dhindsa et al (1981), glutathione S-transferase according to Habig et al (1974), o-aminophenol oxidase according to Suzuki et al (2006), glutamine synthetase according to Temple et al (1996), cellulose synthase according to Wood & Bhat (1988), phosphoglycolate phosphatase according to Ames (1966) and cytochrome oxidase according to Goyal & Srivastava (1995). NADH dehydrogenase activity was measured according to Galante & Hatefi (1978) with a modified reaction mixture (1 mL) containing 50 mM phosphate buffer (pH 7.4), 0.1% Triton X-100 (v/ v), 1.6 mM potassium ferricyanide, 0.17 mM NADH and 30 lg mitochondrial protein in phosphate buffer.…”

Section: Enzymatic Activitiesmentioning

confidence: 99%

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Molecular response of Sargassum vulgare to acidification at volcanic CO₂ vents: insights from de novo transcriptomic analysis

et al. 2017

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Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carried out on Sargassum vulgare populations growing under acidified conditions at volcanic CO vents and compared with populations in a control site. Several transcripts involved in a wide range of cellular and metabolic processes were differentially expressed. No drastic changes were observed in the carbon acquisition processes and RuBisCO level. Moreover, relatively few stress genes, including those for antioxidant enzymes and heat-shock proteins, were affected. Instead, increased expression of transcripts involved in energy metabolism, photosynthetic processes and ion homeostasis suggested that algae increased energy production to maintain ion homeostasis and other cellular processes. Also, an increased allocation of carbon to cell wall and carbon storage was observed. A number of genes encoding proteins involved in cellular signalling, information storage and processing and transposition were differentially expressed between the two conditions. The transcriptional changes of key enzymes were largely confirmed by enzymatic activity measurements. Altogether, the changes induced by acidification indicate an adaptation of growth and development of S. vulgare at the volcanic CO vents, suggesting that this fleshy alga exhibits a high plasticity to low pH and can adopt molecular strategies to grow also in future more acidified waters.

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Section: Methodsmentioning

confidence: 99%

Section: Enzymatic Activitiesmentioning

confidence: 99%

Molecular response of Sargassum vulgare to acidification at volcanic CO₂ vents: insights from de novo transcriptomic analysis

et al. 2017

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“…The absorbance was measured at 410 nm and NADH-DH activity was calculated using an extinction coefficient of 1 mM -1 cm -1 . COX activity was determined according to Goyal and Srivastava (1995).…”

Section: Methodsmentioning

confidence: 99%

Physiological and Biochemical Analyses Shed Light on the Response of Sargassum vulgare to Ocean Acidification at Different Time Scales

et al. 2017

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Studies regarding macroalgal responses to ocean acidification (OA) are mostly limited to short-term experiments in controlled conditions, which hamper the possibility to scale up the observations to long-term effects in the natural environment. To gain a broader perspective, we utilized volcanic CO2 vents as a “natural laboratory” to study OA effects on Sargassum vulgare at different time scales. We measured photosynthetic rates, oxidative stress levels, antioxidant contents, antioxidant enzyme activities, and activities of oxidative metabolic enzymes in S. vulgare growing at a natural acidified site (pH 6.7) compared to samples from a site with current pH (pH 8.2), used as a control one. These variables were also tested in plants transplanted from the control to the acidified site and vice-versa. After short-term exposure, photosynthetic rates and energy metabolism were increased in S. vulgare together with oxidative damage. However, in natural populations under long-term conditions photosynthetic rates were similar, the activity of oxidative metabolic enzymes was maintained, and no sign of oxidative damages was observed. The differences in the response of the macroalga indicate that the natural population at the acidified site is adapted to live at the lowered pH. The results suggest that this macroalga can adopt biochemical and physiological strategies to grow in future acidified oceans.

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“…NADH‐cytochrome c oxidoreductase activity was measured by following the reduction in oxidized cytochrome c at 565 nm according to the method of Goyal and Srivastava . A total of 1 mL assay mixture contained in addition to enzyme 50 m m phosphate buffer, pH 7.4, 0.1 mg BSA, 20 m m oxidized cytochrome c , and 0.5 μ m NADH.…”

Section: Methodsmentioning

confidence: 99%

Mechanisms of isoproterenol‐induced cardiac mitochondrial damage: protective actions of melatonin

Mukherjee

Ghosh

Dutta

et al. 2015

Journal of Pineal Research

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Mitochondrial dysfunction due to oxidative damage is the key feature of several diseases. We have earlier reported mitochondrial damage resulting from the generation of oxidative stress as a major pathophysiological effect of isoproterenol (ISO)-induced myocardial ischemia in rats. That melatonin is an antioxidant that ameliorates oxidative stress in experimental animals as well as in humans is well established. We previously demonstrated that melatonin provides cardioprotection against ISO-induced myocardial injury as a result of its antioxidant properties. The mechanism of ISO-induced cardiac mitochondrial damage and protection by melatonin, however, remains to be elucidated in vitro. In this study, we provide evidence that ISO causes dysfunction of isolated goat heart mitochondria. Incubation of cardiac mitochondria with increasing concentrations of ISO decreased mitochondrial succinate dehydrogenase (SDH) activity, which plays a pivotal role in mitochondrial bioenergetics, as well as altered the activities of other key enzymes of the Kreb's cycle and the respiratory chain. Co-incubation of ISO-challenged mitochondria with melatonin prevented the alterations in enzyme activity. That these changes in mitochondrial energy metabolism were due to the perpetration of oxidative stress by ISO was evident from the increased levels of lipid peroxidation and decreased reduced glutathione/oxidized glutathione ratio. ISO-induced oxidative stress also altered mitochondrial redox potential and brought about changes in the activity of the antioxidant enzymes manganese superoxide dismutase and glutathione peroxidase, eventually leading to alterations in total ATPase activity and membrane potential. Melatonin ameliorated these changes likely through its antioxidant abilities suggesting a possible mechanism of cardioprotection by this indole against ISO-induced myocardial injury.

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Oxidation and reduction of cytochrome c by mitochondrial enzymes ofSetaria cervi

Abstract: A mitochondria-rich fraction isolated from the cuticle-hypodermis-muscle system of Setaria cervi, a bovine filarial parasite, possessed substrate-

Cited by 23 publications

References 15 publications

Molecular response of Sargassum vulgare to acidification at volcanic CO₂ vents: insights from de novo transcriptomic analysis

Molecular response of Sargassum vulgare to acidification at volcanic CO₂ vents: insights from de novo transcriptomic analysis

Physiological and Biochemical Analyses Shed Light on the Response of Sargassum vulgare to Ocean Acidification at Different Time Scales

Mechanisms of isoproterenol‐induced cardiac mitochondrial damage: protective actions of melatonin

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Oxidation and reduction of cytochrome c by mitochondrial enzymes ofSetaria cervi

Abstract: A mitochondria-rich fraction isolated from the cuticle-hypodermis-muscle system of Setaria cervi, a bovine filarial parasite, possessed substrate-

Cited by 23 publications

References 15 publications

Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: insights from de novo transcriptomic analysis

Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: insights from de novo transcriptomic analysis

Physiological and Biochemical Analyses Shed Light on the Response of Sargassum vulgare to Ocean Acidification at Different Time Scales

Mechanisms of isoproterenol‐induced cardiac mitochondrial damage: protective actions of melatonin

Contact Info

Product

Resources

About

Molecular response of Sargassum vulgare to acidification at volcanic CO₂ vents: insights from de novo transcriptomic analysis

Molecular response of Sargassum vulgare to acidification at volcanic CO₂ vents: insights from de novo transcriptomic analysis