Oxidation in the NADP system and release of GSSG from hemoglobin‐free perfused rat liver during peroxidatic oxidation of glutathione by hydroperoxides

Sies, Helmut; Gerstenecker, C.; Menzel, H.; Flohé, Leopold

doi:10.1016/0014-5793(72)80434-4

Cited by 248 publications

(77 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BHP, in addition, is lipid soluble. Sies et al (17) re ported that BHP is readily extracted by the isolated liver up to certain amounts: e.g., only 3% of the BHP appears in the effluent per fusate under infusion of 0.6 mM BHP and the maximum extraction rate by the liver is calcu lated to be 1.5 umol/g liver/min, although the intralobular distribution of BHP still remains to be determined. Therefore, in the perfusion system, hepatocytes in the inlet zones are ex pected to be exposed to higher concentration of BHP as well as higher oxygen tension and eventually to be more peroxidized than the outlet hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Sies et al (17) and Sies and Summer (25) reported that an infusion of 0.3-0.6 mM BHP for 5 min into hemoglobin-free perfused livers leads to only reversible changes, accompanied by cellular oxidation of GSH through GSH per oxidase and the resultant release of GSSG into the perfusate. In the present study, we in fused 0.8 mM BHP for much longer periods.…”

Section: Discussionmentioning

confidence: 99%

“…Normal rat livers were infused with a final concentration of 0.8 mM of BHP for 15 to 60 min, in which the concentration of BHP and duration of infusion exceeded the hydro peroxide removing capacity of the liver as re ported in a similar liver perfusion system (17). As shown in Fig.…”

Section: Infusion Of Bhpmentioning

confidence: 99%

See 2 more Smart Citations

Histological Detection of Lipid Peroxidation Following Infusion of Tert-Butyl Hydroperoxide and ADP-Iron Complex in Perfused Rat Livers.

Masuda¹,

Yamamori²

1991

Jpn.J.Pharmacol

View full text Add to dashboard Cite

ABSTRACT-Lipid peroxidation was assessed histologically and biochemically in hemoglobin-free perfused rat livers using two different types of stimulators. The Schiff reaction of fuchsin with cellular aldehydes was used as a histological index for lipid peroxidation. t-Butyl hydroperoxide (BHP, 0.8 mM) infusion caused a rapid and sus tained release of thiobarbituric acid reactive substances (TBARS) into the effluent perfusate for up to 60 min, which was accompanied by lactate dehydrogenase (LDH) leakage after 30 min. The Schiff positive foci were initially restricted to periportal zones and spread with time to whole areas, accompanied by periportal necrosis. Co infusion of diphenyl-p-phenylenediamine suppressed the TBARS release, with nega tive fuchsin staining, but the LDH leakage was unaffected. Under retrograde perfu sion, BHP produced pericentral staining and necrosis. With 2.5 mM ADP-100'U M Fe3+, little TBARS was released up to 60 min, even though the hepatic TBARS levels increased considerably by this time. By 90 min, marked TBARS release occurred, but LDH leakage remained low. Irrespective of the direction of perfusion, pericentral hepatocytes became Schiff positive after 30 min. The fuchsin staining method may be useful for detecting peroxidized zones of the liver lobules.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Histological Detection of Lipid Peroxidation Following Infusion of Tert-Butyl Hydroperoxide and ADP-Iron Complex in Perfused Rat Livers.

Masuda¹,

Yamamori²

1991

Jpn.J.Pharmacol

View full text Add to dashboard Cite

show abstract

“…By reduction of hydroperoxides to the corresponding alcohols, these enzymes can prevent the production of reactive oxygen radicals and thus may contribute to the protection of the Selenium in Biology 851 Ursini et al, 1982;Brigelius-Flohé et al, 1994 Plasma GPx (pGPx, GPx-3) Takahashi et al, 1987 Gastrointestinal GPx (GI-GPx, GPx-GI, GPx-2) Chu et al, 1993 Iodothyronine deiodinases 5Ј-deiodinase, type I (5ЈDI) Behne et al, 1990;Arthur et al, 1990 5Ј-deiodinase Lee et al, 1999;Watabe et al, 1999;Miranda-Vizuete et al, 1999;Gasdaska et al, 1999 Thioredoxin reductase homologs (SelZf1; SelZf2) Lescure et al, 1999 Selenophosphate synthetase-2 Guimaraes et al, 1996 Functionally undefined 15 kDa selenoprotein of T cells Gladyshev et al, 1998 Selenoprotein P 10 (SelP) Motsenbocker and Tappel organism's macromolecules and biomembranes against oxidation (Sies et al, 1972;Flohé, 1989;Ursini et al, 1995). The role of the cytoplasmic GPx as an 'emergency enzyme' to fight oxidative stress was verified by reverse genetics (Ho et al, 1997;Cheng et al, 1998;de Haan et al, 1998;Fu et al, 1999;Jaeschke et al, 1999) and, in this role, cGPx cannot be substituted by any of the other selenoproteins.…”

Section: Metabolic Function Of Mammalian Selenoproteinsmentioning

confidence: 99%

“…The link between nutritional science and enzymology was, however, established appreciably later when Flohé et al (1973), intrigued by a preliminary report from Hoekstra's group (Rotruck et al, 1972), identified selenium as a stoichiometric, covalently bound component of glutathione peroxidase (GPx), an enzyme previously demonstrated to dominate mammalian hydroperoxide metabolism (Sies et al, 1972). Selenium proved to be present in this enzyme as a selenocysteine residue (Forstrom et al, 1978, Wendel et al, 1978 that is integrated into the amino acid chain (Günzler et al, 1984).…”

Section: Introduction: Some Historical Landmarksmentioning

confidence: 99%

Selenium in Biology: Facts and Medical Perspectives

Köhrl¹,

Brigelius‐Flohé²,

Böck³

et al. 2000

Biological Chemistry

Self Cite

247

143

View full text Add to dashboard Cite

Several decades after the discovery of selenium as an essential trace element in vertebrates approximately 20 eukaryotic and more than 15 prokaryotic selenoproteins containing the 21 st proteinogenic amino acid, selenocysteine, have been identified, partially characterized or cloned from several species. Many of these proteins are involved in redox reactions with selenocysteine acting as an essential component of the catalytic cycle. Enzyme activities have been assigned to the glutathione peroxidase family, to the thioredoxin reductases, which were recently identified as selenoproteins, to the iodothyronine deiodinases, which metabolize thyroid hormones, and to the selenophosphate synthetase 2, which is involved in selenoprotein biosynthesis. Prokaryotic selenoproteins catalyze redox reactions and formation of selenoethers in (stress-induced) metabolism and energy production of E. coli, of the clostridial cluster XI and of other prokaryotes. Apart from the specific and complex biosynthesis of selenocysteine, selenium also reversibly binds to proteins, is incorporated into selenomethionine in bacteria, yeast and higher plants, or posttranslationally modifies a catalytically essential cysteine residue of CO dehydrogenase. Expression of individual eukaryotic selenoproteins exhibits high tissue specificity, depends on selenium availability, in some cases is regulated by hormones, and if impaired contributes to several pathological conditions. Disturbance of selenoprotein expression or function is associated with deficiency syndromes (Keshan and Kashin-Beck disease), might contribute to tumorigenesis and atherosclerosis, is altered in several bacterial and viral infections, and leads to infertility in male rodents.

show abstract

Organic Peroxides

Muianga,

Lasee

2024

Patty's Toxicology

View full text Add to dashboard Cite

Organic peroxides (ROOR′), solid or liquid with the bivalent OO structure. Relatively unstable and highly reactive molecules due to the presence of an oxygen–oxygen linkage. The oxygen–oxygen bond may be cleaved to form highly reactive free radicals and react with many substances (e.g., metals, acids, and bases). ROORs are used in plastics, rubbers, and many industries as initiators, accelerators, promoters, catalysts, activators, and more. Major concerns with ROORs are associated with fires, explosions, and corrosiveness. Reactive oxygen species and free radicals may lead to DNA damage and mutagenesis. Recent work from government agents, academic research laboratories, and manufacturing organizations have focused on physical hazards classification and categorization; and producing best practice protocols for safe handling and storage, control of temperatures, self‐accelerating decomposition temperatures, spills cleaning, disposal, and treatment of ROORs residues. These efforts allow compliance with countries and international regulations. Potential health hazards are associated with eye and skin contact, inhalation, and ingestion. Acute exposure may lead to irritation, allergic response, and potential damage to the eye and skin. As with any irritant or corrosive, dose is a critical consideration with respect to understanding the risk. Chronic exposures, mostly understood in animals but there are human data, can cause a myriad of effects. These may range from respiratory illnesses, to liver, kidney damage, and cancer. The manufacturer's SDS of individual chemical may provide toxicity information. This chapter summarizes chemical‐specific toxicity information on 77 organic peroxide compounds grouped in eight physical hazard categories. NIOSH has fully validated the method for benzoyl peroxide. NTP has released a report on the toxic effects of t ‐butyl perbenzoate and it deserves to be considered. Exposure assessment methods when available were presented. Future research needs to focus on epidemiological and toxicological studies of ROORs.

show abstract

Oxidation in the NADP system and release of GSSG from hemoglobin‐free perfused rat liver during peroxidatic oxidation of glutathione by hydroperoxides

Cited by 248 publications

References 16 publications

Histological Detection of Lipid Peroxidation Following Infusion of Tert-Butyl Hydroperoxide and ADP-Iron Complex in Perfused Rat Livers.

Histological Detection of Lipid Peroxidation Following Infusion of Tert-Butyl Hydroperoxide and ADP-Iron Complex in Perfused Rat Livers.

Selenium in Biology: Facts and Medical Perspectives

Organic Peroxides

Contact Info

Product

Resources

About