Oxidative and pre-inflammatory stress in wedge resection of pulmonary parenchyma using the radiofrequency ablation technique in a swine model

Abstract

BackgroundRadiofrequency ablation (RFA) is a thermal energy delivery system used for coagulative cellular destruction of small tumors through percutaneous or intraoperative application of its needle electrode to the target area, and for assisting partial resection of liver and kidney. We tried to evaluate the regional oxidative and pre-inflammatory stress of RFA-assisted wedge lung resection, by measuring the MDA and tumor Necrosis Factor Alpha (TNF-α) concentration in the resected lung tissue of a swine model.MethodFourteen white male swines, divided in two groups, the RFA-group and the control group (C-group) underwent a small left thoracotomy and wedge lung resection of the lingula. The wedge resection in the RFA-group was performed using the RFA technique whereas in C-group the simple "cut and sew" method was performed. We measured the malondialdehyde (MDA) and TNF-α concentration in the resected lung tissue of both groups.ResultsIn C-group the MDA mean deviation rate was 113 ± 42.6 whereas in RFA-group the MDA mean deviation rate was significantly higher 353 ± 184 (p = 0.006). A statistically significant increase in TNF-α levels was also observed in the RFA-group (5.25 ± 1.36) compared to C-group (mean ± SD = 8.48 ± 2.82) (p = 0.006).ConclusionOur data indicate that RFA-assisted wedge lung resection in a swine model increases regional MDA and TNF-a factors affecting by this oxidative and pre-inflammatory stress of the procedure. Although RFA-assisted liver resection can be well tolerated in humans, the possible use of this method to the lung has to be further investigated in terms of regional and systemic reactions and the feasibility of performing larger lung resections.

The Role of Thoracotomy in the Postoperative Inflammatory Mechanism

“…In an animal study, a statistically significant increase in regional oxidative stress was observed only when the pulmonary resection by radiofrequency ablation technique was used [11]. In the present study, a benign or malignant indication of thoracotomy did not influence the amount of inflammation.…”

A case of thoracoabdominal splenosis

“…Splenosis is most prevalent in the abdomen and, much less commonly, in the thorax [6]; here, we report a case of disseminated asymptomatic splenosis localized to the left pleural cavity and upper right and left quadrants in a 38-year-old male with a remote history of motor vehicle trauma.…”

“…The mean time between the injury responsible for splenosis and the actual diagnosis is about 2 decades [5]. Nevertheless, there are reports of splenosis presenting as persistent chest pain [6], [7], hemoptysis [8], and bowel obstruction [9]. Although diagnosis via biopsy is the gold standard, imaging informed by a relevant patient history including splenectomy or prior trauma to the spleen, is still acceptable [10].…”

Protective effect of extracorporeal membrane pulmonary oxygenation combined with cardiopulmonary resuscitation on post-resuscitation lung injury

“…Lipid peroxidation is a mature mechanism of cell damage in plants and animals, and it is used as an indicator of oxidative stress in cells and tissues. [28] Several experiments have shown that serum examination of MDA and SOD can refl ect the degree of oxidative damage to tissues. [29,30] MPO is an important enzyme released by neutrophils, and promotes the formation of hypochlorous acid, a powerful oxidant associated with bactericidal effects and tissue destruction through induction of necrosis and apoptosis.…”