Oxidative and reductive acetyl CoA/carbon monoxide dehydrogenase pathway in Desulfobacterium autotrophicum

Schauder, Rolf; Preuß, Andrea; Jetten, Mike S. M.; Fuchs, Georg

doi:10.1007/bf00444674

Cited by 117 publications

(73 citation statements)

References 38 publications

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“…Such organisms include acetogens, methanogens, sulfate reducing bacteria (SRB), ammoniaoxidizing planctomycetes, an autotrophic archaeal SRB, Archaeoglobales (Berg, 2011), anaerobic facultative autotrophs (Schauder et al, 1988) and, reversibly, with heterotrophs using carbon monoxide dehydrogenase (CODH) and acetyl-CoA synthase to oxidize acetyl-CoA (Rabus et al, 2006). Callaghan et al (2012) have also postulated that a reversed Wood-Ljungdahl pathway is carried out during the complete oxidation of alkanes under anaerobic conditions.…”

Section: Geochemical Characteristicsmentioning

confidence: 99%

A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

et al. 2015

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Subsurface microbial communities comprise a significant fraction of the global prokaryotic biomass; however, the carbon metabolisms that support the deep biosphere have been relatively unexplored. In order to determine the predominant carbon metabolisms within a 3-km deep fracture fluid system accessed via the Tau Tona gold mine (Witwatersrand Basin, South Africa), metagenomic and thermodynamic analyses were combined. Within our system of study, the energy-conserving reductive acetyl-CoA (Wood-Ljungdahl) pathway was found to be the most abundant carbon fixation pathway identified in the metagenome. Carbon monoxide dehydrogenase genes that have the potential to participate in (1) both autotrophic and heterotrophic metabolisms through the reversible oxidization of CO and subsequent transfer of electrons for sulfate reduction, (2) direct utilization of H 2 and (3) methanogenesis were identified. The most abundant members of the metagenome belonged to Euryarchaeota (22%) and Firmicutes (57%)-by far, the highest relative abundance of Euryarchaeota yet reported from deep fracture fluids in South Africa and one of only five Firmicutes-dominated deep fracture fluids identified in the region. Importantly, by combining the metagenomics data and thermodynamic modeling of this study with previously published isotopic and community composition data from the South African subsurface, we are able to demonstrate that Firmicutes-dominated communities are associated with a particular hydrogeologic environment, specifically the older, more saline and more reducing waters.

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Section: Geochemical Characteristicsmentioning

confidence: 99%

A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

et al. 2015

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“…Two eastern branch genes, one encoding a formate-tetrahydrofolate ligase (Peg.3045) and one encoding a methylenetetrahydrofolate dehydrogenase (NADP þ )/methenyltetrahydrofolate cyclohydrolase (Peg.2652), are likely involved in reducing CO 2 to a methyl group. It should be noted that some sulfate-reducing organisms and aceticlastic methanogens could run the WL pathway in reverse (Schauder et al, 1988;Hattori et al, 2005). We hypothesized that Mcas may be capable of reversing the WL pathway and generating metabolic energy by oxidizing acetate to CO 2 .…”

Section: Carbon Fixationmentioning

confidence: 99%

Genomic insights into the uncultured genus ‘Candidatus Magnetobacterium’ in the phylum Nitrospirae

et al. 2014

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Magnetotactic bacteria (MTB) of the genus 'Candidatus Magnetobacterium' in phylum Nitrospirae are of great interest because of the formation of hundreds of bullet-shaped magnetite magnetosomes in multiple bundles of chains per cell. These bacteria are worldwide distributed in aquatic environments and have important roles in the biogeochemical cycles of iron and sulfur. However, except for a few short genomic fragments, no genome data are available for this ecologically important genus, and little is known about their metabolic capacity owing to the lack of pure cultures. Here we report the first draft genome sequence of 3.42 Mb from an uncultivated strain tentatively named 'Ca. Magnetobacterium casensis' isolated from Lake Miyun, China. The genome sequence indicates an autotrophic lifestyle using the Wood-Ljungdahl pathway for CO 2 fixation, which has not been described in any previously known MTB or Nitrospirae organisms. Pathways involved in the denitrification, sulfur oxidation and sulfate reduction have been predicted, indicating its considerable capacity for adaptation to variable geochemical conditions and roles in local biogeochemical cycles. Moreover, we have identified a complete magnetosome gene island containing mam, mad and a set of novel genes (named as man genes) putatively responsible for the formation of bullet-shaped magnetite magnetosomes and the arrangement of multiple magnetosome chains. This first comprehensive genomic analysis sheds light on the physiology, ecology and biomineralization of the poorly understood 'Ca. Magnetobacterium' genus.

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“…The acetogens are known to use the reductive CODH/ACS pathway for both dissimilation and assimilation. In addition to the acetogens, several anaerobic microbes such as sulfate reducers, methanogens, and syntrophic propionate-oxidizing bacteria employ an analogous pathway 52,55,56,74,78) . A principle of the reductive CODH/ACS pathway is the reduction of 2 moles of CO2 to 1 mol of acetate by using 8 reducing equivalents [H] (Fig.…”

Section: Isolation and Characterization Of The Syntrophic Acetate-oximentioning

confidence: 99%

“…Although the reductive CODH/ACS pathway can generate energy by reducing CO2 to acetate, reversal of the pathway, here termed the oxidative CODH/ACS pathway, is operated by several anaerobic microbes including sulfate reducers 55,56,66) and methanogens 15,76) . In the oxidative CODH/ACS pathway, theoretically 1 mol of acetate is oxidized to produce 8[H] and 2 moles of CO 2 (opposite direction in Fig.…”

Section: Isolation and Characterization Of The Syntrophic Acetate-oximentioning

confidence: 99%

“…M. thermoacetica and A. woodii) harbor the reductive CODH/ACS pathway, it has not been reported whether they can operate it in the reverse direction for syntrophic acetate oxidation. Likewise, there are no reports that acetate-oxidizing sulfate reducers (e.g., Desulfotomaculum acetoxidans and Desulfobacterium autotrophicum) can oxidize acetate in a syntrophic relationship with hydrogenotrophic methanogens, although they harbor the oxidative CODH/ACS pathway 55,56,66) . Similar relationships are also likely to be found between the other syntrophic acetate oxidizers (e.g., T. lettingae and G. sulfurreducens) and their relatives (e.g., Thermotoga elfii and Geobacter metallireducens).…”

Section: Isolation and Characterization Of The Syntrophic Acetate-oximentioning

confidence: 99%

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Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments

2008

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Acetate is one of the most important intermediates for methanogenesis in the anaerobic mineralization of organic materials. Methanogenic acetate degradation is carried out by either an aceticlastic reaction or an anaerobic acetateoxidizing reaction. In contrast to the former reaction, the latter is energetically extremely unfavorable. However, the oxidation of acetate can occur with syntrophic interaction between certain bacteria and methanogenic archaea. The bacteria, namely syntrophic acetate-oxidizing bacteria, can oxidize acetate to produce hydrogen/CO2 only when their products are subsequently utilized by the hydrogen-scavenging methanogens. Surprisingly, some of these bacteria can also axenically grow on hydrogen/CO2 to produce acetate. This means that the bacteria can utilize both substrates and products reversibly. This review describes current studies of these curious and fascinating microbes.

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Oxidative and reductive acetyl CoA/carbon monoxide dehydrogenase pathway in Desulfobacterium autotrophicum

Cited by 117 publications

References 38 publications

A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

Genomic insights into the uncultured genus ‘Candidatus Magnetobacterium’ in the phylum Nitrospirae

Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments

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