Oxidative-antioxidative status and hepatic and renal factors following melatonin administration in castrated and intact dogs

Abstract

BackgroundsMelatonin has significant antioxidant and hepatoprotective effects in normal and oxidative stress conditions. The aim of the present study was to assess the effects of melatonin on antioxidant, hepatic, and renal factors in intact and castrated dogs. Twenty male mixed-breed adult dogs were aligned in an experimental randomized and controlled trial. The dogs were randomly divided into four equal groups: melatonin, castrated, castrated and melatonin, and control. They were treated with melatonin (0.3 mg/Kg, once daily, orally) immediately after the castration for 1 month and their blood samples were collected weekly from 2 days after treatment with melatonin.ResultsTreating castrated dogs with melatonin increased the level of glutathione peroxidase, superoxide dismutase, and catalase compared with that of the control and castrated groups. The malondialdehyde level increased significantly following castration. Melatonin treatment decreased malondialdehyde concentration in the castrated dogs. Castration increased the level of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase significantly in comparison with that of the control group. Treating the castrated dogs with melatonin decreased significantly liver enzymes compared with those of the castrated dogs. Blood urea nitrogen and creatinine levels increased in the castrated dogs in comparison with that of the control group.ConclusionsThe administration of melatonin in castrated dogs increased antioxidant activity and decreased oxidation products, compared with those of the castrated and untreated dogs, without adverse effects on liver enzymes and kidney function.

Surgical castration versus chemical castration in donkeys: response of stress, lipid profile and redox potential biomarkers

“…The elevation in serum TPX of donkeys submitted to the surgical castration compared to those submitted to the chemical castration at day 45 is in parallel with the disturbance in the redox homeostasis of surgically castrated dogs [15]. The surgical castration in pigs encouraged lipid peroxidation in the pork sausage more than that found in the immunological one [65] due to the ability of the surgical castration to increase the polyunsaturated fatty acids, which subsequently enhances the susceptibility of cells to free radical attack [66,67].…”

“…Stressful conditions like castration cause imbalance between oxidants and antioxidants in favor of oxidants at the cellular and individual levels creating status of oxidative stress [19]. The greater the oxidative stress, the more severe the cellular damage would be during the surgery which may cause poor post-operation outcomes [15].…”

“…Surgical castration resulted in oxidative stress in the blood of Wistar/ST rats and mixed-breed dogs [ 15 , 16 ], also CaCl 2 castration caused peroxidative injury in the testicular tissues of albino rats, dogs, and black Bengal goats [ 3 – 6 ]. These discrepancies add fuel to our study in the light of species-related differential response.…”

The Effects of Melatonin Treatment on Oxidative Stress Induced by Ovariohysterectomy in Dogs

“…Therefore, the researchers should nd a way that minimizes the harmful effects of free radicals. Melatonin has been shown in numerous studies to have antioxidant properties [17,29]. Mogheiseh et al (2019) found that dogs were under oxidative stress after castration and that melatonin improved the antioxidant status of intact and castrated dogs [17].…”

“…As in our previous studies, melatonin was administrated daily just before and after spaying. Also, since the effect of anesthesia on oxidative stress had to be examined in comparison with other groups [16][17][18][19], the dogs were divided into ve equal groups (n = 5) in a case-control randomized study (Fig. 1).…”