Oxidative Assimilation of Lactic Acid by Escherichia coli.

Clifton, C. E.; Logan, William A.T.

doi:10.3181/00379727-38-9957

Cited by 11 publications

(3 citation statements)

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“…The oxidative assimilation of lactic acid in washed suspensions and in cultures of Escherichia coli has recently been described by Clifton and Logan (1938). The oxygen uptake in a M/15 phosphate buffer of pH 7.1, proceeds at a rapid and quite constant rate until approximately two-thirds of the oxygen required for complete combustion has been consumed.…”

Section: E Clifon and W A Loganmentioning

confidence: 99%

On the Relation between Assimilation and Respiration in Suspensions and in Cultures of Escherichia coli

Clifton

Logan

1939

J Bacteriol

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Until recently, it has been generally assumed that the conversion under highly aerobic conditions of relatively simple, non-nitrogenous compounds by washed suspensions of bacteria is limited to an oxidation of the substrate to carbon dioxide and water. Barker (1936), Geisberger (1936) and Clifton (1937) have shown that the oxidation does not proceed to completion, but that instead, a portion of the substrate is apparently assimilated by the cells. For example, the oxidative assimilation of acetate and of butyrate by Pseudomonas calco-acetica may be represented as 2CH3COOH + 302 = (CH20) + 3 C02 + 3H20, (1) and 2C4H802+ 702 = 3 (CH20) + 5 C02 + 5H20 (2) These equations indicate that approximately three-fourths of the oxygen required for the complete combustion of these substrates is consumed, with the production of carbon dioxide, water and a substance, or substances, having the empirical composition of a carbohydrate. The oxidative assimilation of butyrate is of particular interest since the respiratory quotient (R.Q.) during the stage of rapid oxidation is approximately 0.68 while the theoretical R.Q. for complete combustion is 0.8. The rate of oxygen consumption abruptly decreases to a level near that of the control by the 523

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Section: E Clifon and W A Loganmentioning

confidence: 99%

On the Relation between Assimilation and Respiration in Suspensions and in Cultures of Escherichia coli

Clifton

Logan

1939

J Bacteriol

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“…The role of oxidative phosphorylation and of the energy-rich phosphate bond in energy transfer, however, was not known in 1937. The studies on oxidative assimilation were continued at Stanford, Clifton & Logan (25,26) reporting that about the same amount of assimilation was noted in actively proliferating cultures as in washed suspensions of E. coli. Pickett & Clifton (27) reported that dinitrophenol blocked assimilation by yeast but oxidation did not go to completion, the glucose normally assimi lated being fermented in the presence of the poison.…”

Section: Cliftonmentioning

confidence: 99%

Microbiology—Past, Present, and Future

Clifton

1966

Annu. Rev. Microbiol.

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“…It has been known for more than 40 years (2, 4) that when a microbial cell suspension has metabolized a substrate completely and consumption of oxygen has ceased, the measured uptake of 02 faDs far short of that calculated for combustion to carbon dioxide and water. It was proposed that only a proportion of the substrate is oxidized to completion, the remainder being "assimilated" as intracellular metabolites.…”

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confidence: 99%