Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix

Abstract

Free radicals that induced lipid peroxidation and DNA damage have been implicated in many diseases including cancer. Cellular antioxidant defense plays an important role in neoplastic disease to counteract oxidative damage. This study aims to investigate the status of oxidative damage by measuring plasma malondialdehyde (MDA) level and urinary 8-hydroxydeoxyguanosine (8-OHdG), and the level of antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase in patients with cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of the cervix. Urinary 8-OHdG was measured by an enzyme-linked immunosorbent assay kit. MDA and antioxidant enzyme activities were determined by high-performance liquid chromatography and spectrophotometry, respectively. Eighty patients with CIN and SCC of the cervix were recruited and compared with normal controls. Urinary 8-OHdG/creatinine ratio did not show any significant changes in any disease status studied as compared with controls (P=0.803). Plasma MDA was found to be increased in CIN and SCC patients when compared with controls (P=0.002). Glutathione peroxidase activity was increased (P=0.0001) whereas superoxide dismutase and catalase activity was decreased (P=0.019 and 0.0001, respectively) in both CIN and SCC patients when compared with controls. Urinary 8-OHdG may not be a good marker for enhanced oxidative stress in cervical cancer. Oxidative damage as demonstrated by the level of MDA is markedly increased in CIN and SCC patients with changes of enzymatic antioxidants observed.

Role and predictive strength of transglutaminase type 2 expression in premalignant lesions of the cervix

“…43 Oxidative damage is markedly increased in CIN. 44 It is possible that TG2 shuttles in the nucleus to protect the cells by modulating hypoxia-mediated transcriptional events, possibly by attenuating HIF activation of pro-cell death genes as observed during ischemic stroke. 45 The purpose of our study has been to clarify the role of TG2 during the evolution of CIN lesions.…”

The morpho-functional assessment of plasmonic photothermal therapy effects on transplanted liver tumor

“…A number of authors [19][20][21] have shown that excessive formation of free radicals and inactivation of antioxidant enzyme on the background of antitumor laser therapy ensures the accumulation of toxic products of oxidative stress intensifying the severity of endogenous tumor intoxication. Secondary metabolic disorders are accompanied by toxic damage of cell membranes of various internal organs and tissues, which is often the limiting factor for further treatment.…”

Serum levels of soluble interleukin-2 receptor in association with oxidative stress index in patients with different types of HPV

“…Oxidative status has been reported altered in blood of patients with cervical intraepithelial neoplasia (CIN). Studies on serum of patients with CIN and carcinoma of the cervix showed alterations in lipid peroxidation and impairment of antioxidant systems, either enzymatic or not [24,25]. Additionally, other blood indicators of oxidative stress such as TBARS, ALA-D reactivation index, and vitamin C content were reported altered (increased or decreased) in both low-grade and high-grade SIL, as well as cervical cancer [26].…”