Oxidative Damage Induced by Heat Stress Could be Relieved by Nitric Oxide in Trichoderma harzianum LTR-2

Yu, Yang; Zhou, Yang; Guo, Kai; Li, Zhe; Zhou, Hongzi; Wei, Yanli; Li, Jishun; Zhang, Xinjian; Harvey, Paul R.; He-tong, Yang

doi:10.1007/s00284-014-0764-8

Cited by 17 publications

(10 citation statements)

References 16 publications

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“…In Candida albicans, NO is involved in oxidative stress and azoles (31). In Trichoderma harzianum, NO can alleviate oxidative damage (32), and this effect was also demonstrated in P. eryngii var. tuoliensis (16).…”

Section: Discussionmentioning

confidence: 92%

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO alleviated oxidative damage to P. ostreatus mycelia by inhibiting the protein and gene expression of aconitase (ACO), and additional studies found that the overexpression and interference of aco could affect the content of citric acid (CA). Furthermore, the addition of exogenous CA can induce alternative oxidase (aox) gene expression under heat stress, reduce the content of H 2 O 2 in mycelium, and consequently protect the mycelia under heat stress. An additional analysis focused on the function of the aox gene in the heat stress response of mycelia. The results show that the colony diameter of the aox overexpression (OE-aox) strains was significantly larger than that of the wild-type (WT) strain under heat stress (32°C). In addition, the mycelia of OEaox strains showed significantly enhanced tolerance to H 2 O 2 . In conclusion, this study demonstrates that NO can affect CA accumulation by regulating aco gene and ACO protein expression and that CA can induce aox gene expression and thereby be a response to heat stress. IMPORTANCE Heat stress is one of the abiotic stresses that affect the growth and development of edible fungi. Our previous study found that exogenous NO had a protective effect on mycelia under heat stress. However, its regulatory mechanism had not been elucidated. In this study, we found that NO altered the respiratory pathway of mycelia under heat stress by regulating aco. The results have enhanced our understanding of NO signaling pathways in P. ostreatus.

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Section: Discussionmentioning

confidence: 92%

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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“…This result is consistent with many previous studies 28 . Yu reported that NO can enhance the activity of NOS and NR to reduce oxidative damage and protect the mycelia of T. harzianum from HS 47 . The mechanism of NO enhancement of the response of G. oregonense to HS has been revealed by RNA-Seq analysis.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress

Chen

Wang

et al. 2017

Sci Rep

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Ganoderma is well known for its use in traditional Chinese medicine and is widely cultivated in China, Korea, and Japan. Increased temperatures associated with global warming are negatively influencing the growth and development of Ganoderma. Nitric oxide is reported to play an important role in alleviating fungal heat stress (HS). However, the transcriptional profiling of Ganoderma oregonense in response to HS, as well as the transcriptional response regulated by NO to cope with HS has not been reported. We used RNA-Seq technology to generate large-scale transcriptome data from G. oregonense mycelia subjected to HS (32 °C) and exposed to concentrations of exogenous NO. The results showed that heat shock proteins (HSPs), “probable stress-induced proteins”, and unigenes involved in “D-amino-acid oxidase activity” and “oxidoreductase activity” were significantly up-regulated in G. oregonense subjected to HS (P < 0.05). The significantly up-regulated HSPs, “monooxygenases”, “alcohol dehydrogenase”, and “FAD/NAD(P)-binding domain-containing proteins” (P < 0.05) regulated by exogenous NO may play important roles in the enhanced HS tolerance of G. oregonense. These results provide insights into the transcriptional response of G. oregonense to HS and the mechanism by which NO enhances the HS tolerance of fungi at the gene expression level.

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“…Meanwhile, as a physiological regulator, NO signaling is involved in mediating stomatal closure (Noelia et al, 2015 ; Shi K. et al, 2015 ; Chen et al, 2016 ), pollen tube growth (Wang et al, 2009 ). Also, NO plays an essential role in plant disease resistance (Rasul et al, 2012 ; Kovacs et al, 2015 ) and responses to various abiotic stresses such as cold (Fan et al, 2015 ), heat (Yu et al, 2015 ), salt (Liu W. et al, 2015 ), drought (Shan et al, 2015 ), UV-B (Esringu et al, 2015 ) and heavy metal (Alemayehu et al, 2015 ; Chen et al, 2015 ; Kaur et al, 2015 ). These studies have paved the way to understand the signaling roles of NO which may affect cell metabolism, cellular redox balance and gene expression in plants.…”

Section: Crosstalk Between H 2 O 2 mentioning

confidence: 99%

Hydrogen Peroxide Signaling in Plant Development and Abiotic Responses: Crosstalk with Nitric Oxide and Calcium

2016

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Hydrogen peroxide (H2O2), as a reactive oxygen species, is widely generated in many biological systems. It has been considered as an important signaling molecule that mediates various physiological and biochemical processes in plants. Normal metabolism in plant cells results in H2O2 generation, from a variety of sources. Also, it is now clear that nitric oxide (NO) and calcium (Ca2+) function as signaling molecules in plants. Both H2O2 and NO are involved in plant development and abiotic responses. A wide range of evidences suggest that NO could be generated under similar stress conditions and with similar kinetics as H2O2. The interplay between H2O2 and NO has important functional implications to modulate transduction processes in plants. Moreover, close interaction also exists between H2O2 and Ca2+ in response to development and abiotic stresses in plants. Cellular responses to H2O2 and Ca2+ signaling systems are complex. There is quite a bit of interaction between H2O2 and Ca2+ signaling in responses to several stimuli. This review aims to introduce these evidences in our understanding of the crosstalk among H2O2, NO, and Ca2+ signaling which regulates plant growth and development, and other cellular and physiological responses to abiotic stresses.

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Oxidative Damage Induced by Heat Stress Could be Relieved by Nitric Oxide in Trichoderma harzianum LTR-2

Cited by 17 publications

References 16 publications

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress

Hydrogen Peroxide Signaling in Plant Development and Abiotic Responses: Crosstalk with Nitric Oxide and Calcium

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