Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Abstract

The impact of metals bioaccumulation in marine organisms is a subject of intense investigation. This study was designed to determine the association between oxidative stress induced by seawater enriched with trace metals and protein synthesis using as a model the mussels Mytilus galloprovincialis. Mussels were exposed to 40 μg/L Cu, 30 μg/L Hg, or 100 μg/L Cd for 5 and 15 days, and the pollution effect was evaluated by measuring established oxidative biomarkers. The results showed damage on the protein synthesis machine integrity and specifically on translation factors and ribosomal proteins expression and modifications. The exposure of mussels to all metals caused oxidative damage that was milder in the cases of Cu and Hg and more pronounced for Cd. However, after prolonged exposure of mussels to Cd (15 days), the effects receded. These changes that perturb protein biosynthesis can serve as a great tool for elucidating the mechanisms of toxicity and could be integrated in biomonitoring programs.

Metal Bioaccumulation and Oxidative Stress in Ulva laetevirens in the Venice Lagoon: Early Warning Biomarker for Metal Bioaccumulation

“…Similarly, the pro-oxidant levels accompanied the enhanced accumulation of metal(loid)'s As and Pb in the digestive gland of the Manila clam Ruditapes philippinarum collected in a contaminated site in Tagus estuary [Portugal] [72]. The mussels Mytilus galloprovincialis exposed to seawater enriched with trace metals (Cu, Hg, and Cd) caused oxidative damage that was milder in the cases of Cu and Hg and more pronounced for Cd [73]. Significant different values of MDA were recorded after 15 days of exposure to Cd +2 [74].…”

Oxidative response to accumulation of trace metals in tissue of two bivalves, the Asian green mussel Perna viridis and the blood cockle Tegillarca granosa, living in Pattani Bay, Thailand

“…Bivalves are known as bioindicator organisms for monitoring chemical pollutants, including trace metals, due to their ability to accumulate and concentrate pollutants in their tissues (Rouane-Hacene et al 2015). Bivalves, especially | 33 BIVALVE OXIDATIVE RESPONSE TO TRACE METALS filter-feeding ones, can directly accumulate trace metals from their food via sediment and water (Kournouto et al 2020). Trace metals in water, sediment, and their tissue can be related to physiological changes in aquatic organisms and influence the organisms' health (Deb and Fukushima 1999;Vlahogianni and Valavanidis 2007;Tanhan et al 2023a;Zhan et al 2023).…”

“…Exposure to a variety of heavy metals can lead to the disruption of redox systems, both directly via the Fenton reaction and indirectly through depletion of antioxidant properties (Kournouto et al 2020). Incremental lipid peroxidation, superoxide radicals, lysosomal membrane destabilization, macromolecule damage, and depletion of reduced glutathione (GSH), glutathione peroxidase (GPx: enzyme code 1.11.1.9), catalase (CAT: 1.11.1.6), and superoxide dismutase (SOD: 1.15.1.1) activities are involved (Kournouto et al 2020). Alteration of gene expression has been characterized, and the genotoxic effect of heavy metals has been indicated (Bolognesi et al 1999).…”

“…Bivalves, especially filter‐feeding ones, can directly accumulate trace metals from their food via sediment and water (Kournouto et al. 2020). Trace metals in water, sediment, and their tissue can be related to physiological changes in aquatic organisms and influence the organisms' health (Deb and Fukushima 1999; Vlahogianni and Valavanidis 2007; Tanhan et al.…”

Nuclear Factor Erythroid-2-Related Factor 2 (Nrf2) Signalling Pathway and Apoptosis Impact on the Effect of Tetramethylpyrazine against H2O2-Damage in Human Hepatic Cell Line (LO2 Cells)

“…Oxidative damage is caused by a wide range of factors including ultraviolet stress, smoking, medicine, drugs, and so on (Kournoutou et al, 2020). Oxidative stress is the result of an imbalance between Reactive Oxygen Species (ROS) and the antioxidant defense system (LeBel et al, 1992).…”