Oxidative damage to the hepatocellular proteins after chronic ethanol intake in the rat

Abraham, Premila; Wilfred, G.; Ramakrishna, B. S.

doi:10.1016/s0009-8981(02)00279-6

Cited by 40 publications

(35 citation statements)

References 53 publications

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“…Reseachers observed that zinc deficiency was not only related with the ethanol intake directly but also dependent on the level of the liver damage [3]. The spectrum of alcoholic liver disease includes fatty liver, alcoholic hepatitis, fibrosis and cirrhosis [4]. Based on the histological findings of our experimental model, early phase of the liver damage might cause the insignificant results for zinc levels in the EtOH group.…”

Section: Discussionmentioning

confidence: 75%

“…Based on the histological findings of our experimental model, early phase of the liver damage might cause the insignificant results for zinc levels in the EtOH group. Proteins, especially thiol containing proteins are major targets for free radicals [4]. Advanced oxidation protein products (AOPP) was defined as protein products containing crosslinked dityrosine bonds [11].…”

Section: Discussionmentioning

confidence: 99%

“…Zinc Sulfate (ZnSO 4 .7H 2 O) was obtained from Sigma Ò (St Louis, MO, USA). All other reagents and chemicals were of analytical grade.…”

Section: Chemicalsmentioning

confidence: 99%

“…Damaged proteins in the ethanol treated rats may have altered the metabolism and the structure of subcellular organelles of the liver and contribute to fat accumulation. Protein oxidation is thought as a modification due to ethanol toxicity [4]. Zinc is known as an essential trace element necessary for protein metabolism.…”

Section: Introductionmentioning

confidence: 99%

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The effect of zinc on ethanol-induced oxidative stress in rat liver

Caglar¹,

Bi̇lgi̇han²,

Turkcu³

et al. 2012

Turk J Bioch

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Aim Zinc is known as an essential trace element and has antioxidant functions. This study was planned to investigate the effects of zinc on oxidative stress induced by ethanol in the rat liver tissue. Methods: Thirty-nine male rats were divided into four groups as control, ethanol (EtOH), zinc (Zn), ethanol plus zinc (EtOH+Zn). The control group (n=10) were injected intraperitoneally (i.p.) with 0.9% saline, EtOH group (n=10) with 2g kg/day ethanol, Zn group (n=10) received orally, ZnSO 4 .7H 2 O at a dose of 7 mg kg/day and EtOH+Zn group (n=9) received zinc (orally) and ethanol (i.p.). On the 13th day, rats were euthanized. Liver tissues were removed and malondialdehyde (MDA), advanced oxidation protein products (AOPP), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) activities were measured. Tissue zinc levels were determined. Histological analyses of liver tissue specimens were also performed. Results: In EtOH group MDA levels, GPx, SOD activities increased compared with control group. In accordance with histological findings, ethanol induced oxidative stress. But AOPP levels decreased in EtOH group due to supressive effect of ethanol on neutrophil activation and reactive oxygen species (ROS) production. In EtOH+Zn group, MDA, AOPP levels and GPx, SOD activities significantly changed. Besides, histological findings showed that zinc supplementation reduced oxidative stress. But in healthy rats MDA levels increased due to ROS mediated zinc damage. Conclusion: Zinc supplementation was found to offer protection against ethanol-induced liver damage and oxidative stress in rats. However, zinc might increase oxidative stress in healthy rats. Key Words: Ethanol, zinc; oxidative stress, antioxidant, liver Conflict of interest: Authors did not declare any conflict of interest. ÖZETAmaç: Çinko antioksidan etkileri olan esansiyel bir eser elementtir. Bu çalışma, rat karaciğerinde etanolle indüklenen oksidatif strese çinkonun etkilerini incelemek amacıyla planlanmıştır. Gereç ve Yöntemler: Otuz dokuz erkek rat 4 gruba ayrılmıştır. Kontrol, etanol (EtOH), çinko (Zn), etanol ve çinko (EtOH+Zn). Kontrol grubuna (n=10) intraperitonel (i.p.) % 0.9 tuz çözeltisi, EtOH grubuna (n=10) 2g/kg/gün etanol, Zn grubuna (n=10) oral olarak 7 mg/ kg/gün ZnSO 4 .7H 2 O ve ETOH+Zn grubuna (n=9) oral olarak çinko ve i.p. ethanol verilmiştir. Onüçüncü gün ratlar feda edilmiştir. Karaciğer dokuları çıkarılarak malondialdehit (MDA), ileri okside protein ürünleri (AOPP) düzeyleri, süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), glutatyon redüktaz (GR), ve glutatyon-S-transferaz (GST) aktiviteleri ve doku çinko düzeyleri ölçülmüştür.Karaciğer dokularının histolojik incelemeleri gerçekleştirilmiştir. Bulgular: Etanol grubunda hepatik MDA düzeyleri, GPx, SOD aktiviteleri kontrol grubuna göre artmıştır ve histolojik bulguların da desteklediği gibi etanolün oksidatif stresi artırdığını göstermektedir.Ancak EtOH grubundaki AOPP düzeylerindeki düşüş, etanolün nötro...

show abstract

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

“…Zinc Sulfate (ZnSO 4 .7H 2 O) was obtained from Sigma Ò (St Louis, MO, USA). All other reagents and chemicals were of analytical grade.…”

Section: Chemicalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of zinc on ethanol-induced oxidative stress in rat liver

Caglar¹,

Bi̇lgi̇han²,

Turkcu³

et al. 2012

Turk J Bioch

View full text Add to dashboard Cite

show abstract

“…However, antioxidant supplementation (GSH-vitamin C and E) ameliorated mucosal damages in the gastric tissues as shown in Table 2 and Figure 3. As cited in literature, protein (carbonyl) oxidation and lipid peroxidation are commonly used as biomarkers of tissue damage or oxidative stress [17,18]. In addition to these, the stomach mucosal damage caused by aspirin appears to involve oxidative stress [19].…”

Section: Discussionmentioning

confidence: 99%