Oxidative damages by cadmium and the protective effects of low-molecular-weight chitosan in the freshwater crab (Sinopotamon yangtsekiense Bott 1967)

Li, Ruijin; Zhou, Yanying; Ji, Jinfang; Wang, Lan

doi:10.1111/j.1365-2109.2010.02646.x

Cited by 28 publications

(14 citation statements)

References 29 publications

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“…However, few data are available regarding the effects of exposure to cyanobacterial neurotoxins or PSPs on ROS and T-AOC in fish gills. The current study demonstrated that aphantoxins or PSPs trigger elevations in ROS and reductions in T-AOC levels in the zebrafish gill, suggesting that these toxins induce oxidative stress by increasing the production of free radicals and decreasing the synthesis and/or increasing the use of antioxidants, which is in agreement with previous research (Li et al, , 2011.…”

Section: Changes In Ros and T-aoc In The Gills Of Aphantoxin-exposed supporting

confidence: 93%

In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill

et al. 2016

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Section: Changes In Ros and T-aoc In The Gills Of Aphantoxin-exposed supporting

confidence: 93%

In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill

et al. 2016

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“…Thus, more and more LMWCs are used as antioxidant agents. For example, LMWCs are utilized in the preservation of crab and Kamaboko gels and the preparation of edible antioxidant film [ 64 – 67 ]. In this study, the hydrolysate products of chitosan derivatives, named EG-LMWC, CM-LMWC and AE-LMWC, exhibited significant free radical scavenging activities, and the free radical scavenging activities of those chitosan derivatives were greater than commercial COS. CM-LMWC and AE-LMWC showed superior activity towards ABTS ․+ and peroxyl free radicals compared to EG-LMWC.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Chitosanase from Jelly Fig (Ficus awkeotsang Makino) Latex and Its Application in the Production of Water-Soluble Low Molecular Weight Chitosans

Chang

Lin

et al. 2016

PLoS ONE

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A chitosanase was purified from jelly fig latex by ammonium sulfate fractionation (50–80% saturation) and three successive column chromatography steps. The purified enzyme was almost homogeneous, as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gel activity staining. The molecular mass of the enzyme was 20.5 kDa. The isoelectric point (pI) was <3.5, as estimated by isoelectric focusing electrophoresis on PhastGel IEF 3-9. Using chitosan as the substrate, the optimal pH for the enzyme reaction was 4.5; the kinetic parameters Km and Vmax were 0.089 mg mL-1 and 0.69 μmol min-1 mg-1, respectively. The enzyme showed activity toward chitosan polymers which exhibited various degrees of deacetylation (21–94%). The enzyme hydrolyzed 70–84% deacetylated chitosan polymers most effectively. Substrate specificity analysis indicated that the enzyme catalyzed the hydrolysis of chitin and chitosan polymers and their derivatives. The products of the hydrolysis of chitosan polymer derivatives, ethylene glycol (EG) chitosan, carboxymethyl (CM) chitosan and aminoethyl (AE) chitosan, were low molecular weight chitosans (LMWCs); these products were referred to as EG-LMWC, CM-LMWC and AE-LMWC, respectively. The average molecular weights of EG-LMWC, CM-LMWC and AE-LMWC were 11.2, 11.2 and 8.89 kDa, respectively. All of the LMWC products exhibited free radical scavenging activities toward ABTS•+, superoxide and peroxyl radicals.

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“…Cd ions may induce the accumulation of reactive oxygen species (ROS) [22] which can lead to lipid peroxidation of membrane [23] and is harmful to the mitochondria [24]. Not only mitochondria are the site of energy production, but their roles in the regulation of apoptosis have been accepted [25].…”

Section: Introductionmentioning

confidence: 99%

Cd-Induced Apoptosis through the Mitochondrial Pathway in the Hepatopancreas of the Freshwater Crab Sinopotamon henanense

Liu

Yang

et al. 2013

PLoS ONE

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Cd is one of the most common pollutants in the environment that also induces the apoptosis. To explore the mechanism of apoptosis in the hepatopancreas, freshwater crab S . henanense were treated with 0, 3.56, 7.12, 14.25, 28.49 and 56.98 mg/L Cd for 72 h. Apoptosis was noticeable in every treatment group and necrosis was observed clearly in the high concentration Cd groups. Classical apoptotic bodies were found by transmission electronic microscopy, which revealed chromatin condensation under nuclear membrane and mitochondrial membrane rupture. An increasing number of autolysosomes, damaged rough endoplamic reticulum and Golgi complex were observed as the Cd concentration increase. Brown colored apoptotic cells were detected by the TUNEL test in all Cd-treatment groups. The apoptosis index increased following the elevation of Cd concentration and got 32.9% in the highest Cd group. Caspase-9 and caspase-3 activities increased in the lower Cd treatment groups but no changes in the higher Cd concentration groups (comparing to the control group). The activity of caspase-8 did not change significantly. No significant change in the content of mitochondrial cytochrome c (cyt c) in Cd exposed groups except the decrease in the 56.98 mg/L group. In crabs treated with 3.56, 7.12 and 14.25 mg/L Cd, hyperpolarization of mitochondrial membrane potential (Δψ m) significantly increased. These results implied that apoptosis in the hepatopancreas induced by Cd occurrs through the mitochondrial caspase-dependent pathway. However, whether there are other apoptotic pathways needs to be studied further.

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Oxidative damages by cadmium and the protective effects of low-molecular-weight chitosan in the freshwater crab (Sinopotamon yangtsekiense Bott 1967)

Cited by 28 publications

References 29 publications

In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill

In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill

Characterization of a Chitosanase from Jelly Fig (Ficus awkeotsang Makino) Latex and Its Application in the Production of Water-Soluble Low Molecular Weight Chitosans

Cd-Induced Apoptosis through the Mitochondrial Pathway in the Hepatopancreas of the Freshwater Crab Sinopotamon henanense

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