Oxidative degradation perturbs physico-chemical properties of hemoglobin in cigarette smokers: a threat to different biomolecules

Biswas, Payel; Seal, Paromita; Sikdar, Jyotirmoy; Haldar, Rajen

doi:10.1080/08958378.2021.1991529

Cited by 2 publications

(4 citation statements)

References 36 publications

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“…Limitation of the study: as described in the Methods section, we used blood samples from young (18-37 years), nonsmoker healthy men with blood type O+ to minimize the heterogeneity of the subject population and potential side factors that can affect RBC deformability [17,[72][73][74][75].…”

Section: Discussionmentioning

confidence: 99%

“…As in a previous study [40], blood samples were collected from young, nonsmoker, healthy male volunteers (18-37 years) with blood type O+ to obtain a homogeneous population. This selection of volunteers was made to minimize the influence of external factors on the interaction of hemoglobin with the membrane and the cell deformability [17,[72][73][74][75].…”

Section: Rbc Sample Sourcesmentioning

confidence: 99%

“…This extremely concentrated protein solution is highly viscous [ 9 ] and exhibits inhomogeneous distribution in the cytosol [ 10 , 11 , 12 ]. In normal RBCs, the main part of Hb is dissolved in the cytosol, while 2–10% is bound to the inner membrane layer [ 13 , 14 , 15 , 16 ] and makes up about 20% of the membrane mass [ 17 ]. The attachment of Hb to the RBC’s membrane’s inner surface [ 18 , 19 , 20 ] and the enhanced hemoglobin–membrane association in sickle RBCs [ 21 , 22 , 23 ] have been detected by analysis of the membrane composition of RBC ghosts.…”

Section: Introductionmentioning

confidence: 99%

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Hemoglobin Binding to the Red Blood Cell (RBC) Membrane Is Associated with Decreased Cell Deformability

Barshtein,

Livshits,

Gural

et al. 2024

IJMS

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The deformability of red blood cells (RBCs), expressing their ability to change their shape as a function of flow-induced shear stress, allows them to optimize oxygen delivery to the tissues and minimize their resistance to flow, especially in microcirculation. During physiological aging and blood storage, or under external stimulations, RBCs undergo metabolic and structural alterations, one of which is hemoglobin (Hb) redistribution between the cytosol and the membrane. Consequently, part of the Hb may attach to the cell membrane, and although this process is reversible, the increase in membrane-bound Hb (MBHb) can affect the cell’s mechanical properties and deformability in particular. In the present study, we examined the correlation between the MBHb levels, determined by mass spectroscopy, and the cell deformability, determined by image analysis. Six hemoglobin subunits were found attached to the RBC membranes. The cell deformability was negatively correlated with the level of four subunits, with a highly significant inter-correlation between them. These data suggest that the decrease in RBC deformability results from Hb redistribution between the cytosol and the cell membrane and the respective Hb interaction with the cell membrane.

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Section: Discussionmentioning

confidence: 99%

Section: Rbc Sample Sourcesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hemoglobin Binding to the Red Blood Cell (RBC) Membrane Is Associated with Decreased Cell Deformability

Barshtein,

Livshits,

Gural

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…This increased content of membrane-bound Hb reduces the elasticity of erythrocyte membrane. 37,38 Moreover, band3 clustering also reduces the life span of erythrocytes. 9 Notably, the Native-PAGE of Hb showed no distinct alterations of its native form of both groups (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and biophysical characterization of hemoglobin of erythrocytes from patients suffering from iron deficiency anemia: A pilot study.

Roy,

Basak,

Biswas

et al. 2024

Ind. J. Physiol. Allied Sci.

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Background: Iron deficiency anemia (IDA) is a substantial global health concern, affecting nearly 1 billion individuals worldwide, and women are the more vulnerable due to their various reproductive phases. This study examines physicochemical aspects of erythrocytes in IDA women, especially in reproductive-age women in the North 24 Parganas district of West Bengal. Methods: Fifteen IDA women and eight healthy women aged 25 to 45 years were selected, and only ten volunteers fulfilled the IDA criteria. Observations: Scanning electron microscopic study revealed the formation of echinocytes, indicating asymmetry in the lipid bilayer of the erythrocyte membrane. Although native-PAGE did not show any remarkable change in hemoglobin structure, absorption spectra indicated structural abnormality in the globin chain of hemoglobin. Increased methemoglobin (metHb) formation rate (higher co-oxidation rate) and increased metHb content in the membrane-bound hemoglobin are clear indications of the alteration of physicochemical property of hemoglobin, which may affect erythrocyte life span in IDA. Conclusion: The hemoglobin of IDA individuals is not only quantitatively low, but the quality is also compromised by its structure, and the morphology of erythrocytes is also altered.

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Oxidative degradation perturbs physico-chemical properties of hemoglobin in cigarette smokers: a threat to different biomolecules

Cited by 2 publications

References 36 publications

Hemoglobin Binding to the Red Blood Cell (RBC) Membrane Is Associated with Decreased Cell Deformability

Hemoglobin Binding to the Red Blood Cell (RBC) Membrane Is Associated with Decreased Cell Deformability

Biochemical and biophysical characterization of hemoglobin of erythrocytes from patients suffering from iron deficiency anemia: A pilot study.

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