Oxidative DNA damage in patients with cataract

Ateş, Orhan; Hakan, Hamit; Kocer, Ibrahim; Baykal, Orhan; Salman, İlknur Akyol

doi:10.1111/j.1755-3768.2009.01583.x

Cited by 37 publications

(38 citation statements)

References 38 publications

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“…14,18 Oxidative stress is proved to cause DNA damage and subsequent cataract formation. 19,20 Blue Mountain Eye Study proved antioxidants intake, including beta-carotene, zinc, and vitamins A, C, and E, reduced nuclear sclerosis cataract formation. 21 Sorte et al 20 examined DNA damage amounts in lens epithelial cells right after cataract surgery and found that DNA damage products were noted maximally in C cataract subject samples.…”

Section: Eyementioning

confidence: 99%

Correlation between microRNA-34a levels and lens opacity severity in age-related cataracts

Chien

Chen

Liu

et al. 2013

Eye

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Purpose MicroRNA 34a (miR-34a) is involved in regulating tissue senescence. However, the role of miR-34a in age-related cataracts is unclear. In this study, we evaluated the correlations among the severity of lens opacity, patient age, and miR-34a expression level in the lens epithelium of age-related cataracts for clarifying the role of miR-34a in the lens senescence. Methods This study was carried as a case control study in the Department of Ophthalmology, Taipei Veterans General Hospital, Taiwan. We recorded age of each patient at the time of their cataract surgery and information regarding lens opacity according to a modified version of the Lens Opacities Classification System III. Correlations among age, lens opacity, and miR-34a expression levels were evaluated. Results This study evaluated 110 patients with a mean age of 73.19 years (SD±10.2). Older patients had higher nuclear cataract (NC), cortical (C), and posterior subcapsular cataract (P) scores (one-way analysis of variance (ANOVA), Po0.05). miR-34a expression levels were significantly different between each age group (ANOVA post hoc Bonferroni's test, Po0.001), and there were moderate correlations between high NC, C, and P cataract scores and high miR-34a levels (Pearson correlation coefficient; R ¼ 0.606, 0.575, and 0.515, respectively). Conclusions The current study demonstrated positive correlations between high miR-34a levels and high lens opacity severity in NC, C, or P cataracts. These results suggest that miR34a expression has a role in lens senescence.

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Section: Eyementioning

confidence: 99%

Correlation between microRNA-34a levels and lens opacity severity in age-related cataracts

Chien

Chen

Liu

et al. 2013

Eye

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“…Previous studies have shown a decrease in the systemic level of antioxidant enzymes such as superoxide dismutase and catalase and an increase in oxidative degradation products in cataract patients [4,5,6]. The biomarker of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), significantly increased in leukocytes and serum of cataract patients [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…The biomarker of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), significantly increased in leukocytes and serum of cataract patients [4,5]. Recently, several studies reported the association between genetic polymorphisms of antioxidant enzymes/DNA repair enzymes [7,8,9,10,11] and ARC.…”

Section: Introductionmentioning

confidence: 99%

DNA Damage in Lens Epithelial Cells and Peripheral Lymphocytes from Age-Related Cataract Patients

Zhang

Wu²,

Yang³

et al. 2014

Ophthalmic Res

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Background: Oxidative DNA damage may be one of the etiologies of age-related cataract (ARC). We quantified DNA damage in lens epithelial cells (LECs) and peripheral blood lymphocytes of ARC. Methods: A total of 64 patients with different types of ARC and 23 control subjects were enrolled. Fresh LECs and peripheral lymphocytes were collected and DNA damage was evaluated by alkaline comet assay. The percentage of DNA in the tail of comets (%Tail DNA) and the olive tail moment (OTM) were calculated by CASP software. Results: The results showed the %Tail DNA and OTM in LECs and lymphocytes in the overall cataract patient group were significantly higher than those in the control subjects. The %Tail DNA and OTM of LECs and lymphocytes showed no differences among cortical, nuclear and posterior subcapsular cataracts. The %Tail DNA and OTM in LECs were significantly lower than those in lymphocytes but a significant correlation of the DNA damage was found between them. Conclusion: We concluded that DNA damage in lens and peripheral blood lymphocytes increased in ARC. The results imply that local and systemic oxidative DNA damage might play certain roles in ARC pathogenesis.

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“…This result was in line with a study that found elevated levels of 8-OH-Gua, a marker of oxidative DNA damage, in the leukocytes of patients with cataract. 40 However, we did not find the correction of the DNA damage with the genotypes. This may be due to the nature of the local lesion (lens) in ARC.…”

Section: Discussionmentioning

confidence: 53%

The Associations between Single Nucleotide Polymorphisms of DNA Repair Genes, DNA Damage, and Age-Related Cataract: Jiangsu Eye Study

Yao

Zhu

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Age-related cataract (ARC) is one of the most common causes of severe visual impairment among the elderly worldwide with four subtypes, such as cortical, nuclear, subcapsular, and mixed types. DNA damage and malfunction of DNA repair are believed to contribute to the pathogenesis of ARC. This study examined the associations of 18 single nucleotide polymorphisms (SNPs) in four DNA repair genes (BLM, WRN, ERCC6, and OGG1) with ARC in Han Chinese from the Jiangsu Eye Study, a population-based epidemiologic study. We also determined the possible functional consequence of the SNPs to DNA damage. METHODS.Eighteen SNPs in four DNA repair genes were genotyped in 789 ARC patients and 531 normal controls from the Jiangsu Eye Study. The Comet assay was to assess the extent of DNA damage in peripheral lymphocytes of selected subjects. RESULTS.The results show that WRN-rs11574311 was initially associated with ARC in general, cortical, and mixed cataracts (P ¼ 0.003, odds ratio [OR] ¼ 1.49; P ¼ 0.001, OR ¼ 1.68; and P < 0.0001, OR ¼ 2.08), BLM-rs1063147 with nuclear cataract (P ¼ 0.03, OR ¼ 1.31), WRN-rs2725383 with cortical cataract (P ¼ 0.01, OR ¼ 1.49), and WRN-rs4733220 and WRN-rs2725338 with mixed cataract (P ¼ 0.04, OR ¼ 0.74; P ¼ 0.003, OR ¼ 0.60). However, the significances of some of the above-cited associations disappeared after multiple testing corrections. WRN-rs11574311 remains associated with cortical and mixed cataract and WRN-rs2725338 with mixed cataract after multiple testing correction. We did not find any correlation between DNA damage of peripheral lymphocytes and SNP types.CONCLUSIONS. We concluded that WRN genes might be involved in ARC pathogenesis in the Han Chinese population. The associations were ARC subtype specific. These findings stress the importance of detailed phenotyping in ARC subtypes, which may be associated with different risk factors and disease mechanisms. (Invest Ophthalmol Vis Sci. 2013;54:1201-1207) DOI:10.1167/iovs.12-10940 A ge-related cataract (ARC) is one of the most common causes of severe visual impairment among the elderly worldwide, rendering the disease a major public health issue.1,2 ARC is a progressive opacification of the ocular lens, leading to visual impairment and blindness. According to the degenerative region of lens, ARC can be classified as nuclear (N), cortical (C), posterior subcapsular (PSC), and mixed type (M). 3 ARC has a multifactorial etiology. Age, sex, smoking, exposure to sunlight, chronic ocular inflammation, estrogen sufficiency or deficiency, and cardiovascular factors may influence ARC predisposition. The strongest evidence came from twin studies demonstrating a heritability of 48% for nuclear cataract 4 and 59% for cortical cataract. 5 The importance of genetic risk factors for ARC was highlighted in several recent studies, which have reported that OGG1, EPHA2, and glutathione S-transferase (GST) polymorphisms are associated with the C type of ARC in the Chinese population. [6][7][8][9] Although the pathophysiology of ARC is far from clear...

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Oxidative DNA damage in patients with cataract

Cited by 37 publications

References 38 publications

Correlation between microRNA-34a levels and lens opacity severity in age-related cataracts

Correlation between microRNA-34a levels and lens opacity severity in age-related cataracts

DNA Damage in Lens Epithelial Cells and Peripheral Lymphocytes from Age-Related Cataract Patients

The Associations between Single Nucleotide Polymorphisms of DNA Repair Genes, DNA Damage, and Age-Related Cataract: Jiangsu Eye Study

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