Oxidative effects of glyphosate on the lipophobic intracellular environment in the microalgae

Ostera, Juan Manuel; Puntarulo, Susana; Malanga, Gabriela

doi:10.32604/biocell.2022.017294

Cited by 3 publications

(5 citation statements)

References 39 publications

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“…The comparison among the effects of the tested oxidative radical scavengers showed that the addition of CAT, GSH, and DF produced the highest percentage of inhibition of the DCFH-DA oxidation rate in the diatoms exposed to DA. GSH is a non-specific antioxidant at the hydrophilic cellular level, and CAT and DF are involved in the metabolism of H 2 O 2 and iron (Fe), respectively [ 4 ]. Thus, an increase in H 2 O 2 generation and alterations in the Fe oxidative metabolism could be suggested as the main effects of the exposure of the microalgae to the biotoxin [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, cells exposed to DA in the LAG phase of growth also exhibited a higher decrease in AH − content, as compared to cells in the EXP growth phase. The different behavior observed among the growth phases could be influenced by the diverse nutrient availability and metabolism in each phase, being more active in the EXP phase [ 51 , 52 ]. To assess the magnitude of the ability of the antioxidant defense network to control the oxidant effects of DA exposure in the cells in the three phases of development, damage markers should be evaluated in the future.…”

Section: Discussionmentioning

confidence: 99%

“…The increase in the natural environment of the content of nitrogen and phosphorus stimulates both the excessive growth of many types of algae and the prevalence of HABs [ 3 ]. These nutrients enter rivers and estuaries from many sources, such as improperly treated wastewater, fertilized lawns, and agricultural runoff, either from fertilized fields, animal wastes, or intense precipitation events [ 4 ]. It was described that long-term changes in water temperatures may allow some species of toxic algae to increase the seasonality of their blooms, or to expand to temperate zones [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum

Cabrera

Puntarulo

González

2023

Life

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Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum

Cabrera

Puntarulo

González

2023

Life

Self Cite

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“…The rapid chlorophyll fluorescence induction curve is a powerful tool for studying and detecting the effects of environmental stress and pollutants on photosynthesis because it can provide extremely abundant information on photosynthetic electron transport [19,25,36]. As single-cell organisms, algae, especially in their photosynthetic system, are very sensitive to stress and pollutants [37,38]. When subjected to stress and pollutants, photosynthetic chlorophyll fluorescence and electron transport parameters change quickly.…”

Section: Discussionmentioning

confidence: 99%

Photosynthesis Responses of Tibetan Freshwater Algae Chlorella vulgaris to Herbicide Glyphosate

Zhang

Chen

et al. 2022

IJERPH

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With the development of agriculture and the widespread application of agrichemicals in Tibet, herbicide residues have become a threat to the ecological safety of Tibetan water bodies. Algae, as the producers in the food chain in water bodies, play an important role in aquatic ecosystems. Therefore, the impact of herbicides on Tibetan algae is of great significance for evaluating ecological health and the protection of Tibetan water ecosystems. In this study, we investigated the inhibitory effect of glyphosate, a herbicide, on the photosynthetic system of Chlorella vulgaris, Tibetan algae, by determining chlorophyll fluorescence and the activity of an antioxidant system. The results revealed that glyphosate at low concentration did not affect the photosynthetic activity of C. vulgaris; however, glyphosate at a high concentration significantly inhibited photosynthetic activity and reduced pigment content. Moreover, high levels of glyphosate also decreased photochemical efficiency and electron transport rate and resulted in ROS accumulation, high SOD activity, and lipid peroxidation. These results suggested that glyphosate could decrease the primary production of aquatic ecosystems and influence their performance. Therefore, reducing the herbicide levels could protect the Tibetan aquatic environment and maintain the health of ecosystems.

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“…The humic acid–metal complexes are well-known for their role in environmental detoxification [ 97 ]. Detoxification of various aquatic pollutants reduces their toxicity on microalgae.…”

Section: Protective Effects Of Humic Substances On Microalgaementioning

confidence: 99%

Humic Substances as Microalgal Biostimulants—Implications for Microalgal Biotechnology

Popa

Lupu

Constantinescu-Aruxandei

et al. 2022

Marine Drugs

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Humic substances (HS) act as biostimulants for terrestrial photosynthetic organisms. Their effects on plants are related to specific HS features: pH and redox buffering activities, (pseudo)emulsifying and surfactant characteristics, capacity to bind metallic ions and to encapsulate labile hydrophobic molecules, ability to adsorb to the wall structures of cells. The specific properties of HS result from the complexity of their supramolecular structure. This structure is more dynamic in aqueous solutions/suspensions than in soil, which enhances the specific characteristics of HS. Therefore, HS effects on microalgae are more pronounced than on terrestrial plants. The reported HS effects on microalgae include increased ionic nutrient availability, improved protection against abiotic stress, including against various chemical pollutants and ionic species of potentially toxic elements, higher accumulation of value-added ingredients, and enhanced bio-flocculation. These HS effects are similar to those on terrestrial plants and could be considered microalgal biostimulant effects. Such biostimulant effects are underutilized in current microalgal biotechnology. This review presents knowledge related to interactions between microalgae and humic substances and analyzes the potential of HS to enhance the productivity and profitability of microalgal biotechnology.

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Oxidative effects of glyphosate on the lipophobic intracellular environment in the microalgae

Cited by 3 publications

References 39 publications

Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum

Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum

Photosynthesis Responses of Tibetan Freshwater Algae Chlorella vulgaris to Herbicide Glyphosate

Humic Substances as Microalgal Biostimulants—Implications for Microalgal Biotechnology

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